Do you have a video where you show how to count cells in an invasion assay? Like how to compare surface cells to invaded cells and how to tell them apart?
The BD protocol specifies to removed the membrane from the insert with a sharp scape. Does anyone use this technique before counting the invaded cells?
Nope, because these are adherent cells so they stick to the chemically treated plastic chamber . We also use PBS to wash the debris off culture flasks when changing complete media.
Excuse me, but what's the difference between this assay and the migration assay? I see they're the same except for the Matrigel step here.. Another question; what is the function of the matrigel in this assay? Thanks.
Matrigel is what the cells migrate through. It mimics a basement membrane. The migration assay measures migration across a surface- a pipette is usually used to clear an area of cells. The Invasion assays measure cell movement through a medium (matrigel on a filter insert).
Matrigel and time are two factors that differs between migration and invasion. You don't need matrigel for migration essay, and you can evaluate it at 12 or 13 hours. Invasion needs matrigel because it mimics ECM with BM elements, just as an invasive cell. And it takes around 22 to 24 hours.
Do you have a video where you show how to count cells in an invasion assay? Like how to compare surface cells to invaded cells and how to tell them apart?
What is the dilution of Matrigel? I could see the same question asked by MG.
Do you touch to the membrane when you scraping off with cotton swaps?
i am gonna do this experiment....it's really helpful for me....thanks so much
Why do you count invasive cells in the upper chamber? It should be counted in the lower chamber, right?
what is matrigel dilute ratio?
congratulations on the work, I would like to know which matrigel was used for the experiment and which was the dilution of the matrigel
instablaster.
What is that lower chamber?
Can v see tube formation using this chamber? I mean angiogenesis assay
How much % of giemsa that used in this video ? thank you so much
The BD protocol specifies to removed the membrane from the insert with a sharp scape. Does anyone use this technique before counting the invaded cells?
Wash the chamber by PBS before fixing cells...
Won't the cells on the membrane be washed out ?
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哈哈…不會無趣啦
了不起! 祝你成功!
Nope, because these are adherent cells so they stick to the chemically treated plastic chamber . We also use PBS to wash the debris off culture flasks when changing complete media.
then you mean if I use suspension cells for same experiment, then cells will be washed out? then what should I have to do with suspension cells?...
Excuse me, but what's the difference between this assay and the migration assay? I see they're the same except for the Matrigel step here.. Another question; what is the function of the matrigel in this assay? Thanks.
Matrigel is what the cells migrate through. It mimics a basement membrane. The migration assay measures migration across a surface- a pipette is usually used to clear an area of cells. The Invasion assays measure cell movement through a medium (matrigel on a filter insert).
Matrigel and time are two factors that differs between migration and invasion. You don't need matrigel for migration essay, and you can evaluate it at 12 or 13 hours. Invasion needs matrigel because it mimics ECM with BM elements, just as an invasive cell. And it takes around 22 to 24 hours.
Cheer~~~determine the content or quality of (a metal or ore).
谢谢。
matrigel invasion assay