TIRFM - Total Internal Reflection Fluorescence Microscopy by LWarning

Informational video about TIRFM. Please note that, although not specified in this video, the penetration depth of the evanescent wave is dependent on the TIRF angle used and excitation wavelength. Generally, it is between 30-300 nm (video states ~100 nm).

[@eunseongjun9298]

Thank you for making this video! I learned a lot by spending a few minutes.

[@linus9456]

this is literally the only video that explains this topic clearly, thank you so much!

[@Toothfairy1018]

literally best video on this subject. the rest either suck or are long and tedious to understand. great job and thank you!

[@kseniiazavatskaia2905]

Thanks a lot for the video! Simple and concise - just what I needed.

[@sagarmohanpawar4057]

Great ,small and informative video would love to see more

[@Medic81]

Wow, exactly what I needed. Very clear and concise.

[@traxin2107]

Nice work

[@gemechumekonnen8786]

Ver clearly described. Thank you!

[@SF-fb6lv]

Very nice explanation, thanks!

[@sunnycai7362]

very clear and helpful, thanks a lot!

[@quililank]

GREAT VIDEO!!! I understood it easily and quickly :D

[@sahilsharma9385]

Such a great video!!!

[@lotharmayring6063]

nothing else than oblique fluorescence illumination

[@nazmidzulkifli4946]

thank you so much!!!!

[@Abbyblackblack]

So clear thank you!

[@MV-nq7rh]

thank you

[@alexcopperman2065]

Amazing video!

[@angelamoreno9621]

SO USEFUL, thank you!!!!! :)

[@yizimao4802]

This is really helpful! Thank you!

[@naemibickmeier4013]

So helpful. Thank you

[@pratitidas]

Hey laura why dont you make more videos??

[@prakashjoshi6796]

What is the resolution of the microscopy in xy direction

[@azharulislam882]

thanks a lot.

[@ketkipawar3898]

well explained. Thank you! :)

[@xuli423]

clear!

[@DumbEnough2Try]

Ur the best