Plasmid DNA Extraction (CsCI Isolation)

( www.abnova.com ) - CsCI isolation is a widely used method for isolating highly pure circular plasmid DNA in relatively high yield from bacterial cells. This video demonstrates the procedures for CsCI isolation method. More videos at Abnova www.abnova.com

[@Paganforge]

When removing the EtBr with n-butanol, is there an intermediate step not shown, such as a brief centrifiguation? How do you get the n-butanol out of the mix without removing the DNA?

[@tendoeschate]

it should be 150 microlitre EtBr

[@Sims_beat]

Really helpful 👍🏻👍🏻👍🏻