How to prepare ammoniacal silver

Fresh solutions of ammoniacal silver are essential for histological applications, but require careful preparation and handling. This video provides step-by-step instructions for preparing a sufficient volume of good quality ammoniacal silver to stain approximately 50 slides. Techniques for safe disposal of left over solution are also covered.

[@JULISSALENKAMENDOZAAQUINO]

podria utilizar solo hidroxido se sodio ? es necesario que use amoniaco tambien ????

[@atitayaroumwong3469]

Thank you so much for this knowledge

[@damienharkin]

You are most welcome!

[@nidhalalshamary8749]

Can we prepare amonical silver nitrate by adding AgNo3 to amonium hydroxid?

[@damienharkin]

Possibly. I’ve come across a few different methods but have not tried this approach myself.

[@khanoniwilliams7889]

So you don’t have to filter?

[@damienharkin]

I've not routinely filtered my solutions of ammoniacal silver since I usually only prepare 10 mL at a time, but it is worth trying as a way to reduce background if this is a problem. Let me know how you go if performing a comparison.

[@HARJOTSINGH-dy5bs]

love u sir ji