How to Interpret Gel Electrophoresis Results: Different types of plasmid DNA

[@jahidhassan7775]

Very nice video ma'am. Ma'am how to know which one is plasmid DNA and which one is chromosomal DNA by seeing in gel electrophoresis?

[@dorfinhorg9458]

If you see only two bands on your gel, how can you tell what conformation that plasmid is in? For example, it could be supercoiled and linear, or it could be linear and nicked circular...any advice here?

[@katelar7576]

I guess you can digest it with restriction enzyme and have a separate well with it, so you could say for sure where linear version is.

[@graemelaubach3106]

Very helpful video, with great animations! Much appreciated :D

[@arindampaul5256]

Mam ethydiumbromide is added in gel or Sample plasmid?

[@Goldbio]

Thanks for your question. Generally, it is added in the gel. About 3 minutes into our video about gel electrophoresis, it talks about waiting for your gel to cool and then adding the ethidium bromide or equivalent: th-cam.com/video/tMyYgEQ2pf4/w-d-xo.html
:)

[@vazquezvalemtine]

God sent explanation, my lecutere literally sent us to look this up. Thanks

[@imanalansary4538]

Thanks for your explanations ❤

[@iashsch_]

Super useful👍

[@jacksonmulwa9406]

Exactly what I want

[@regina5613]

Super!

[@nadasalah8455]

Thanks alot❤❤❤❤❤❤❤❤❤❤❤❤❤❤❤❤❤❤❤❤❤