How can it be that none of the background light is entering the objective lens and only the light of the specimen is entering. There is some chance that the background light also enters the objective lens? For this, the specimen will have to be highly aligned with the objective lens and with light. According to this, we cannot align the bacteria in the dark field microscope, meaning we cannot see the bacteria, if there is a very big specimen, we can only see it in the dark field microscope. Am I understanding correctly Sir? Please reply sir
In dark-field microscopy, the background appears dark because the illumination is designed to bypass the objective lens unless it is scattered by the specimen. Only the light scattered by the specimen enters the objective lens, making the specimen visible against a dark background. Here's how this works: 1. Illumination Angle: The light source is angled so that the direct light misses the objective lens. This creates the dark field. 2. Specimen Scattering: The specimen scatters the light into the objective lens. Smaller objects (like bacteria) can scatter light effectively, so they appear bright. 3. Alignment: The system doesn't require "perfect" alignment of the specimen. As long as the light scattered by the specimen reaches the objective lens, it will be visible. Now, addressing the concerns in the text: Background Light: The optical setup ensures that no direct background light enters the objective lens. Small Specimens (e.g., bacteria): Dark-field microscopy is particularly good for small specimens because they scatter light efficiently. Large Specimens: Larger specimens might scatter too much light, which could potentially overexpose the field, but they can still be observed. Your interpretation that "we cannot see bacteria in a dark-field microscope" is not entirely correct. Bacteria are often better visualized in dark-field microscopy due to their size and ability to scatter light.
we know ,,making fool on the name of cancellation
Technically it's canceled for December
Already many people told its in feb 1 St week.
To bring out attension to studnets such words sir uses
@@shomusbiologyofficial yeah it's called postponing the exam and not the cancellation of exam... shameful
Sir,I have covered 7 units and now practicing pyqs..is this much enough to get jrf ?
Sir can I add new unit at this time ?
Yes
sir form bhrne me life science biology subject show nhi kr rha. h mera form fill nhi ho gya mene msc botany kiya hua h😢
Csir net form abhi release hi nehi hua. You were checking ugc net form
Atleast July 2024 how many members select jrf and overall how many jrf including 5 subject
Calculator is allowed in this exam?
Digital calculator
You will get it in your computer screen (digital calculator) they dont allow you to take physical calculator .
Sir ye to sure hai ab ki exam 19 jan tak ugc ke sath to nai hoga?????? Then 22 jan se 31 jan jee mains??? Then sir exam will be acc to you?????
Jan 12-13 or weekdays on 3rd week of Jan
How many jrf seats in life sciences how many members qulify jrf every six months
Varies. No fixed value
Sir biochem. Me bhut problem ho ri h plzzzzz btaiye kaise pdu kya krru bcz question b ni lgre usse 😢😢plzz suggest something
Watch my guide videos on biochemistry unit
This is good, more time for revision and practice
You're welcome
Sir, I've completed my bachelors and not enrolled in any masters programme. can I fill Csir Net form this time?
No
Yes u can apply...Maine apply Kiya tha graduation ke baad
You can apply with wrong data regarding your masters but of what use even if you qualified the exam ?@@kashishyadav5616
@@shomusbiologyofficial thanks sir
@@kashishyadav5616 how?
Thanks for the information bro
You're welcome
Sir, do we use immersion oil in a dark field microscope ?????
No
sir i am pursuing msc botany 2 year how should i crack jrf in first attempt i am expecting some tips from your channels will u please
Multiple videos are there in this channel regarding this topic. Please refer those
Sir u are making everyone fool or what for views its in jan last week or feb 1 st week again u repeated same thing for views.
Do you think I care about views?? Nobody will continue TH-cam for 12 - 14 years for organic views. I stated same thing that I stated earlier.
@shomusbiologyofficial exam is in jan last week or Feb 1 st week it was told earlier by so many again u are telling dec 2024 cancelled
How many jrf seats selected every six months
@@ChinthakayalaMeghana-r7lIn life science nearly 900-1100
I want help for csir net in mathematical science
This channel is for biological sciences
SAY POSTPONED 😒😑
There was a question mark in the thumbnail
Ek question mark laga k sari responsibility khatm ho gyi aapki.... Shomu sir, be a minimum sensible man... :-)
Thank you so much sir 🙏 for information
You're welcome
Sir please help! I am filling the form but it’s not showing life sciences in the subject opting window. What should I do?
@@Makima_saan-mt4ns csir(for science stream)net ke forms avi release nhi hue h
Because csir net form is not out yet. You were filling ugc net form
How can it be that none of the background light is entering the objective lens and only the light of the specimen is entering. There is some chance that the background light also enters the objective lens?
For this, the specimen will have to be highly aligned with the objective lens and with light.
According to this, we cannot align the bacteria in the dark field microscope, meaning we cannot see the bacteria, if there is a very big specimen, we can only see it in the dark field microscope.
Am I understanding correctly Sir?
Please reply sir
In dark-field microscopy, the background appears dark because the illumination is designed to bypass the objective lens unless it is scattered by the specimen. Only the light scattered by the specimen enters the objective lens, making the specimen visible against a dark background. Here's how this works:
1. Illumination Angle: The light source is angled so that the direct light misses the objective lens. This creates the dark field.
2. Specimen Scattering: The specimen scatters the light into the objective lens. Smaller objects (like bacteria) can scatter light effectively, so they appear bright.
3. Alignment: The system doesn't require "perfect" alignment of the specimen. As long as the light scattered by the specimen reaches the objective lens, it will be visible.
Now, addressing the concerns in the text:
Background Light: The optical setup ensures that no direct background light enters the objective lens.
Small Specimens (e.g., bacteria): Dark-field microscopy is particularly good for small specimens because they scatter light efficiently.
Large Specimens: Larger specimens might scatter too much light, which could potentially overexpose the field, but they can still be observed.
Your interpretation that "we cannot see bacteria in a dark-field microscope" is not entirely correct. Bacteria are often better visualized in dark-field microscopy due to their size and ability to scatter light.