Great video! In the case of testing using direct IF for a disease such as Pemphigus Vulgaris, what is the antigen & what is the antibody? Which one is taken from the human sample?
It is an autoimmune disease. Antigen- Desmosome (desmoglein 3 protein) Antibody- IgG in serum(suspected). Confirm by looking for fishnet/ chicken wire pattern
Why to use blocking solution before the primary antibodies? Is there not a chance you will be blocking the protein you want to find? Or it is just worth it, because even if you block some of the target proteins, the primary antibody will just do better in finding the not blocked target proteins, than finding inespecific conections?
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
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hi, bro, what protocol are u using? can u name pleeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeassssssssssssssssssssssssssseeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeee
@@animatedbiologywitharpan sir u really give optimised protocol .. I am a first year phd student and interested in protocol which u r providing..can u give complete detail of the same
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8& You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
Sir.. We students are in serious trouble... Mh set exam conducted by SPPU university some days before for asst. Prof. In Maharashtra and goa... They published intrim answer key two days before... And there are 5-6 question are such that we can challenge them... So we want u to make video on that 6 question... Bcz when we challenge a question we have to give correct explaination from std books.Plzz sir help us..give me ur whtspp nmbr or email id so i can send u those question
Great video! In the case of testing using direct IF for a disease such as Pemphigus Vulgaris, what is the antigen & what is the antibody? Which one is taken from the human sample?
It is an autoimmune disease. Antigen- Desmosome (desmoglein 3 protein) Antibody- IgG in serum(suspected). Confirm by looking for fishnet/ chicken wire pattern
Why to use blocking solution before the primary antibodies? Is there not a chance you will be blocking the protein you want to find? Or it is just worth it, because even if you block some of the target proteins, the primary antibody will just do better in finding the not blocked target proteins, than finding inespecific conections?
It's like putting some obstacles, only desperate interaction will happen despite of the odds.
Very well explained.
Fantastic effort to explain such a difficult yet important topic.
👏🏻👏🏻
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@@animatedbiologywitharpan sure 🙏
Good presentation
animatedbiologywitharpan.graphy.com/courses/CSIR-NET-Life-sciences-MCQ-practice-course-645881d4e4b0c11d957360ef here you can find practice questions
Thank you, immensely benefited
Please share my channel link with your friends and help me to reach big audiance . namaste 🙏
You gotta be the best one to explain this technique
Please share my channel link with your friends and help me to reach big audiance
I❤ conjugate Ab
It saves a lot of time but it's expensive
awesome bro
please share my channel link with your friends and help me to reach big audience
thanks bro
Please share my channel link with your friends.
Much appreciated
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Nice
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Just wow💯
In order to make high quality content consistently we need support from you. Please support us by using super thanks.
Small contribution like Rs50/100 means a lot for us.
super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
hi, bro, what protocol are u using?
can u name pleeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeassssssssssssssssssssssssssseeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeee
I use a optimized protocol in the lab. In case you are interested i can give you training on protocol over zoom call @ $2 /hrs
@@animatedbiologywitharpan sir u really give optimised protocol ..
I am a first year phd student and interested in protocol which u r providing..can u give complete detail of the same
@@poornimasharma6632 eamil us at arpanparichha1994@gmail.com.
Bestest video
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8&
You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
bhaiya talk in hindi also for more understanding thank you
No.
Don’t mix English and Hindi in one video.
Then international viewers will not appreciate.
Sir.. We students are in serious trouble... Mh set exam conducted by SPPU university some days before for asst. Prof. In Maharashtra and goa...
They published intrim answer key two days before... And there are 5-6 question are such that we can challenge them... So we want u to make video on that 6 question... Bcz when we challenge a question we have to give correct explaination from std books.Plzz sir help us..give me ur whtspp nmbr or email id so i can send u those question