Hello, thanks. I think that is a great suggestion considering when the filaments are attached or appear merged. I believe this macro will not segment and the way out is to manually segment (th-cam.com/video/ZHYIWPVlpzQ/w-d-xo.html) it before running the macro.
Hiii! whenever I use Fiji to analyze the particles in my image, I set it to exclude images that are on the edges, but it still considers them. Has the same thing happened to you? Could you help me?
@@nrttaye4033 Hiiii, thanks for answering me :D I was messing around with Fiji and ended up finding the setting described below: image> adjust> auto crop (guess background color) I put it in macro and it worked, thank goodness. anyway, thanks for the guidance, you always help me. all the best!
Perhaps it would be good if you include their segmentation also
Hello, thanks. I think that is a great suggestion considering when the filaments are attached or appear merged. I believe this macro will not segment and the way out is to manually segment (th-cam.com/video/ZHYIWPVlpzQ/w-d-xo.html) it before running the macro.
@@nrttaye4033 I guess this is important when you need to do single cell analysis and needcto relate them to cell
Hiii! whenever I use Fiji to analyze the particles in my image, I set it to exclude images that are on the edges, but it still considers them. Has the same thing happened to you? Could you help me?
Hi thanks for reaching out. it did not happen to me so far. Can you send me a sample image? I will try to troubleshoot it.
@@nrttaye4033 Hiiii, thanks for answering me :D
I was messing around with Fiji and ended up finding the setting described below:
image> adjust> auto crop (guess background color)
I put it in macro and it worked, thank goodness.
anyway, thanks for the guidance, you always help me.
all the best!
You figured it out, Excellent! All the best with your analysis.
how about if image is a z stack image
Hello, as far as i know this macro does not work for z stack.