Nanopore MinION starter-pack video

This video shows how to prepare a rapid library (based on starter pack) and run it on MinION apparatus
prepared in Dr. Matan Oren Lab at Ariel University by Svetlana Maslov and David Bern

[@asvenesharma8777]

Very well demonstrated.. keep it up..

[@bockwurstesser1046]

8:11 "gently open the Spot ON cover, to expose the loading airport" lol.
Keep up the good work👍🏾

[@bijigd8957]

Thanks. very useful

[@davidbogler4565]

Nice video. Is this done using only the ligation kit reagents in the startup package? What kind of DNA are you sequencing (PCR product)?

[@easywang2257]

Thank you for your detailed explanation
According to your experience, if you perform a whole transcriptome experiment on mouse cells and add 12 barcodes, can a flow cell measure the differential expression data of the whole transcriptome?
Thank you so much!

[@anweshag91]

Hi. Thank you for the detailed video. If I want to get 0.5GB data per sample, how many samples can I combine and load in one flow cell?

[@magiclee9482]

Thank you for the video tutorial! Do you have everything written down?

[@matanoren2220]

All protocols are available in ONT community

[@wardaamjad8311]

@@matanoren2220 dear sir, what is ont community? From where we can find?

[@ichrafhammami797]

​@@wardaamjad8311nanopore Oxford technology

[@fatimakhalid7446]

Very nice vdo.

[@GraceBeyondRace]

Pls upload new video regarding this tech if possible.

[@MrHassan213]

its possible send the steps protocol?

[@shivanikhataokar210]

What amount of FLT did you guys put in the sample?

[@dr.whocares8702]

One question, is the pcr really necessary? I was told it could be done without it.

[@mookfaru835]

They are probably maximizing thier return

[@SofiasBio]

What kind of flow cell is this?

[@arctic618]

i wonder myself if this is anonymous , do you need to be connected on internet to run it ? I v heard that informations comunicate on theirs servers.

[@matanoren2220]

It is anonymous and it can be run without internet (other then updates )

[@Selahmescudi]

is this machine able to sequence a whole human genome?

[@sonyxperiasmk]

How many Times can you use a flow cell?

[@thoughtworn]

Just once. But you can multiplex samples through barcoding

[@matanoren2220]

Potentially several times until performance is going down. You can check the available pores before each run

[@JohnnyMnemonic882]

Does it offer the code in full form of all DNA? Some chromosome? Or the sequence of some gene?

[@matanoren2220]

Depending what you sequence. This is the rapid protocol, so it will sequence parts of the target sequence (either a genome or a PCR product)