how to do circular dichroism || practical biochemistry
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- เผยแพร่เมื่อ 10 ก.พ. 2025
- cd is a popular technique for determining protein secondary structure. if you have any questions leave a comment.
optimal protein concentration for cd is about 0.2-0.3 mg/ml. If you go higher you will get a noisy tail and if you go lower the signal will not be great.
if you like it subscribe and check this video:
• 🧪Thermal Denaturation ...
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It was really well explained and a nice video. It had helped me a lot. Thank you very much.
Thank you for watching. I am glad it was helpful.
Cool. Thnx
it was really nice explaination.
Thank you
It was so helpful...🤩...please continue making videos like this..😊
Thank you so much!
Thank you,This was very helpful.
Love from India.💛
Very helpful! Thank you!
simple and humble
thank you @Biochemist Melo
You are welcome 😊 thanks for watching
Awesome video 👍
Merci
Thank you 💖 it really explains a lot
You are very welcome
Thank you for this.
You are very welcome
THANK YOU!
🙏🙏
Thank you Melo
Very welcome
thanks
Which lab techniques would you like to see next?
Nice Video!
Can you show us how to make a IR spectroscopy of a protein?
@@giacomovenditti8034 yes I will definitely make a video next time I do it
Can’t wait to see it! Thanks!!
tryptophan fluorescence
Can you give some basic technics to handle in microbiology laboratory
I need more videos
Thank you so much. Working on a peptide synthesis video.
Hi, thank you for this excelent video! I have some questions (probably silly ones). Situation: a experiment where i used a buffer as blank and the software was set to execute baseline correction. Does it means the further spectra obtained from samples will be already subtracted from buffer? Do i still need to click on baseline to manually adjust it after smoothing? If so, how i do the adjusting?
Thank you so for much posting this video! Can you please tell me what concentration of protein was used?
I used 0.3 mg/mL
Would you mind prepare videos of full process? And things about NMR and x ray crystallography would be usefull. I mean the full protocols in lab
Yes. I am planning to make videos on NMR but it would be broken down in multiple videos. There are too many experiment types, and solution vs solid state nmr.
I recently did CD and the jasco analysis say it has no beta sheets but when I use k2d2 software it shows beta sheets. any idea why it is showing such variations. thank you. great video btw❤❤❤
Thank you. Yes the softwares are not 100% accurate and my supervisor doesn't actually trust it for accurately determining which exact percent is which secondary structure. It comes down to what you know about your protein and getting additional data from other techniques to see which one makes more sense.
Hi Biochemist Melo. Can I please have the parameters that you used
Were you using a spacer so that the cuvette fits properly and if so does the Cuvette go to the right or left of the spacer? Also, any more videos on CD would be greatly appreciated. It's sort of embarrassing but we have had a Jasco-1500 for about two years and haven't had time to learn how to use it so this video was fantastic.
I'm not sure I will check on it and let you know. I will do a video on thermal denaturation using cd soon. You should definitely take advantage of your instrument 😊.
How do you read the flow gauge? It Should be about 3 liters per minute correct?
What would it look like if the protein has a rossmann fold?
It is possible to estimate the percentages of alpha and beta mix in a protein using cd but CD is not the most accurate way especially if it's the only technique you are using. Chemical shifts from nmr will tell you abt secondary structure more accurately if your protein is not huge. It's always best to do both if you can.
www.pnas.org/content/112/24/E3095
you fine af
Thank you