HPLC in Pharmaceutical industry High performance liquid chromatography Interview Question & answers

HPLC in Pharmaceutical industry l High performance liquid chromatography l Interview Question and answers
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Questions covered:
Q. What does HPLC stand for or what is full form of HPLC ?
Q. What is the primary purpose of HPLC in the pharmaceutical industry ?
Q. What is the mobile phase in HPLC ?
Q. Define stationary phase in HPLC ?
Q. What is the typical particle size of HPLC column packing material ?
Q. Explain the purpose of the detector in HPLC ?
Q. What is the retention time in HPLC ?
Q. Why is gradient elution used in HPLC ?
Q. What is the purpose of the guard column in HPLC ?
Q. Explain the concept of peak asymmetry in chromatography ?
Q. How does temperature affect HPLC separation?
Q. What is the significance of the injection volume in HPLC ?
Q. What is the role of the pre-column in HPLC?
Q. Why is isocratic elution used in HPLC ?
Q. What is the purpose of the equilibration step in HPLC ?
Q. Explain the term "dead volume" in HPLC ?
Q. How does the flow rate affect HPLC separation ?
Q. What is the role of a guard column in HPLC ?
Q. Define the term "selectivity" in HPLC ?
Q. What is the purpose of the sample solvent in sample preparation for HPLC ?
Q. Why is HPLC preferred over other chromatographic techniques in pharmaceutical analysis ?
Q. How does pH affect HPLC separations ?
Q. Explain the concept of peak capacity in HPLC ?
Q. What is the significance of the detector wavelength in HPLC ?
Q. How does column length impact HPLC separation ?
Q. What is the purpose of a sample loop in the injection system of HPLC ?
Q. Explain the concept of "system suitability" in HPLC ?
Q. What is the significance of the cut-off wavelength in UV detection for HPLC ?
Q. How does column diameter affect HPLC performance ?
Q. Why is it essential to degas the mobile phase in HPLC ?
Q. What are the limitations of HPLC technique used in the pharmaceutical industry?
Q. Which USP chapter talk about HPLC in the pharmaceutical industry and what is title of that chapter ?
Q. Which guidelines are commonly referred for HPLC in the pharmaceutical industry ?
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