Preventing Objective Lens Damage: Immersion Oil Problems
ฝัง
- เผยแพร่เมื่อ 1 ส.ค. 2024
- This video describes the problems that can be caused by excessive immersion oil.
Video created by Jennifer Waters, Director of the Nikon Imaging Center at Harvard Medical School, with helpful suggestions from Talley Lambert.
Image of oil under top lens from:
Ross, Practical considerations of objective lenses for application in cell biology. doi: 10.1016/B978-0-12-420138-5.00002-1.
Image of oil under top lens from:
Petrak & Waters, A practical guide to microscope care and maintenance
DOI: 10.1016/B978-0-12-420138-5.00004-5 - วิทยาศาสตร์และเทคโนโลยี
Thank you very much for this video! It was very informative, and the use of actual videos of the different cases helps a lot!
Thank you for the upload, I hope you will continue to do so.
Waiting for the cleaning oil from objective video :)
Very useful information.
Looking to buy a nice (kinda cheap $600 microscope). It's expensive for an undergrad and I forever appreciate this guide.
Hello, thank you for the nice vid. I recently baught a microscope and find the mechanism that you speak of that allows the lense to move inwards when too much pressure is applied, to make a grinding sound when pushed in, much unlike the smooth movement your image shows. The objective is cheeper, however, can I apply light oil or some other method to smooth this out? Thanks!
Cool! But how do I get cedar tree oil off my dry lens? :(
Some further thoughts on cleaning would be appreciated. I've seen frequent cleaning with 100% EtOH or commercial glass cleaning solutions being recommended, although to me this seems to risk removing coatings on the top lens or penetrating cement. I understand that if left, immersion oils can become corrosive, but surely less so than hard solvents.
Check out this video on the topic: www.microlist.org/listing/cleaning-microscope-optics-youtube-video/
@@Microcourses I've watched it, it's pretty bad and comments are disabled.
For example, she clean the filter, then, use a manual a manual air blower if there is dust left.
That's pure incompetent here. You first blow the particules, then you touch the filter with solution if it's still required. Otherwise, you risk scratching the optic with the dust.
Always the least invasive to more aggressive cleaning.
The video link is not working, I've found it by searching on TH-cam.
Thank you for your video but i have a question, can water damage your objectives, I submerged the 40x into a water sample but i’m worried i’m doing something
wrong
If the lens is not designed to be submerged in water this may have damaged it. Contact the lens manufacturer to ask.
thx for replying
im having problems with my 40x objective, because im finding that i have to move it really close to the actuall slide and cover slip with the sample, it has lots of dirt, potential scratches and recently gott water in it because the cover slip broke from the objective being so close, is there any way to get detailed focus on a 40x objective without going to close to the slide or cover slip?
The answer is no. The focus point is the focus point. Having the thinnest samples possible will give your objective the most distance from the coverglass.
Learn proper sample preparation technique (mainly it should be very thin, 10 time smaller than a grain of salt. To break a cover slip, you must be outrageously bad at it!
If you are still clumsy, you can buy a long range 40x objective. It's very expensive and it will give you few mm more room.