Great stuff. Hand-held cutting with a new razor blade under a 20x (2x objective + 10x ocular ) stereomicroscope can also give exceptionally good results with some training. This was always the best solution for me, I preferred over a hand-held microtome.
New idea using clear glue. I have an extensive collection of pollen (my special study). I use gelatin sealed with varnish rings around the covers lips. Would Elmer’s work instead.
Thank you so much, another interesting and educational live stream, I don’t have a 3d printer but would like to use razor blades with my microtome, do you know of anywhere I can buy something like the plastic blade holder you made?
I watched someone cutting stem segments freehand using a razor, and it seemed much less of a hassle than the microtome. What is you opinion on this, and do you have a preference?
Have you ever tried food colouring in place of Methylene blue?
Do you think that it works?
Great stuff. Hand-held cutting with a new razor blade under a 20x (2x objective + 10x ocular ) stereomicroscope can also give exceptionally good results with some training. This was always the best solution for me, I preferred over a hand-held microtome.
this are no cell-membranes by plasmolysis. This are simply the cell-walls which you cutted
I thougt the same
outstanding detailed instructional video. Thank you! great tip with the Elmer's glue mounting medium
I like it so much
New idea using clear glue. I have an extensive collection of pollen (my special study). I use gelatin sealed with varnish rings around the covers lips. Would Elmer’s work instead.
Thank you so much, another interesting and educational live stream, I don’t have a 3d printer but would like to use razor blades with my microtome, do you know of anywhere I can buy something like the plastic blade holder you made?
Hi Oliver! Have you ever considered disassembling maybe a spare or broken objective and putting it under the microscope?
Yes, but I did not put it under the microscope. I took one apart. Check this out: th-cam.com/video/OiRxYpvfMIw/w-d-xo.html
I watched someone cutting stem segments freehand using a razor, and it seemed much less of a hassle than the microtome. What is you opinion on this, and do you have a preference?
Amazing .....
Morning
totaly overstained, no clearing