Colourimetric assays (aka colorimetric assays) What are they? and how do they work?

Technique: Colourimetric Assays
The LDH and Resazurin Assay
In this video I explain what colourimetric assays are and why they are useful. Then described the LDH and Resazurin Colourimetric assays.
Learning objectives
Describe what a colourimetric assay is and why they are usefull
Describe the steps of the LDH assay and explain what it is measuring
Describe the steps of the resazurin assay and explain what it is measuring
‣ Explain one advantage of the resazurin assay over the LDH assay
Explain how a spectrophotometer plate-reader works with examples
‣ Determine which coloured light should be used by the spectrophotometer based on the apparent colour of the well