Are you able to paste a section of the bed file here? I have never seen the situation you describe. What reference genome are you using for your experiment?
@@bioinformatics1015 chr10 0 73600 chr10 73600 73800 chr10 73800 74400 chr10 74400 76000 So in the first line,. that's really from chr10:0-736 bp, line 2 is chr10:736-738bp, and so on. Why does it end in '00'? Thanks!
@@quililank Since I am not sure how the bed was created, I would assume the extra '00's are a mistake. If you are certain it is supposed to be chr10:0-736, I would recommend stripping off the extra 0s in the file before use. Let me know if you have any more questions!
![[WEBINAR] Intro to Bioinformatics Pipelines for ChIP-Seq](/img/n.gif)
is it possible to convert .tab output from HTSeq count to .bed file for visualization of coverage on igv?
I don't understand where the information about the sequence is tho?! Like where is the sequence?
Please do more videos
Hi! Why does my start and end position end in'00'? Is this necessary?
Are you able to paste a section of the bed file here? I have never seen the situation you describe. What reference genome are you using for your experiment?
@@bioinformatics1015
chr10 0 73600
chr10 73600 73800
chr10 73800 74400
chr10 74400 76000
So in the first line,. that's really from chr10:0-736 bp, line 2 is chr10:736-738bp, and so on.
Why does it end in '00'? Thanks!
@@bioinformatics1015 I'm using hg38
@@quililank Since I am not sure how the bed was created, I would assume the extra '00's are a mistake. If you are certain it is supposed to be chr10:0-736, I would recommend stripping off the extra 0s in the file before use. Let me know if you have any more questions!
Got a question, the exons you showed in the video as a box. Are those exons represent on ssDNA strand or dsDNA?
ds
Superb! thank you
hope you continue the channel man; useful content!
Thanks for the clear explanation!