Great video.. with extremely good lenses you can get nothing but empty magnification when you get higher than 1400x.. The only way to double that is to go UV
The resolution of a microscope is limited by something called the point spread function. Essentially a tiny particle smaller than the resolution limit will appear as a large and blurry light instead of the sharp particle.
Hello, I have a question, TL;DR: I'd like to have a greater magnification than the objective magnification when using a microscope camera (since there's no eyepiece, max is x100), is a barlow lens usable in that setup (inbetween a microscope camera and the trinocular tube?). I have swift 380t! Thanks. ______________
I've recently started using a microscope camera more frequently instead of looking through the eyepiece, as I find it more comfortable and also to take and share pictures more easily. When using the eyepiece, the final magnification is calculated as such: the objective magnification (x4 to x100) times the eyepiece magnification (I have x10 and x25). I've seen in some of your videos that you also could add x2 intermediate piece for even bigger magnification (even if pointless). Now, when I use the camera, there isn't a final eyepiece that "scales things up even more". So the maximum magnification I'm able to achieve, optically, is x100 (but I don't like using oil so I keep it at x60). I'd like to know how to achieve, on camera, more than x100 magnification. I've seen videos of Microcosmos where on camera he has x400 at times. I know it will not define the picture better, but it'll still be better to optically upscale images to see the details than to digitally upscale it I guess! So, what tool do I use to go from x60 to x400? Do I need to put multiple "intermediate pieces" that do like x2, going to x120 then x240? Or is there another solution? Thanks!
I guess you can use three 2x Barlow lenses which will make it go from 60x to 480x and reduce the field of view by 8 times. In theory. In which case it's probably better to just upgrade the normal eyepiece and capture directly from there with another camera. I don't know if that's viable actually. Let me know if you know a better way 😗
Thanks for all your advice n knowledge you sharred. I watched your vedios since 90 days . I started when i was trying to buy a new microscope and was so confused by the product s' descriptions . your vedios helped me a lot. after watching many of your vedios atlast i got the points and able to find a really decent microscope within my budget . its really good .. i will talk about it latter.. bt now i want to ask why i cant buy anything from your amazon store while i bought my microscope from amazon. I m from India Kolkata .I started microscopy about 60 days ago n really find it intresting . its a 5yrs old dream came true now.. 2nd question - What if we consider changing objective rather the eyepeice? i found nikon 150x eyepeice is available . and for the brand name i trust that its not a fraud.. so what if we change the objective with 150x and a eye peice of 20x ..it makes 3000x .. my question is what is better or they give same result 1.- 150x objective with 10x eyepeice / 2.- 100x objective with 15 x eyepeice??
Hello, 1. Watch this video on the combination of eyepiece and objectives: th-cam.com/video/HIDjksiwn6c/w-d-xo.html 2. The shop is only amazon.com. If you want to buy microscopes in India, directly visit amazon.in or go to www.microscopes-india.com/ All the best.
Wow so theoretically connecting 100 Barlow lenses with 25x eyepiece you can get magnification above 100.000.... So one be able to see the essence of all living and even 4th dimension. Great video btw!
Looks like 1500x is realistic with higher contrast specimens. Definitely more detail than 1000x. I think usable 3000x would be achievable with top quality lenses and deep purple filtered light.
a light microscope is better. digital usb microscopes (which look like a thick pen) are essentially cameras with a lens and can not magnify 1000x, even if it says that it is able to do that.
I'm trying to make a microscope from a bunch of lens from old phones/telescope lens/& laser lens...... How would i increase the magnification, would adding more lens increase it or 🤔?.. I want magnification to be really high like 500million+, i will be adding a light(probably xray type) later on to hopefully see atoms n all of that. Any advice?
The maximum useful magnification of light microscopes is 1000x. To see atoms, you need atomic force microscopes. Atoms are too small to reflect photons in a way that you can create an image.
Could there be a workaround? - use (polarized) light with very short and narrow wavelength(s) with CCD camera, focus stacking and automatic color assignment?
No, the quality is not the same. You should always have a high magnification objective and a lower magnification eyepiece. A high magnification eyepiece will result in a blurry image. The 2x2x25x combination corresponds to a 100x eyepiece. This is too much. The 40x objective can deliver more details (has a higher numerical aperture) than the 10x objective.
Great respect for you Oliver, but you can magnify an image as much as you want. But you cannot see any more detail above 1000x. So 6000x is total fantasy.
Ich mag deinen TH-cam Kanal ich möchte nett sein weil ich dich mag und ich deinen Tag besser machen will und positive Energie verbreiten will 👍mach weiter so
For compressed air one should not use commercial compressed air cans, because there are other substances in it. I once made a compressed air blower: th-cam.com/video/h1OhB2BCYHo/w-d-xo.html
Thanks a lot for video. 60x objective looks like decreasing too much image quality. I really want to see comparison 2x and 4x borlow lens with 40x objective.
Hi! Quick question. I would like to get my girlfriend a microscope as a gift. She is a Lab technician and wants a professional microscope to observe blood specimens and various pathologies. What is the maximum magnification necessary and is there any way to get a higher specific resolution in order to improve sharpness of images? What microscope do you recommend? By the way, i really enjoyed this video. It's exceptionally educational!! Thanks a million :)
TO show my utter ignorance of optics despite having had studied it for a long while, what if you made a mirrored concave slide and feed lite from the top could you get a better resolution?
There are top-illumination objective around, which have a separate light channel through which light is fed from the top to the specimen. I don't know much about these, though. I can imagine though, that if the specimen is on the mirror, it would reate a shadow on the slide and so, maybe the ligtht would not be able to reach the mirror to be reflected. I guess it would look like top-illumination. Generally, Abbes law states that the resolution depends on the angle of the light cone that the objective is able to catch, and if this light cone can not be increased, then resolution can not be increased. So you would have to move the objective closer to the specimen to do that.
There is a way to achieve better resolution that is somewhat like you say, its called solid immersion lens (SIL), its basically a very small hemispheric super-spheric or spherical magnifying glass, less than 2 mm in size but can be as small as few um that you put over the specimen and see thorough it with the microscope. The reason why its possible to achieve higher resolutions is that the lens has to be almost in contact with the specimen, at least half wavelength to couple with the near field of the specimen and the microlens must be of a very high refraction index, the higher the better, thus due the large angle of incidence and very high index of refraction this works like an objective of extremely high numerical aperture it can be as high as 3.3NA in the case of silicon lenses but those work only at infrared wavelengths, for visible wavelengths many materials with refraction index of 1.9 to 2.5 can be used like barium titanate glass, titanium dioxide, silicon carbide and many others, with this is possible to achieve a resolution of about 120-100nm but its quite tricky and only works well with relatively flat objects because as i mentioned before the lens has to practically be in contact with the specimen and also you need to use incident light so the same micro lens works as a very high NA condenser. There has been quite some research about this and mounting the micro lens on a special stage attached to the normal objective to scan a large area and then stitch a large image but still it seems there is no commercial product except on the microfab industry and its used to inspect microelectronics embedded in silicon wafers. Ive tried this using 50um barium titanate microspheres by sprinkling some with a toothpick over a DVD disk and then adding water so the focal point of the micro sphere is just outside it instead of inside (reason why a hemisphere is usually better) this enables you to see the lands and pits with very good clarity, much better than even using the oil immersion objective, best of all you can use a dry objective as low as 10x .25NA and still see the pits and lands, many papers show is even possible to do this with a blu-ray disk but i haven't had reproducible success, of all the attempts and thousands of microspheres i have looked at only about 1% actually show the grooves of the blu ray, i think must be my bad quality incident light setup as i improvised using a very small mirror on the objective to bounce back light from below and also i bough "cheap" microspheres so their index of refraction might vary significantly. Edit: the acronym is SIL not SIM
I would imagine they could use a 40x objective with 25x eyepieces, but usually inverted microscopes are better for live cells/specimens, and you would put oil on the objective itself in that case (I don't own a microscope yet, just been doing a bunch of research for one :) )
ehhrrr wouldn't the phone automatically focus based only on contrast and not luminosity? The ants leg at 100x was highest contrast, but at 6000x the dust was highest contrast... you should do this experiment using the DSLR and cam barlows
thank for the video ;) of course going over 1500x max is completely useless as it goes over the theoretical resolution. This barlow is only magnifying blur and diffraction from dust :) no more interestion details of the image. thanks for the demo as we can see the results.
Magnification without clear resolution is worthless. You don't use barlow lenses on a microscope to increase magnification. It's best to use 4 or 8K video with 60-125X digital zoom. I have solved one problem no one else has solved and that is how to increase magnification without losing depth of field. Hint--you can magnify INTO and object or magnify OUT of an object. Magnifying an object OUT almost completely eliminates the dept of field problem and you get perspective as if you were standing there shrunk down to the size of a dust mite. Oh you must think of the box you guys!
I got a cellphone holder and the lens was made of plastic and covered with imperfections that looked just like this.. no amount of rubbing alcohol helped, so maybe the Chinese optics he got are the same ¯\_(ツ)_/¯
@@theunseen010 yeah "nice" metal construction, with plastic lenses. Wouldn't be one bit surprised. If it is indeed plastic using chemicals like acetone could actually destroy the lenses and parts, so be careful what you clean with. (I know you said rubbing alcohol, but still).
What is the best way to achieve highest good quality magnification on a mid priced microscope? Can you achieve 1000x? On some videos you only recommend the 10x eyepiece and 60x objective. That gives "only" 600x in total.
![กิโลเมตรที่ 0 เดินทางรอบโลกไม่ใช้เครื่องบิน [Around The World Ep. 1]](http://i.ytimg.com/vi/toJG9oofLFY/mqdefault.jpg)
![BABYMONSTER - 'DRIP' M/V REACTION [WAS NOT EXPECTING THIS!!!]](http://i.ytimg.com/vi/t0Lpgr1jo3Y/mqdefault.jpg)
This is a quality youtube channel. Instead of just saying that the limit is 1000x, you actually went past it and showed us. Thank you
Great video.. with extremely good lenses you can get nothing but empty magnification when you get higher than 1400x.. The only way to double that is to go UV
Sure, but I would not look through the oculars with the naked eyes.
The resolution of a microscope is limited by something called the point spread function. Essentially a tiny particle smaller than the resolution limit will appear as a large and blurry light instead of the sharp particle.
Nope, it's limited by something called diffraction limit.
@@ciprianpopa1503 you two are both saying the same thing
@@Top-Code Nope, one is the phenomenon (diffraction) and the other thing (point spread) is the mathematical description of this phenomenon.
Thanks for the diversion. Hope you and yours are doing well in this time of uncertainty. Stay safe and sane.
Hello, I have a question, TL;DR: I'd like to have a greater magnification than the objective magnification when using a microscope camera (since there's no eyepiece, max is x100), is a barlow lens usable in that setup (inbetween a microscope camera and the trinocular tube?). I have swift 380t! Thanks.
______________
I've recently started using a microscope camera more frequently instead of looking through the eyepiece, as I find it more comfortable and also to take and share pictures more easily.
When using the eyepiece, the final magnification is calculated as such: the objective magnification (x4 to x100) times the eyepiece magnification (I have x10 and x25). I've seen in some of your videos that you also could add x2 intermediate piece for even bigger magnification (even if pointless).
Now, when I use the camera, there isn't a final eyepiece that "scales things up even more". So the maximum magnification I'm able to achieve, optically, is x100 (but I don't like using oil so I keep it at x60). I'd like to know how to achieve, on camera, more than x100 magnification. I've seen videos of Microcosmos where on camera he has x400 at times.
I know it will not define the picture better, but it'll still be better to optically upscale images to see the details than to digitally upscale it I guess!
So, what tool do I use to go from x60 to x400? Do I need to put multiple "intermediate pieces" that do like x2, going to x120 then x240? Or is there another solution? Thanks!
I guess you can use three 2x Barlow lenses which will make it go from 60x to 480x and reduce the field of view by 8 times. In theory. In which case it's probably better to just upgrade the normal eyepiece and capture directly from there with another camera. I don't know if that's viable actually. Let me know if you know a better way 😗
Did you find an answer to your question? I am having the same dilemma. I don’t know why this very important question has no straight forward answer!
You can overlay images with each rotation to make the dust be dismissed.
Good tip! I would prefer the lenses to be clean, though.
Thanks for all your advice n knowledge you sharred. I watched your vedios since 90 days . I started when i was trying to buy a new microscope and was so confused by the product s' descriptions . your vedios helped me a lot. after watching many of your vedios atlast i got the points and able to find a really decent microscope within my budget . its really good .. i will talk about it latter.. bt now i want to ask why i cant buy anything from your amazon store while i bought my microscope from amazon. I m from India Kolkata .I started microscopy about 60 days ago n really find it intresting . its a 5yrs old dream came true now.. 2nd question - What if we consider changing objective rather the eyepeice? i found nikon 150x eyepeice is available . and for the brand name i trust that its not a fraud.. so what if we change the objective with 150x and a eye peice of 20x ..it makes 3000x .. my question is what is better or they give same result 1.- 150x objective with 10x eyepeice / 2.- 100x objective with 15 x eyepeice??
Hello,
1. Watch this video on the combination of eyepiece and objectives: th-cam.com/video/HIDjksiwn6c/w-d-xo.html
2. The shop is only amazon.com. If you want to buy microscopes in India, directly visit amazon.in or go to www.microscopes-india.com/
All the best.
Vedios???🤔
What microscope did you buy? I am looking also to get one!
I had a dream that I had microscope and looked like electron microscope and I was happy I'd love to have 6000x magnification too.
What's about camera does it get 6000x too ?
Wow so theoretically connecting 100 Barlow lenses with 25x eyepiece you can get magnification above 100.000.... So one be able to see the essence of all living and even 4th dimension. Great video btw!
Great video! Always informative!👍😀
Looks like 1500x is realistic with higher contrast specimens. Definitely more detail than 1000x. I think usable 3000x would be achievable with top quality lenses and deep purple filtered light.
Should I buy a digital microscope with a range of 40x-1000x. Or a light microscope with 3 lens of 400,800,1200x.?
a light microscope is better. digital usb microscopes (which look like a thick pen) are essentially cameras with a lens and can not magnify 1000x, even if it says that it is able to do that.
@@MicrobehunterMicroscopy ok got it , thanks ... And I really appreciate your work , your reviews regarding the microscopes helps alot .
I'm trying to make a microscope from a bunch of lens from old phones/telescope lens/& laser lens...... How would i increase the magnification, would adding more lens increase it or 🤔?.. I want magnification to be really high like 500million+, i will be adding a light(probably xray type) later on to hopefully see atoms n all of that. Any advice?
The maximum useful magnification of light microscopes is 1000x. To see atoms, you need atomic force microscopes. Atoms are too small to reflect photons in a way that you can create an image.
@@MicrobehunterMicroscopy any videos on how to create that or how it works on your channel I can check out?
This has nothing to do with the magnification but I see those weird “worm” like things floating in my right eye and thought it was acting up lol 😂
Could there be a workaround? - use (polarized) light with very short and narrow wavelength(s) with CCD camera, focus stacking and automatic color assignment?
Using short wavelengths is possible, but there is still a limit to resolution that will not allow for much more magnification.
U have to shine light from the upper portion of slides
Or just use laser and a polariser
If you were to use the 2x2x barlow lens *10x objective *25x eye lens, would the resultant 1000x be as sharp as the 40x objective*25x eye lens?
No, the quality is not the same. You should always have a high magnification objective and a lower magnification eyepiece. A high magnification eyepiece will result in a blurry image. The 2x2x25x combination corresponds to a 100x eyepiece. This is too much. The 40x objective can deliver more details (has a higher numerical aperture) than the 10x objective.
Great respect for you Oliver, but you can magnify an image as much as you want. But you cannot see any more detail above 1000x. So 6000x is total fantasy.
I've noticed that a similar Olympus microscope was used by Will Smith in the movie "Concussion"
oh my you look very much like one of my high school and university classmates
Ich mag deinen TH-cam Kanal ich möchte nett sein weil ich dich mag und ich deinen Tag besser machen will und positive Energie verbreiten will 👍mach weiter so
I think the dust is actually the imperfection of the lens glass.
Is there no way to remove the dust with a lens pen or compressed air? Also, can you increase the illumination of the specimen to compensate ?
For compressed air one should not use commercial compressed air cans, because there are other substances in it. I once made a compressed air blower: th-cam.com/video/h1OhB2BCYHo/w-d-xo.html
Microbehunter Microscopy thank you
Thanks a lot for video. 60x objective looks like decreasing too much image quality. I really want to see comparison 2x and 4x borlow lens with 40x objective.
Hi! Quick question. I would like to get my girlfriend a microscope as a gift. She is a Lab technician and wants a professional microscope to observe blood specimens and various pathologies. What is the maximum magnification necessary and is there any way to get a higher specific resolution in order to improve sharpness of images? What microscope do you recommend?
By the way, i really enjoyed this video. It's exceptionally educational!! Thanks a million :)
Oh dang he never responded:(
@@phi1688 darn
Did you end up getting her a microscope? If so which one did you end up getting her?
I love this kind of fun click bait, Stack those lenses.
Eyepiece 1x...objective 1000x 😁😁😁😁😁
Good education
TO show my utter ignorance of optics despite having had studied it for a long while, what if you made a mirrored concave slide and feed lite from the top could you get a better resolution?
There are top-illumination objective around, which have a separate light channel through which light is fed from the top to the specimen. I don't know much about these, though. I can imagine though, that if the specimen is on the mirror, it would reate a shadow on the slide and so, maybe the ligtht would not be able to reach the mirror to be reflected. I guess it would look like top-illumination. Generally, Abbes law states that the resolution depends on the angle of the light cone that the objective is able to catch, and if this light cone can not be increased, then resolution can not be increased. So you would have to move the objective closer to the specimen to do that.
There is a way to achieve better resolution that is somewhat like you say, its called solid immersion lens (SIL), its basically a very small hemispheric super-spheric or spherical magnifying glass, less than 2 mm in size but can be as small as few um that you put over the specimen and see thorough it with the microscope. The reason why its possible to achieve higher resolutions is that the lens has to be almost in contact with the specimen, at least half wavelength to couple with the near field of the specimen and the microlens must be of a very high refraction index, the higher the better, thus due the large angle of incidence and very high index of refraction this works like an objective of extremely high numerical aperture it can be as high as 3.3NA in the case of silicon lenses but those work only at infrared wavelengths, for visible wavelengths many materials with refraction index of 1.9 to 2.5 can be used like barium titanate glass, titanium dioxide, silicon carbide and many others, with this is possible to achieve a resolution of about 120-100nm but its quite tricky and only works well with relatively flat objects because as i mentioned before the lens has to practically be in contact with the specimen and also you need to use incident light so the same micro lens works as a very high NA condenser. There has been quite some research about this and mounting the micro lens on a special stage attached to the normal objective to scan a large area and then stitch a large image but still it seems there is no commercial product except on the microfab industry and its used to inspect microelectronics embedded in silicon wafers.
Ive tried this using 50um barium titanate microspheres by sprinkling some with a toothpick over a DVD disk and then adding water so the focal point of the micro sphere is just outside it instead of inside (reason why a hemisphere is usually better) this enables you to see the lands and pits with very good clarity, much better than even using the oil immersion objective, best of all you can use a dry objective as low as 10x .25NA and still see the pits and lands, many papers show is even possible to do this with a blu-ray disk but i haven't had reproducible success, of all the attempts and thousands of microspheres i have looked at only about 1% actually show the grooves of the blu ray, i think must be my bad quality incident light setup as i improvised using a very small mirror on the objective to bounce back light from below and also i bough "cheap" microspheres so their index of refraction might vary significantly.
Edit: the acronym is SIL not SIM
i have seen footage of 1000x magnification on live specimens. how do people do that since the 100x objectives require oil?
I would imagine they could use a 40x objective with 25x eyepieces, but usually inverted microscopes are better for live cells/specimens, and you would put oil on the objective itself in that case (I don't own a microscope yet, just been doing a bunch of research for one :) )
Thanks!
But one question. Why then invest in objectives if eyepieces are so much cheaper?
Eyepieces make the picture bigger but don't increase the resolution. Watch his other videos.
ehhrrr wouldn't the phone automatically focus based only on contrast and not luminosity? The ants leg at 100x was highest contrast, but at 6000x the dust was highest contrast... you should do this experiment using the DSLR and cam barlows
He could have set the focus manually, he was doing this just to show that such high magnifications don't work for light microscopy
thank for the video ;) of course going over 1500x max is completely useless as it goes over the theoretical resolution. This barlow is only magnifying blur and diffraction from dust :) no more interestion details of the image. thanks for the demo as we can see the results.
So pretty much any Light Microscope over 1500x is a scam? Or is there some new technology?
@@cantthinkofnameyeah7249 late but yes. all light microscopes over 1500x are trash
I wanna test this
the footage at 6000x still isn't bad though!
Thank you *sir*
Fuck quality image.... XDD
Magnification without clear resolution is worthless. You don't use barlow lenses on a microscope to increase magnification. It's best to use 4 or 8K video with 60-125X digital zoom. I have solved one problem no one else has solved and that is how to increase magnification without losing depth of field. Hint--you can magnify INTO and object or magnify OUT of an object. Magnifying an object OUT almost completely eliminates the dept of field problem and you get perspective as if you were standing there shrunk down to the size of a dust mite. Oh you must think of the box you guys!
the chinese says magnifying lenses
Para quem gosta de ver um borão em vez de bacterias, está otimo, pelas imagens está claro que passou de 1000x de aumento, as imagens são horriveis.
get some compressed air, great for blowing out that dust
Abbe is turning in his grave.
You can clean the Barlow Lens once. And re-upload the video .
I was thinking of dust proof camera with a air filter
I got a cellphone holder and the lens was made of plastic and covered with imperfections that looked just like this.. no amount of rubbing alcohol helped, so maybe the Chinese optics he got are the same ¯\_(ツ)_/¯
@@theunseen010 yeah "nice" metal construction, with plastic lenses. Wouldn't be one bit surprised. If it is indeed plastic using chemicals like acetone could actually destroy the lenses and parts, so be careful what you clean with. (I know you said rubbing alcohol, but still).
What is the best way to achieve highest good quality magnification on a mid priced microscope?
Can you achieve 1000x? On some videos you only recommend the 10x eyepiece and 60x objective. That gives "only" 600x in total.
There are also 100x oil objectives. Or the most simple way is just use 25x eyepiece.
Use your phone for more zooming
6000x magnification and no details. Cool 👎