I just wanna say, I’ve watched all these videos this morning prior to my micro lab prac and I had to double check the post dates because YOU LOOK GREAT! Congrats, I know that didn’t happen on accident!!!!
Holy crap thank you so much! I have a micro test tomorrow over all these tests and was so confused over the oxidase test. This cleared it up extremely well. Thanks so much!
That is more or less correct, I think. Check out this paper. The last few sentences of the abstract summarize the other types of cytochrome oxidases in oxidase-negative bacteria. It’s from 1986 though, so I don’t know if it is still fully accurate. www.microbiologyresearch.org/docserver/fulltext/ijsem/36/1/ijs-36-1-38.pdf?expires=1601420261&id=id&accname=guest&checksum=E73746693AE435AD424F8B4A558B4064
@@BiologyProfessor That paper adds more questions than answers ! Mammalian cytochrome c oxidase (a , a3) is present in the majority of the bacillus strains testing -ve on the oxidase test . So the (TMPD) oxidation might be associated with another set of oxidative enzymes ; NOT necessarily those of the aerobic respiration .
@@BiologyProfessor According to what we got from that paper ; the (TMPD) acts as an electron donor to cytochrome c in order to let that cytochrome proceed towards the final step of the respiratory chain catalyzed by the cytochrome c oxidase enzyme . In a way the (TMPD) simulates the role of reduced coenzyme Q (CoQH2) in complex III of mammalian respiratory chain . Lord knows best !
I just wanna say, I’ve watched all these videos this morning prior to my micro lab prac and I had to double check the post dates because YOU LOOK GREAT! Congrats, I know that didn’t happen on accident!!!!
Holy crap thank you so much! I have a micro test tomorrow over all these tests and was so confused over the oxidase test. This cleared it up extremely well. Thanks so much!
I hope your test went well!
Thank you so much for this online resource!
Thank you so much for making this video! I kept getting confused between the enzyme and the reagent. This video totally cleared it up for me. 😀
Clever presentation! Clear and precise! Thank you :)
Thanks for making a precise and accurate video. great teaching :)
You are a good teacher
clear !! micro exam tomorrow, thank u ....
Beautiful and Intellegent..Tysm
Nice explanation mam ☺️
Great Job ...!! ❤❤
Thank you!
Super explanation mam👌
I have viva tomorrow.. thankyou ❤
Good stuff
Congratulations for your channel I think is amazing. Quick question not from this video. Did you make excersise during the Keto diet ?
Yes, running and weights
Thank you professor:)
You are welcome!
shouldn’t it (cytochrome c oxidase)supposed to donates electrons to final electron acceptor oxygen
you've lost so much weight, you look really pretty!
Yeah!
I thought that was her sister but later I got know that it's the same person who is actually teaching us biology.
Thank u
Do the aerobic bacteria testing -ve on the oxidase test lack the cytochrome c oxidase ?
Yes, they often have another kind of oxidase to allow electron transport for aerobic respiration
@@BiologyProfessor
So the (TMPD) reagent is only oxidized by the cytochrome c oxidase NOT the other alternative oxidases .
That is more or less correct, I think. Check out this paper. The last few sentences of the abstract summarize the other types of cytochrome oxidases in oxidase-negative bacteria. It’s from 1986 though, so I don’t know if it is still fully accurate. www.microbiologyresearch.org/docserver/fulltext/ijsem/36/1/ijs-36-1-38.pdf?expires=1601420261&id=id&accname=guest&checksum=E73746693AE435AD424F8B4A558B4064
@@BiologyProfessor
That paper adds more questions than answers !
Mammalian cytochrome c oxidase (a , a3) is present in the majority of the bacillus strains testing -ve on the oxidase test .
So the (TMPD) oxidation might be associated with another set of oxidative enzymes ; NOT necessarily those of the aerobic respiration .
Good papers usually do that. ;) Sorry, I don’t know much more about alternative oxidases.
if I am performing both a catalase and a oxidase test to determine an unknown bacteria , which one would i do first ?
Catalase test
The catalase test is fastest, so I would do that first.
your lectures are awesome and u are beautiful too 😍😍😍🥰
Nice
Thanks for watching :)
love it
Do you know the chemical reaction of this test?
More info here: asm.org/getattachment/00ce8639-8e76-4acb-8591-0f7b22a347c6/oxidase-test-protocol-3229.pdf
@@BiologyProfessor
According to what we got from that paper ; the (TMPD) acts as an electron donor to cytochrome c in order to let that cytochrome proceed towards the final step of the respiratory chain catalyzed by the cytochrome c oxidase enzyme .
In a way the (TMPD) simulates the role of reduced coenzyme Q (CoQH2) in complex III of mammalian respiratory chain .
Lord knows best !
ｔｈａｎｋｓ ，ｖｅｒｙ ｃｌｅａｒ ｓｐｅｅｃｈ