Felicitări domnule Prof.din păcate așa procedăm cu toți oamenii deosebiți din tara asta.Va rog sa nu renunta- ți la munca d- stra.Va doresc multă sănătate și să se trezească multi romani,sa vă sprijine. Sunt de acord să găsiți o modalitate pt ca oamenii care vor să sprijine activitatea d- stra,sa o poată face.
Ar fi trebuit sa se deplaseze la Timișoara încă din martie tot guvernul sa afle cum poate fi ajutat institutul sa realizeze vaccinul, care apoi sa fie produs la Cantacuzino și sa devină o mina de aur pentru România.
Prețuire și respect! Mulțumesc cu recunoștință.Am mai multa încredere în vaccinul românesc,îmi amintesc de cele făcute de Cantacuzino când eram copil.Doamne ajuta! 🙏
TOATĂ STIMA STIMATE DOMN..! NU VA AJUTA MASONII FAPTUL CA MOR FARMACIILE..! DOAMNE AJUTANE ȘI VA RUGAM SA LIPTATI PENTRU ROMANII DE BUNA CREDINȚĂ! DOAMNE AJUTA!
AVEM SPECIALIȘTI DE TOP CĂUTAȚI DE TOATE FIRMELE DE TOP DIN LUME , MEDICI DE MARCĂ CARE ÎI PIERDEM DEOARECE ACEȘTIA PLEACĂ ACOLO UNDE SUNT VALORIZAȚI ...
Trebuia ca, conducătorii noștrii să cumpere vaccinuri din afară, ca să i îmbogățească pe cei bogați. Bogați care bine ințeles, ne numesc in functii marionetele care ne "conduc".
Pe site-ul The National Pulse - BGI Genomics-the Chinese Communist Party-linked genomics firm flagged by U.S. officials as “mining” the DNA of Americans-has collaborated extensively with the Bill & Melinda Gates Foundation, The National Pulse can reveal. “We were taken aback. We never thought we would find such an out-of-the-box approach. They are in their own league - open and liberal. Most people only see them as a service provider for DNA analysis. It is the database they are building that will make them formidable.” Pe site-ul Scitable by Nature Education - “We were taken aback. We never thought we would find such an out-of-the-box approach. They are in their own league - open and liberal. Most people only see them as a service provider for DNA analysis. It is the database they are building that will make them formidable.” Making DNA from RNA: Reversal of the Central Dogma ˝The central dogma, enunciated by Crick in 1958 and the keystone of molecular biology ever since, is likely to prove a considerable over-simplification. That is the heretical but inescapable conclusion stemming from experiments done in the past few months in two laboratories in the United States." --"Central Dogma Reversed," Nature, June 27, 1970 The so-called central dogma of molecular biology states that all genetic information flows in one direction: from DNA to RNA through the process of transcription, and then from RNA to protein through the process of translation (Crick, 1958). For over a decade, the central dogma was thought to be a universal truth--in other words, researchers believed that genetic information always flowed in this order, otherwise it could not be passed along. In 1970, however, the two experiments mentioned in the Nature quote--one conducted by David Baltimore, then of the California Institute of Technology in Pasadena, and the other by Howard Temin and Satoshi Mizutani, then of the University of Wisconsin in Madison--called this belief into question. Specifically, these researchers independently published scientific papers demonstrating that RNA tumor viruses contain enzymes that use viral RNA as a template for the synthesis of DNA, thereby reversing the direction of transcription (Baltimore, 1970; Temin & Mizutani, 1970). Not only did these two experiments challenge the validity of the central dogma, but they also laid the foundation for a series of technological developments that eventually earned reverse transcription and the synthesis of complementary DNA, or cDNA, central places in the molecular biologist's toolbox. Discovering Reverse Transcription During the late 1960s, Baltimore, Temin, and Mizutani were each driven by unanswered questions about how RNA viruses transformed healthy cells into tumor cells. They knew that transformation ensued when healthy cells incorporated DNA from the external environment (in this case, RNA tumor virus DNA) into their genomes. But how could a eukaryotic cell incorporate DNA from a virus that didn't have any DNA? The experiments supporting the existence of this DNA polymerase produced data that revealed the following: The DNA polymerase only incorporated deoxyribonucleotides, not ribonucleotides, into its product. The product itself "behaved" like DNA--in other words, it was sensitive to treatment by deoxyribonucleases but not ribonucleases. The RNA itself was the template, as shown by the fact that treatment of virions with ribonucleases destroyed the ability of the polymerase to incorporate radioactively labeled nucleotides. How PCR Works Basically, PCR is DNA replication on a grander scale. The polymerase chain reaction relies on the use of several essential chemical ingredients, including the following: A DNA polymerase A small amount of DNA to serve as the initial template The four deoxyribonucleotides to serve as the substrates for the DNA polymerase and the raw ingredients of the new DNA molecules A few necessary ions and salts A pair of primers with exposed 3′-OH groups that will bind to the particular sequence of interest in the DNA template Within just a few minutes, the chain reaction doubles the number of DNA molecules for the specific sequence of interest. The reaction--and the doubling--are repeated every few minutes, as long as the PCR is allowed to proceed. This leads to the creation of a large amount of DNA in a relatively short period. For example, even if the process were started with just a single DNA molecule, it would result in more than 1 billion molecules at the end of just 30 PCR reactions or cycles. Summary Thus, both cloning of expressed genes and PCR continue to serve as essential tools for genetic researchers. Cloning--which involves the creation of DNA from mRNA and thus represents a reversal of the central dogma--is particularly useful when scientists aren't able to isolate the DNA template of a sequence of interest. In addition, because this method relies on mRNA rather than DNA, it provides an excellent means for studying the differences in gene expression in different cells at different points in development. PCR, on the other hand, is more akin to "traditional" DNA synthesis in that it requires the presence of an initial DNA (rather than RNA) template. The primary advantage of PCR is its speed--even if researchers begin with only a single segment of DNA, they can produce literally billions of molecules within a matter of hours. As with all technologies, scientists continue to improve both PCR and the cloning process, thereby ensuring that these methods will play a role in genetic breakthroughs for years to come.
Felicitări domnule Prof.din păcate așa procedăm cu toți oamenii deosebiți din tara asta.Va rog sa nu renunta- ți la munca d- stra.Va doresc multă sănătate și să se trezească multi romani,sa vă sprijine. Sunt de acord să găsiți o modalitate pt ca oamenii care vor să sprijine activitatea d- stra,sa o poată face.
Multumiri pentru toate explicatiile date de domnul doctor, marelui public.
Minunat dnule dr și mulțumim d-nei care v a invitat... Multa răbdare și sanatate!
Am ascultat .Dzeu.sa apere poporul sau.Succes pentru dl.doctor Paunescu cu sanatate.
Binecuvinteaza l Doamne pe acest om minunat .
Ar fi trebuit sa se deplaseze la Timișoara încă din martie tot guvernul sa afle cum poate fi ajutat institutul sa realizeze vaccinul, care apoi sa fie produs la Cantacuzino și sa devină o mina de aur pentru România.
Mulțumesc ptr toate explicațiile Doamne ajuta!!
Prețuire și respect! Mulțumesc cu recunoștință.Am mai multa încredere în vaccinul românesc,îmi amintesc de cele făcute de Cantacuzino când eram copil.Doamne ajuta! 🙏
Binecuvinteaza-l Doamne pe acest minunat om!
bravo Domnule Doctor Virgil,,
Felicitări domnule doctor, oameni ca dumneavoastră nu sunt ajutați să facă ceva pentru oamenii din Romania.
TOATĂ STIMA STIMATE DOMN..! NU VA AJUTA MASONII FAPTUL CA MOR FARMACIILE..! DOAMNE AJUTANE ȘI VA RUGAM SA LIPTATI PENTRU ROMANII DE BUNA CREDINȚĂ! DOAMNE AJUTA!
Acest doctor va revoluționa medicina. O să mai auzim de el.
Doamne Ajuta
Un geniu acest medic!
Să.l ajute Dumnezeu🙏
Felicitări, din inimă!
Am încredere în vaccinul dvs.Aveți nevoie de voluntari? Mă ofer.
AVEM SPECIALIȘTI DE TOP CĂUTAȚI DE TOATE FIRMELE DE TOP DIN LUME , MEDICI DE MARCĂ CARE ÎI PIERDEM DEOARECE ACEȘTIA PLEACĂ ACOLO UNDE SUNT VALORIZAȚI ...
Tot ce-i românesc nu piere!
Dați un număr de telefon la care vă putem contacta, sunt interesat.
daca l-ati testat pe dvs...de ce nu este aprobat la ora actuala 13 01 2021...domnule profesor?! trebuia sa asteptam la PFIZER
Trebuia ca, conducătorii noștrii să cumpere vaccinuri din afară, ca să i îmbogățească pe cei bogați. Bogați care bine ințeles, ne numesc in functii marionetele care ne "conduc".
Nu li se dau aprobările. Intenționat se face asta.
"Probabil că virusul a apărut de la lilieci!" Probabil de la lilieci cu două picioare! Probabil!
Păunescu președinte! Nu contează care.
Asta e ,Romania nu va dat bani .... ticalosii
Faceți tratament nu vaccin ! Pentru toate bolile exista vaccin ? Despre tratament de ce nu vorbește nimeni ?
Bravo!
Pe site-ul The National Pulse - BGI Genomics-the Chinese Communist Party-linked genomics firm flagged by U.S. officials as “mining” the DNA of Americans-has collaborated extensively with the Bill & Melinda Gates Foundation, The National Pulse can reveal.
“We were taken aback. We never thought we would find such an out-of-the-box approach. They are in their own league - open and liberal. Most people only see them as a service provider for DNA analysis. It is the database they are building that will make them formidable.”
Pe site-ul Scitable by Nature Education - “We were taken aback. We never thought we would find such an out-of-the-box approach. They are in their own league - open and liberal. Most people only see them as a service provider for DNA analysis. It is the database they are building that will make them formidable.”
Making DNA from RNA: Reversal of the Central Dogma
˝The central dogma, enunciated by Crick in 1958 and the keystone of molecular biology ever since, is likely to prove a considerable over-simplification. That is the heretical but inescapable conclusion stemming from experiments done in the past few months in two laboratories in the United States."
--"Central Dogma Reversed," Nature, June 27, 1970
The so-called central dogma of molecular biology states that all genetic information flows in one direction: from DNA to RNA through the process of transcription, and then from RNA to protein through the process of translation (Crick, 1958). For over a decade, the central dogma was thought to be a universal truth--in other words, researchers believed that genetic information always flowed in this order, otherwise it could not be passed along. In 1970, however, the two experiments mentioned in the Nature quote--one conducted by David Baltimore, then of the California Institute of Technology in Pasadena, and the other by Howard Temin and Satoshi Mizutani, then of the University of Wisconsin in Madison--called this belief into question. Specifically, these researchers independently published scientific papers demonstrating that RNA tumor viruses contain enzymes that use viral RNA as a template for the synthesis of DNA, thereby reversing the direction of transcription (Baltimore, 1970; Temin & Mizutani, 1970). Not only did these two experiments challenge the validity of the central dogma, but they also laid the foundation for a series of technological developments that eventually earned reverse transcription and the synthesis of complementary DNA, or cDNA, central places in the molecular biologist's toolbox.
Discovering Reverse Transcription
During the late 1960s, Baltimore, Temin, and Mizutani were each driven by unanswered questions about how RNA viruses transformed healthy cells into tumor cells. They knew that transformation ensued when healthy cells incorporated DNA from the external environment (in this case, RNA tumor virus DNA) into their genomes. But how could a eukaryotic cell incorporate DNA from a virus that didn't have any DNA?
The experiments supporting the existence of this DNA polymerase produced data that revealed the following:
The DNA polymerase only incorporated deoxyribonucleotides, not ribonucleotides, into its product.
The product itself "behaved" like DNA--in other words, it was sensitive to treatment by deoxyribonucleases but not ribonucleases.
The RNA itself was the template, as shown by the fact that treatment of virions with ribonucleases destroyed the ability of the polymerase to incorporate radioactively labeled nucleotides.
How PCR Works
Basically, PCR is DNA replication on a grander scale. The polymerase chain reaction relies on the use of several essential chemical ingredients, including the following:
A DNA polymerase
A small amount of DNA to serve as the initial template
The four deoxyribonucleotides to serve as the substrates for the DNA polymerase and the raw ingredients of the new DNA molecules
A few necessary ions and salts
A pair of primers with exposed 3′-OH groups that will bind to the particular sequence of interest in the DNA template
Within just a few minutes, the chain reaction doubles the number of DNA molecules for the specific sequence of interest. The reaction--and the doubling--are repeated every few minutes, as long as the PCR is allowed to proceed. This leads to the creation of a large amount of DNA in a relatively short period. For example, even if the process were started with just a single DNA molecule, it would result in more than 1 billion molecules at the end of just 30 PCR reactions or cycles.
Summary
Thus, both cloning of expressed genes and PCR continue to serve as essential tools for genetic researchers. Cloning--which involves the creation of DNA from mRNA and thus represents a reversal of the central dogma--is particularly useful when scientists aren't able to isolate the DNA template of a sequence of interest. In addition, because this method relies on mRNA rather than DNA, it provides an excellent means for studying the differences in gene expression in different cells at different points in development. PCR, on the other hand, is more akin to "traditional" DNA synthesis in that it requires the presence of an initial DNA (rather than RNA) template. The primary advantage of PCR is its speed--even if researchers begin with only a single segment of DNA, they can produce literally billions of molecules within a matter of hours. As with all technologies, scientists continue to improve both PCR and the cloning process, thereby ensuring that these methods will play a role in genetic breakthroughs for years to come.
Eu imi fac vaccin numai Românesc
De unde stii ca dracul nu îşi vâră coada si acolo?
@@carmenmoldoveanu4897 🤣🤣🤣
nu se aude bine
Pune căştile.
@@carmenmoldoveanu4897 mersi,asta am facut pana la urma