For anyone watching this 11 years later, there’s something I want to clarify about this video First off, the enzyme and substrate do not fit like lock and key. A better analogy would be a glove; the enzyme and substrate shapes slightly change in order to fit each other, but not too large extent. This means that what he said about enzymes and substrates not changing she is actually wrong. However, I guess that’s an easier way to understand it, but it may not be good for high school and higher
Sorry if this is a bit late but, H2SO4 stops the reaction because it's a strong acid. Because of this, it quickly breaks up into the ions H+ and SO4 2-. Now the H+ ions lower the pH of the solution and since the amino acids that comprise proteins are generally suited for neutral or slightly acidic/basic environments the folding of those amino acids into their native structures (secondary, tertiary, quaternary) are disrupted, and the protein, in this case catalase, in this case is denatured.
Is it possible to have a similar experiment to test the effect of substrate concentration? Can we dip the filter paper in different concentrations of hydrogen peroxide and drop them in a fixed concentration of yeast?
What about if there was more salt concentration added the same amount of yeast, same amount of normal hydrogen peroxide? You know like if IF MORE OR LESS salt concentration was added? Would it destroy the enzyme if more salt was added or if small amount of salt was too?
how would you calculate the uncertainty for reaction rate? would it just be the same as your uncertainty for time? or would you need to find the percentage uncertainty
does anyone happen to have a detailed lab protocol that they could share with me for this lab? we've done a different version of this lab but I like to yeast idea...thanks for the phenomenal videos, Paul!!
I know it has been a year, But I have a version of this lab that I am trying out for the first time. If it goes well, I can share it with you if you are still interested
Andwele Harris depends on west/east side of Ukraine; in eastern part lots of people can and do speak both: Ukrainian and Russian, in the west (especially in countryside) people usually speak only Ukrainian In my personal experience, it’s somehow possible to understand each other, like when I was at my relatives’ in Lviv, we used to just talk to each other both in our native languages)
You're a lifesaver! I genuinely thought I was going to fail this lab, and pull my B to a C or D. Thanks so much for the simple explanation 😊
thank god i found this the friday before the exam! i will watch EVERYTHING and pray to god that i pass! good luck to everyone taking the exam!
So... How'd the exam go?
@@Bkboy911 ur 10yrs to late for that bruh
lol i think its funny how all his endings are so abrupt
It's hard to find a good teacher.. This guy ROCKS!!!
It's 2016 but I still think his animations are great
2021 now and still great lol
2024 and its indeed still great
Anddddd this is my science fair project
aaanndd this is my IA
For anyone watching this 11 years later, there’s something I want to clarify about this video
First off, the enzyme and substrate do not fit like lock and key. A better analogy would be a glove; the enzyme and substrate shapes slightly change in order to fit each other, but not too large extent. This means that what he said about enzymes and substrates not changing she is actually wrong. However, I guess that’s an easier way to understand it, but it may not be good for high school and higher
Sorry if this is a bit late but, H2SO4 stops the reaction because it's a strong acid. Because of this, it quickly breaks up into the ions H+ and SO4 2-. Now the H+ ions lower the pH of the solution and since the amino acids that comprise proteins are generally suited for neutral or slightly acidic/basic environments the folding of those amino acids into their native structures (secondary, tertiary, quaternary) are disrupted, and the protein, in this case catalase, in this case is denatured.
Thanks for helping me pass college biology(: Keep making videos, please!!
Is it possible to have a similar experiment to test the effect of substrate concentration?
Can we dip the filter paper in different concentrations of hydrogen peroxide and drop them in a fixed concentration of yeast?
How much concentration of yeast did you use for each which one was the starting concentration and how did you increase
I wish he was my real teacher!!
8 years later and we still have bad teachers.
I'm studying the IB program, so I just wanted to ask if this is too simple. Isn't this more appropriate for middle school?
What about if there was more salt concentration added the same amount of yeast, same amount of normal hydrogen peroxide? You know like if IF MORE OR LESS salt concentration was added? Would it destroy the enzyme if more salt was added or if small amount of salt was too?
Thank you SO MUCH!!!! your videos helped me!! hope I pass tomorrow...>
why is it that the greater concentrations takes less time to float up
+samuel Tao More yeast,more catalase,more breakdown of H2O2, more H2O and O2, suitable for good floatation.
Cheers.
Thank you very much! This helped me a lot :)
how do you change the concentration of yeast ??
so is it competitive inhibitors that are overcome by the addition of more substrate?
Hey Mr.Andersen, i was wondering why the graph starts at .01 on the Y-Axis. Could you explain this to me?
how would you calculate the uncertainty for reaction rate? would it just be the same as your uncertainty for time? or would you need to find the percentage uncertainty
Hello how did you know what the molarity of yeast would be used
so help thank you
I letrally get excellence's and merits because of your wonderful teaching and I'm black :O
does anyone happen to have a detailed lab protocol that they could share with me for this lab? we've done a different version of this lab but I like to yeast idea...thanks for the phenomenal videos, Paul!!
I know it has been a year, But I have a version of this lab that I am trying out for the first time. If it goes well, I can share it with you if you are still interested
@@caitiec9688 I am very much interested!! Will you post it please?
what positive and negative controle
why does the protein get denatured at too high of temperatures?
Hello may I know the temperature of the yeast mixture
thank you!!
what happens if there is too much/litte catalase then the substrate (hydrogen peroxide)?
+farah ali the catalase will eventually break down all the substrate regardless of amount
It's not in russian, actually)
"Перекис водню" is in ukrainian.
In russian it's like "Перекись водорода".
they're quite similar wow.... do most Ukranians speak Russian?
Andwele Harris depends on west/east side of Ukraine; in eastern part lots of people can and do speak both: Ukrainian and Russian, in the west (especially in countryside) people usually speak only Ukrainian
In my personal experience, it’s somehow possible to understand each other, like when I was at my relatives’ in Lviv, we used to just talk to each other both in our native languages)
energy breaks chemical bonds, changing the shape of the protein. if you use his analogy of enzymes as keys, new lock shape, then old keys don't work.
Sir will u please tell how we calculate activity of catalase activity by using spectrophotometer.
ib is harder than ap but ap is considered college level- the actual difficulty is upon the individual (it should be easy for most id hope though)
why is H2SO4 used to stop the reaction?
Thank you sir it helps a lot :)
thanks
So independent, dependent, control, null hypothesis, interpretation
Likely, because low temperatures doesn't give enough energy to lose a connections inside a protein.
Read smth about "Activation energy".
it will break down the enzyme- its a strong acid and damages it
it does clean it hmph. it just destroys good bacteria too heheh
H🗿
More of high school like freshman but yea.
Heh, it's actually in Ukranian. Twice the WTF.