Tasting Resiniferatoxin (RTX): The Spiciest Substance in the World | Full Extraction from Euphorbia

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  • เผยแพร่เมื่อ 20 พ.ค. 2024
  • Did you like this video? If so, be sure to check out this video, where I take on thioacetone: the smelliest chemical on Earth! Don't miss out, it's another TH-cam-first!
    • Making Thioacetone: Th...
    When people want to convey how spicy something is, they usually do so with the Scoville scale. A jalapeno is, for example, a bit less than a 10,000 on this scale, while a Carolina Reaper is around 2,000,000. So what sits at the top of the Scoville scale in the year 2022?
    Resiniferatoxin (RTX) is a white, waxy solid with a score of 16 BILLION on the Scoville scale. This substance is certifiably toxic, irritating, and corrosive in its pure form, and one of its only natural source is a poisonous, cactus-like plant native to Morocco. So...what happens if we TASTE it? According to the Internet, nobody has been mad enough to attempt this yet, so I'm stepping up to fill in the gaps! In this video, you will learn just about everything we know about this elusive substance, and even get the chance to watch me taste a small sample.
    Want to help my channel grow? Consider donating or becoming a channel patron:
    www.paypal.com/paypalme/labcoatz
    / labcoatz
    0:00 Intro
    0:53 Backstory
    3:41 Procedure
    5:11 Slight issue
    6:09 Procedure (continued)
    8:25 Vanillin-sulfuric acid stain test
    9:27 Tasting: Habanero pepper (capsaicin)
    11:27 Tasting: Resiniferatoxin
    13:17 HUH??
    13:39 Explanation
    15:53 Scoville scale demystified
    16:34 Conclusion
    17:18 Final remarks (and GIVEAWAY!)
    18:07 Patreon thanks and outro
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ความคิดเห็น • 1K

  • @LabCoatz_Science
    @LabCoatz_Science  ปีที่แล้ว +353

    Alright, could we all just take a moment to appreciate how much of that habanero I managed to eat?
    Also, quick note: I know the stuff I tasted wasn't pure and that the vanillin stain test wasn't highly conclusive. The plant I used was dying and kind of on the weak end (the latex didn't really irritate my skin, but it did taste spicy), so there wasn't a lot of RTX to begin with. Plenty of the resiniferatoxin was probably also lost to thermal decomposition and oxidation. But make no mistake: I did taste the resiniferatoxin in my sample. It was quite hot, despite my somewhat calm reaction, and there are no other compounds in resin spurge that create the burning sensation that I felt (irritation, yes, but not burning). Hope that clears up some of the controversy!

    • @Painandmisery6860
      @Painandmisery6860 ปีที่แล้ว +3

      Is it cuss if u eat enough it kills u???

    • @SetOfAllSets
      @SetOfAllSets ปีที่แล้ว +12

      if no one's ever tasted it how does it have a scoville rating?

    • @LabCoatz_Science
      @LabCoatz_Science  ปีที่แล้ว +24

      @@SetOfAllSets People have tasted it (it is found in euphorbium, which is an old medicine), I'm just the first person to show/taste it on video. I'm not sure if they used taste to determine the Scoville rating for RTX though...they might have just applied it to mice (or humans) and monitored how little it took to get a TRPV1 reaction.

    • @tomvolf
      @tomvolf ปีที่แล้ว +14

      @@LabCoatz_Science Ach, yes, of course, we do have Euphorbium officinarum as homeopathic remedy. So it is event current medicine, but in homeopathy potency.
      And I had to laugh ... you chemists are weird people. Yo (NileRed) says "is smells bad, but it is OK" and people around him are vomiting and fainting. And you can with nice smile eat the whole habanero (it would kill me instantly) and explain "it is hot, but just for short time period...".
      Anyway, thank you for explaining, how the Scoville rating is created, it was mystery for me.

    • @1337fraggzb00N
      @1337fraggzb00N ปีที่แล้ว +9

      Next episode: blood of a Xenomorph.

  • @WhiteTrashRenegade13
    @WhiteTrashRenegade13 ปีที่แล้ว +320

    This is probably not going to get read but I got some of this in my eye! I have a 30 foot euphorbia cactus in my front yard and I was cutting a piece up for my friend when some of the juice squirted in my eye! I was blind for a month while I was having dead epithelial tissue scraped off twice a week! Hurt like crazy this stuff is not to be messed with. I don’t remember if any got in my mouth but my eye definitely felt how spicy it is!

    • @RoosterMontgomery
      @RoosterMontgomery 9 หลายเดือนก่อน +2

      I thought these cacti only occur in Africa.

    • @imperialleather5448
      @imperialleather5448 8 หลายเดือนก่อน +8

      That must have been pure agony😢

    • @la24dogg21
      @la24dogg21 8 หลายเดือนก่อน +1

      WHOAOHHHH

    • @pbjandahighfive
      @pbjandahighfive 8 หลายเดือนก่อน +18

      @@RoosterMontgomery They are native to Northern Africa, yeah, but that doesn't mean they can't be grown elsewhere.

    • @elicarlson7682
      @elicarlson7682 8 หลายเดือนก่อน

      Wow

  • @HakureiMikko
    @HakureiMikko ปีที่แล้ว +217

    Roughly twenty years ago, a few mates and I discovered the effects of euphorbias. By some weird coincidence, the huge spurge in the window sprung a slight leak and "someone", who may or may not have been myself, decided to taste the latex. I wouldn't call it spicy or pungent but definitely numbing, as in having a totally desensitized spot on my tongue for two hours.
    Now, seeing as large amounts of alcohol was involved, this quickly became a component in a punishment game to everyone's delight, and severely slurred speech. No lasting effects, but in retrospect quite stupid.

    • @jasonrobbins4227
      @jasonrobbins4227 ปีที่แล้ว +13

      It is stories like these that spawned the phrase "live without regret" 🤣🤣🤣🤣🤣🤣

    • @outerrealm
      @outerrealm ปีที่แล้ว

      Ask any good chemist - alcohol = liquid stupidity. Same effect can be had by repeatedly hitting yourself in the head with a hammer.

    • @outerrealm
      @outerrealm ปีที่แล้ว +4

      @@jasonrobbins4227 translation: live stupidly, repeating all your mistakes as well as making new ones.

    • @curve5746
      @curve5746 ปีที่แล้ว +4

      Lol all men who make it to 30 are lucky

    • @timaa.4379
      @timaa.4379 ปีที่แล้ว +15

      funnily enough, resiniferatoxin is now being tested in clinical trials as a potential painkiller, so your observation had a lot of merit!

  • @dw4223
    @dw4223 ปีที่แล้ว +392

    Nile Red is literally one of my favorite channels and I'm always on the look out for more great content in the same vein. You've got a new follower in me, so keep up the good work!

    • @LabCoatz_Science
      @LabCoatz_Science  ปีที่แล้ว +48

      I appreciate that! I should have a new video coming out in a few days covering an easy OTC route to acetic anhydride, if that sounds interesting to you!

    • @megamaser
      @megamaser ปีที่แล้ว +22

      @@LabCoatz_Science Yes that will be a very interesting video for those looking to synthesize heroin I mean aspirin in their basement.

    • @stopthephilosophicalzombie9017
      @stopthephilosophicalzombie9017 ปีที่แล้ว

      @@LabCoatz_Science I think you should do a peyote extraction after that. : )

    • @chypearce8457
      @chypearce8457 ปีที่แล้ว

      Same. I literally subscribed as soon as he name-dropped

    • @RaptureDragon1
      @RaptureDragon1 ปีที่แล้ว

      Check out "I did a thing" on TH-cam if you haven't seen him before =D you might enjoy too.

  • @juggernautonfire
    @juggernautonfire ปีที่แล้ว +141

    Great video! Also a quick side note / potential further explanation as to why the isolated RTX fell short of the spiciness of the habanero: The capsaicin in chilis is dissolved in the oleoresin which acts as a carrier to distribute it to the taste receptors. NileRed Shorts featured a video of him trying pure capsaicin and the results were quite similar to your test of RTX. Dissolving the capsaicin in the solubilizer ethanol immediately unleashed its full potency. Maybe that's what was going on here as well

    • @LabCoatz_Science
      @LabCoatz_Science  ปีที่แล้ว +44

      Thanks! For the test, a small amount of ethanol was added (I think I actually mentioned that in one of the video text pop-ups).

  • @TheToemeister
    @TheToemeister ปีที่แล้ว +65

    I’d try dissolving the extract in vegetable or canola oil instead of ethanol for the taste test. I’ve read that capsaicin in oil tastes spicier than pure capsaicin due to the better solubility, allowing it to distribute across the tongue more easily. A lot of the ethanol probably evaporated off the toothpick while you were holding it too.

  • @pyrojack8230
    @pyrojack8230 ปีที่แล้ว +34

    Man was so disappointed with spicy foods he went out of his way to make Raytraced Spice. Absolute legend.

  • @ElementalMaker
    @ElementalMaker ปีที่แล้ว +93

    Holy smokes man you are brave! I remember going down a rabbit hole researching rtx, and was pretty terrified from my reading. You just smeared that toothpick around your tongue like a madman! Truly impressive isolation and testing. Glad it didn't melt your tongue away. Subbed!

    • @LabCoatz_Science
      @LabCoatz_Science  ปีที่แล้ว +22

      Thanks man, glad you liked the video! I honestly kinda wish is was a bit more intense, but I think it turned out well in the end. Next up for me, synthesizing thioacetone: the most putrid substance on Earth. We'll see if that triggers a more dramatic reaction from me, lol!

    • @ElementalMaker
      @ElementalMaker ปีที่แล้ว +2

      @@LabCoatz_Science Looking forward to it!

    • @au8363
      @au8363 ปีที่แล้ว +2

      Jesus Is King.!!!

    • @lowkeylysemith2568
      @lowkeylysemith2568 ปีที่แล้ว +8

      @@au8363 King of the fictional characters?

    • @au8363
      @au8363 ปีที่แล้ว +2

      @@lowkeylysemith2568 Jesus Is Very Real Deuteronomy 4:29 King James Bible
      But if from thence thou shalt seek the LORD thy God, thou shalt find him, if thou seek him with all thy heart and with all thy soul.

  • @stopthephilosophicalzombie9017
    @stopthephilosophicalzombie9017 ปีที่แล้ว +1

    I love videos like this that ask a simple question and then go into detail why it has a complex answer. You make great educational videos which I'm sure will inspire many a future chemist and/or scientist.

  • @antozon5070
    @antozon5070 ปีที่แล้ว +72

    Please keep in mind that the phorbol esters (to which RTX is related) are well known tumor promoters!
    Also, I strongly suspect that the vanillin stain is meant for TLC analysis. Given that your extract still contained other compounds it really is no surprise that vanillin/sulfuric acid turns black.
    But I do appreciate that you distinguished higher scoville rating and spicyness, something too many people gloss over. Also, kudos on the high production quality and cinematography, well done!

    • @LabCoatz_Science
      @LabCoatz_Science  ปีที่แล้ว +17

      Thanks, those are all excellent points! The stain test was meant for TLC, but I figured it would react the same in the vial as it would on a TLC plate, since both are just inert silica-based surfaces. I knew that the stain turning black wasn't a definite sign, but I figured I would still include it as a cool visual with at least some merit.
      Regarding the phorbol esters, I knew they would be present during the tasting, but I (questionably) rationalized doing the test anyways. In my caveman brain, the cancer risk from tasting a single drop of it was probably less than the risk from smoking a pack of cigarettes (something some people do daily). Not the best decision, but I'm hopeful that things will turn out alright in the end.

    • @antozon5070
      @antozon5070 ปีที่แล้ว +9

      @@LabCoatz_Science Oh, it'll react the same in the vial to the stain, of course. But you can't really say if it's because of RTX or because another compound or because of general tar formation. Vanillin/sulfuric acid is a fairly general stain for organics and if you get a mixture of all colors that will also be black. On a TLC plate you have the compounds already (mostly) separated from each other.
      Regarding consumption, it's going to be fine, just don't actually make a hot sauce out it lol

    • @user255
      @user255 ปีที่แล้ว +8

      @@LabCoatz_Science Yes, they are not that carcinogenic. Many plants have them and the typical response is just rash. Chronic exposure is another deal.

    • @au8363
      @au8363 ปีที่แล้ว +4

      Jesus Is King.!!!

    • @marshallbowdrie8562
      @marshallbowdrie8562 ปีที่แล้ว +12

      @@au8363no he isn’t

  • @Finnnicus
    @Finnnicus ปีที่แล้ว +3

    I’ve never seen someone do a diy column on chemistry TH-cam. Congrats! Yours was thoroughly insane using ground up kitty litter, so I’m glad it was the first I’ve seen.

  • @davidmcdonald7775
    @davidmcdonald7775 ปีที่แล้ว +2

    Great video - thanks very much for your contribution to science. I can't believe it took me this long to discover you, but that's me now subscribed. I look forward to flicking through more of your vids! :)

  • @Sleepyken900
    @Sleepyken900 ปีที่แล้ว +5

    Honestly! These videos are legendary... you deserve millions of subs!

  • @HistoricallyAccurate247
    @HistoricallyAccurate247 ปีที่แล้ว +8

    Very interesting video ! I can't wait to see your new sstc ! I built your world's easiest sstc and I run it on 6 amps .The results were impressive!I subscribed:)

  • @sealpiercing8476
    @sealpiercing8476 ปีที่แล้ว +23

    If you get your hands on the pure compound, please please please start with some dilutions or other known small quantities. Give yourself a chance to spot problems before they become big problems... Love your work.

  • @tylernethery409
    @tylernethery409 ปีที่แล้ว +1

    First video i've seen from you.. late to the party but im glad to see Nile have some competition. I love this type of science and im glad to see others providing content as quality as this! wish you the best and ill be wathing your other videos!

  • @dotech4128
    @dotech4128 ปีที่แล้ว

    You deserve more subscribers. So much improvisation and ingenuity.

  • @OmegaGrunt
    @OmegaGrunt ปีที่แล้ว +15

    That's the thing about the scoville scale that I don't think a lot of people understand, something can be noticeably hotter at a lower number, its just easier to dilute. It's not really a measure of how spicy something is but how potent it can be at smaller doses. A good example would be on hot ones, people go crazy over 'da bomb' at 100k but handle the several million last dab fairly well.

    • @TheDaringPastry1313
      @TheDaringPastry1313 ปีที่แล้ว +3

      Yeah, that's because da bomb lies since it has real pepper extract in it and is more like 1.1M on the scale O_O

    • @BixbyConsequence
      @BixbyConsequence ปีที่แล้ว

      @@TheDaringPastry1313 Yeah you can really taste the capsaicin resin in it. And nothing else, unfortunately. It has no redeeming flavors at all, which is why it seems so diabolical.

    • @travistucker7317
      @travistucker7317 5 หลายเดือนก่อน

      The show with hot questions and even hotter wings?

  • @Goazbox
    @Goazbox ปีที่แล้ว +5

    Just wanted to take the opportunity to mention your channel is way way waaaaaaay underrated with 19k subscribers. You deserve millions!

    • @LabCoatz_Science
      @LabCoatz_Science  ปีที่แล้ว +2

      Thanks man! My next video will either be synthesizing a superacid or smelling selenoacetone...hope those sound interesting!

    • @Goazbox
      @Goazbox ปีที่แล้ว +1

      @@LabCoatz_Science both do. Keep it coming! Greetings from the Netherlands!

  • @DanniDuck
    @DanniDuck ปีที่แล้ว +2

    Seeing you like all the comments, even new ones, is worthy of a sub. Good work

  • @Johnfromthedesert
    @Johnfromthedesert ปีที่แล้ว +2

    Congrats, I think this video just got bumped up by the TH-cam algorithm. Got a new sub here, you madlad. Keep up the good work... "responsibly." 😉👍

  • @MrApokalipse666
    @MrApokalipse666 ปีที่แล้ว +16

    If you want to use cat-litter-silicagel for chromatography I would recomend to dissolve it in aqueous NaOH and precipitate/ wash it with HCl for a few times bevore using it, so you will get much more surface area and a better separation.

    • @LabCoatz_Science
      @LabCoatz_Science  ปีที่แล้ว +6

      I would have, but I wasn't in the mood to waste use up all my lye and HCl. If I was to do it again, I would do it with a proper gradient elution (pure petroleum ether to pure methanol). After, of course, I washed the silica with methanol to get rid of that (albeit inert) blue dye. I tried using acetone, water, and even gasoline to get rid of it, but to no avail. Only once I started the methanol elution did I discover what it is soluble in, lol.

  • @valiantwarrior4517
    @valiantwarrior4517 ปีที่แล้ว +7

    Very interesting about the comparison to capsaicin and how the Scoville scale works. I’ve noticed that the Scoville measurements of different substances doesn’t equate directly to how they affect those who ingest them.

  • @au_gmentedreality
    @au_gmentedreality ปีที่แล้ว

    Good job dude! I enjoyed the breakdown science. And great delivery too! Viva science

  • @seanmills7255
    @seanmills7255 ปีที่แล้ว +1

    Earned a sub from me. I love science and love how much information you gave during the experiment

  • @Nefville
    @Nefville ปีที่แล้ว +17

    OMG I have this cactus. I've had it for 20 years and I grow Carolina Reapers, the new variety. Have my first Scotch Bonnets overwintering... Okay yeah this is way out of my ability to make. Still its really cool that of all the plants I've had, this one I've had the longest, since I was a kid and all this time I had the spiciest substance on Earth while at the same time I became a huge spicy pepper fan and cultivator. Great video, unexpected results. It makes sense though.

    • @intensecutn
      @intensecutn ปีที่แล้ว +2

      Fun fact: Euphorbia are not a cactus. They are a succulent.

    • @Nefville
      @Nefville ปีที่แล้ว +1

      @@intensecutn I think I am wrong, after the video I was looking at other pictures of Euphorbia and they look like they bloom which mine has never done. Also its a fairly obscure plant from Morocco and I'm not sure that's something I would have gotten by accident 27ish years ago. It looks damn close though. I'm not a chemist so I'll have to stick with my Reapers and Scotch Bonnets for now but I did find a reasonable source for resiniferatoxin online.

    • @Cineenvenordquist
      @Cineenvenordquist ปีที่แล้ว

      @@Nefville Good luck getting that cactus or other succulent to bloom and surviving a resin tap from it if you're drawn to make forbidden negrito from it.

  • @neayt9952
    @neayt9952 ปีที่แล้ว +8

    This is a really spicy video

  • @chikkenbonz
    @chikkenbonz ปีที่แล้ว +1

    Awesome video! This was very interesting. I thought pure cap was the pinnacle of pepper pain! Watch, in another 10 years pepper growers will figure out a way to grow RTX peppers...
    But yeah your channel ----> instant sub. Gotta see more man! Love the science & experiments!

  • @narkedandafraid
    @narkedandafraid ปีที่แล้ว +1

    Glad to see your channel growing! You’ve got talent.

  • @unslept_em
    @unslept_em ปีที่แล้ว +12

    scientists have a long and storied history of going "this seems interesting. maybe i will taste it" and this is certainly one of those moments lol

  • @fazergazer
    @fazergazer ปีที่แล้ว

    Immediately subscribed and look forward to seeing your work🎉

  • @establisha
    @establisha ปีที่แล้ว

    loved the longer coontent, keep it up bro!

  • @k-aw-teksleepysageuni8181
    @k-aw-teksleepysageuni8181 ปีที่แล้ว +16

    I recently went down the "what';s the spiciest thing?" rabbit hole and found out about RTX. It is an amazing chemical that could possibly help people who suffer from chronic pain like myself. The fact it takes longer to act on pain receptors could make it a good candidate to study ways to make medications that target muscle and tissue flare ups. I get terrible neck, back, and rib/sternum pain so something like that would be a new lease on living.

    • @AldoSchmedack
      @AldoSchmedack ปีที่แล้ว +2

      As someone on morphine for pain and disabled for years I would be very interested. Has to be a better med for pain.

    • @k-aw-teksleepysageuni8181
      @k-aw-teksleepysageuni8181 ปีที่แล้ว +2

      @@AldoSchmedack It's terrible. I had to quit all opiates years back and rely on cannabis derived medicine. Often oil concentrate orally and vapourized for quick relief. CBD, THC, and the terpenes in cannabis are decent for pain, but they just keep me alive and without much complaint.
      The only problem is it's not completely numbing like opiates are. It's not like I can go out and lift things for more than 30 minutes without hitting 7s to 10s on the pain scale.

    • @jetsh1ftman746
      @jetsh1ftman746 ปีที่แล้ว

      ....... Trade pain for pain🤔. Sounds self destructive

    • @k-aw-teksleepysageuni8181
      @k-aw-teksleepysageuni8181 ปีที่แล้ว +1

      @@jetsh1ftman746 You don't understand pharmaceuticals, clearly. This substance could be used to treat nerve receptors and "numb" them out of over-firing. They've started studying it recently, and it's clearly got potential.

    • @jetsh1ftman746
      @jetsh1ftman746 ปีที่แล้ว

      @@k-aw-teksleepysageuni8181 you don't understand sarcasm clearly. This substance could be used to treat Karen syndrome and make you a better person in general.

  • @boscorner
    @boscorner ปีที่แล้ว +11

    I heard about this way back on vsauce and how it was used in trials to stop pain in terminally I'll dogs . Very interesting chemical

  • @z_monty
    @z_monty ปีที่แล้ว +2

    Very informative, subbed!

  • @Ulvens
    @Ulvens ปีที่แล้ว

    First time here. Nice concept, and video. Glad to see someone doing full format chemistry again. Nile mostly does shorts nowadays, and I'm not into it.

  • @modolief
    @modolief ปีที่แล้ว +5

    Dude, you are the coolest ... I want to be a bad ass lab tech like _this_ when I grow up!

  • @theantiorthodoxclothingcom4152
    @theantiorthodoxclothingcom4152 ปีที่แล้ว +7

    Great video! I was wondering if anyone was insane enough to eat this

  • @spynae
    @spynae ปีที่แล้ว

    God I love video lab reports, you've got yourself a new subscriber

  • @blbmnm5939
    @blbmnm5939 6 หลายเดือนก่อน

    u're not normal :D, stumbled upon ur channel via the thioacetone video done by that nilered guy or smth, but u are just next level

  • @THYZOID
    @THYZOID ปีที่แล้ว +14

    Great video! I tasted the white stuff of the same cactus once but it wasn´t really spicy. Unfortunately it died so no large scale extraction on my part. You didn a great job anyways so I don´t have to extract it anymore. Hope the phenylacetylrinvanil tastes spicier because of higher solubility. I´ll add the part were you tasted this stuff to my phenylacetylrinvanil video if you are cool with it.

    • @LabCoatz_Science
      @LabCoatz_Science  ปีที่แล้ว +8

      Glad you liked the video! Good luck with your phenylacetylrinvanil project, and yes, you can definitely use parts from my video in it if you want.

    • @THYZOID
      @THYZOID ปีที่แล้ว +2

      @@LabCoatz_Science thanks man!

    • @coopergates9680
      @coopergates9680 ปีที่แล้ว +1

      @@THYZOID Is phenylacetylrinvanil also toxic, despite not having 'toxin', or could one safely make a salsa or sauce that has it?

    • @THYZOID
      @THYZOID ปีที่แล้ว +2

      @@coopergates9680 Given the lack of information on it just assume it is very toxic

  • @sillymizu
    @sillymizu ปีที่แล้ว +3

    WHY IS THIS SO UNDERRATED

  • @syncroslicer
    @syncroslicer ปีที่แล้ว

    Great video dude! Super interesting!!!

  • @AngryK1tty
    @AngryK1tty ปีที่แล้ว

    Been waiting to see some videos on this stuff

  • @ffwast
    @ffwast ปีที่แล้ว +3

    My entire digestive tract assures you the heat of a habanero does not go away quickly.

  • @djdrack4681
    @djdrack4681 ปีที่แล้ว +5

    Hmm. So you saw my comment on a previous video about trying this? Super excited to see the attempt before trying it myself.
    I find it to be one of those molecules I'm always surprised nobody talks about despite how utterly intense RTX is:

    • @LabCoatz_Science
      @LabCoatz_Science  ปีที่แล้ว +2

      I might've seen your suggestion, but I don't recall, lol. You might actually be more surprised at how NOT intense RTX is! You'll see it in the video, but RTX is possibly less "spicy" than capsaicin, despite being higher on the Scoville scale. The large molecular size stops it from penetrating nerve receptors very fast, and it's so overpowered that it tends to desensitize rather than excite like capsaicin. It's still spicy though, but in a very unique way...you'll just have to see once the video airs!
      Side-note though, I'm unsure of the purity of my sample, so I can't say that my reaction truly captures what pure resiniferatoxin is capable of. Based on my research, it should be less than 10% RTX by weight, since I failed to fully separate the other diterpene esters with chromatography. Still, there was enough to taste, which attests to it's high position on the Scoville scale!

    • @djdrack4681
      @djdrack4681 ปีที่แล้ว +2

      @@LabCoatz_Science Brutal. I was hoping for it to be used as the deterrent in 37mm smokes used to deter the various trespassing bigfoot on the property. :_(

  • @SaltNBattery
    @SaltNBattery ปีที่แล้ว +1

    Really cool video. Your channel deserves more subscribers.

  • @davida1hiwaaynet
    @davida1hiwaaynet ปีที่แล้ว +1

    Fascinating! I have always enjoyed habañero peppers with my Mexican and West Indian food. Didn't know there was a (supposedly) more potent active ingredient than capsaicin. Thanks for showing this and tasting it!

    • @totalmetaljacket789
      @totalmetaljacket789 5 หลายเดือนก่อน

      The tilde in habanero is actually a common misspelling.

  • @jkfury.x1254
    @jkfury.x1254 ปีที่แล้ว +3

    11:42 you could see the fear in his eyes

    • @LabCoatz_Science
      @LabCoatz_Science  ปีที่แล้ว +1

      Pfff, nah! That was honestly more sleep deprivation than anything, lol. Living with five other people (and pets) means I have to stay up pretty late to get shots like this done without background noise.

  • @NeoMarv
    @NeoMarv ปีที่แล้ว +5

    Nice video. But as a chemist I see many problems with that isolation. Resiniferatoxin contains many labile functional groups. You have an alpha beta unsaturated ketone, an ester and three epoxides which are connected by a single carbon and one of the epoxides is even an allylic epoxide which easily opens up when even seeing a nucleophile. Heating that molecule in water/MeOH with all the other compounds in the crude mixture most probably leads to decomposition. I am interested what the pH was in the heated mixture... If it's even slightly acidic or basic it's even worse. So you might not have tasted the actual resiniferatoxin but a derivative of it. Also when you say that the product was confirmed by a colour reagent, then that's just an assumption. Using the Rf value of a TLC on top of it would have been better but still no complete verification. I don't try to be negative but simply put I love organic chemistry and I do try to add information whenever ppssible.

    • @LabCoatz_Science
      @LabCoatz_Science  ปีที่แล้ว +3

      I totally agree with your assessment. The pH was fairly neutral, iirc, but I do think the prolonged heating probably caused a lot of unwanted decomposition. I do believe there was enough RTX to taste though, since I did try applying the raw latex to my tongue and I got the same effect (albeit somewhat weaker). That kinda leads me to another point: the plant was dying when I did the synthesis, and it didn't seem to have as much RTX as it theoretically should have. I've heard stories about these plants giving debilitating burns and discomfort just by chopping them up, and this one produced no such effect.

    • @NeoMarv
      @NeoMarv ปีที่แล้ว +1

      @@LabCoatz_Science I agree with you and the potential derivatives might also have a similar effect though. It would actually be nice to find out which compounds were in your final product but that would be quite costly and time consuming. I think this was a nice project. I did not know about Resiniferatoxin before and that's what's so good about such videos, you learn new things in an entertaining way!

  • @JasonLuther1
    @JasonLuther1 ปีที่แล้ว

    great content. glad I found you

  • @jaycobwhaley5509
    @jaycobwhaley5509 ปีที่แล้ว

    Just discovered you, and wow! Your content is fucking great! Very informative and extremely well done. Thank you!

  • @SaltNBattery
    @SaltNBattery ปีที่แล้ว +5

    I had a panic attack from trying Carolina Reaper...
    I couldn't talk myself out of it, thought I was having an anaphylactic reaction or something. Pretty embarrassing but I have Panic Disorder, so it doesn't take much.

    • @LabCoatz_Science
      @LabCoatz_Science  ปีที่แล้ว +3

      Me too, to a lesser degree, although I'm getting better with it now!

    • @jerseylife8701
      @jerseylife8701 ปีที่แล้ว +1

      I could definitely see a reaper causing a panic attack lol.

    • @EloNaj
      @EloNaj ปีที่แล้ว

      @@jerseylife8701 Yes I grew reaper but I would never try to eat them raw. Never ever XD.

  • @Sparkette
    @Sparkette ปีที่แล้ว +3

    This video sponsored by NVIDIA

    • @Ori-lp2fm
      @Ori-lp2fm 23 ชั่วโมงที่ผ่านมา

      Underaged comment

    • @Ori-lp2fm
      @Ori-lp2fm 23 ชั่วโมงที่ผ่านมา

      Underated

  • @chomp_5412
    @chomp_5412 ปีที่แล้ว +1

    Great vid!

  • @randomdudeface
    @randomdudeface 8 หลายเดือนก่อน +1

    Yo this was really interesting!
    Also I *LOVE* spicy stuff, so... 🤔 I should make a hot sauce out of this stuff sometime...😁

  • @morestupidforms
    @morestupidforms ปีที่แล้ว +3

    Water is the last thing you want with spices, it closes the taste buds and traps the spices in. You want lots of milk.

  • @Piocoto123
    @Piocoto123 ปีที่แล้ว +6

    Pretty cool. I am really into cacti and euphorbias and have tried a coiuple of their latex directly. Tried Euphorbia aeuruginosa.. mehh, tried euphorbia parciramulosa.. holy hell, the heat was there for about an hour and was as bad as a very hot sauce. I have an E. resinifera but after trying the other one I dont think I need to try that one ever

    • @LabCoatz_Science
      @LabCoatz_Science  ปีที่แล้ว

      Honestly, I tasted the latex directly, and it wasn't bad. I'm beginning to think my euphorbia was on the more mild side, since it didn't even irritate my skin or make my eyes water (although it was spicy). Not sure what is in the euphorbia parciramulosa latex though, but if it lasted for about an hour, it might've been resiniferatoxin, tinyatoxin, or a similar "super-capsaicinoid".

  • @Strothy2
    @Strothy2 ปีที่แล้ว

    You deserve way more subs ma dude!

  • @createvideo561
    @createvideo561 ปีที่แล้ว

    You are incredible and the compound you are making is tremendously spicy

  • @liamkendell806
    @liamkendell806 10 หลายเดือนก่อน

    Good informative vid!

  • @bud8254
    @bud8254 6 หลายเดือนก่อน

    Awesome video! You earned yourself a new sub my friend.

  • @actoryz2049
    @actoryz2049 ปีที่แล้ว +2

    This video is underrated

  • @allenhonaker4107
    @allenhonaker4107 ปีที่แล้ว +1

    Very Rod Sterling ish intro. I love it♥️

  • @ADHDWOOHOO
    @ADHDWOOHOO ปีที่แล้ว

    Man the pure DIY energy here is awesome.
    I've had pure capsaicin before and the sensation was kind of similar to yours. I then drank a beer and my life was instantly ruined. I wonder if you dissolved the RTX in ethanol it would hit harder.

  • @KratsminschSkunk1
    @KratsminschSkunk1 7 หลายเดือนก่อน

    I had two skunks who liked habaneros as much as I did. Agent Cooper, and Professor Moriarty. I had to record and post a video of them eating one. I wouldn't try what you did, but it's been intriguing enough I watched you through the whole process.

  • @danielengsvang3126
    @danielengsvang3126 ปีที่แล้ว

    Perfectly "Nerdy".
    Just brilliant! . I love stuff like this 🤗
    Good Job mister.
    Would have been fun to mix some of it with "Salpeter/Sugar" to make burning pepper spray smoke 💌🤫

  • @daniel-wood
    @daniel-wood ปีที่แล้ว +2

    I like how you just casually mention distilling gasoline to get the petroleum ether

  • @strangehappenings8961
    @strangehappenings8961 ปีที่แล้ว

    This guy gives some real David Hahn vibes. Haha cool video man. 📹

  • @alcyonecrucis
    @alcyonecrucis ปีที่แล้ว

    Very good work. You’re an excellent chemist and a joy to watch :)

    • @LabCoatz_Science
      @LabCoatz_Science  ปีที่แล้ว +3

      Thanks, although I wish all my videos had the quality my newest ones do, lol. Sometimes I look back at videos like this one and cringe at all the little things I could've improved, haha!

    • @OhhCrapGuy
      @OhhCrapGuy ปีที่แล้ว +1

      @@LabCoatz_Science One of the things I personally enjoy most about finding new channels is going back through their catalog and getting an idea of how they've improved over time. The improvement is actually a huge indicator of how much a creator cares about their craft.

  • @sarthakganguly5684
    @sarthakganguly5684 ปีที่แล้ว

    Cool looking setup, especially beakers

  • @Xavierthedood4123
    @Xavierthedood4123 ปีที่แล้ว +1

    Awesome video 🎉

  • @rex-up9ln
    @rex-up9ln ปีที่แล้ว

    Damn! This is exactly what I was looking for

  • @user-po7iv4ni3o
    @user-po7iv4ni3o ปีที่แล้ว +1

    12:20 - "So one of the first things you notice whenever you're tasting resiniferatoxin... " I agree, I noticed that during my last session 🤣

  • @romanmir01
    @romanmir01 5 หลายเดือนก่อน

    something has 'toxin' in its very name and you still put it into your mouth, I applaud the effort)

  • @havadd
    @havadd 6 หลายเดือนก่อน

    Your the only one on TH-cam I've been able to find with Balls enough to try this

  • @garymillwacker8486
    @garymillwacker8486 8 หลายเดือนก่อน

    LMAO!!! your face when eating hot peppers is about as dramatic as someone drumming their fingers on a desk during a rainy afternoon.

  • @dopeymark
    @dopeymark ปีที่แล้ว

    Full commitment recognized.

  • @nathanatwine3820
    @nathanatwine3820 ปีที่แล้ว

    Good job mate

  • @offthedockproductions7151
    @offthedockproductions7151 7 หลายเดือนก่อน

    Good job!!

  • @rollbot
    @rollbot 16 ชั่วโมงที่ผ่านมา

    wow! great write up video

  • @321tryagain
    @321tryagain ปีที่แล้ว

    Really interesting, thanks

  • @salamance2
    @salamance2 ปีที่แล้ว +1

    underrated channel subd.

  • @jonadams8841
    @jonadams8841 ปีที่แล้ว +1

    30 years ago, when I bought a house in the San Gabriel foothills north of LA, I was told that the incredible assortment of euphorbia cacti all around the house and property had been collected as cuttings, by the previous owners in their global adventures. There were dozens of types, from low shrubs to tall tree-ish things. And thorns and spines. Some had metal tags that gave the formal name. Again, I was told that the white sap/resin/latex that would ooze forth when the plant was damaged would burn the skin, and if inhaled, or exposed to the eye, would necessitate medical treatment.
    The Internet was not quite a thing, but the LA County Arboretum was just down the road. There, they had many of the same plants, but I could find no one who could really tell me much about the plants, except for the pain aspect. In the decade I had that house, I never experimented with the sap. Now I wish I did 😂

  • @hunterbawlz
    @hunterbawlz 3 หลายเดือนก่อน

    Nicely done Mr. White

  • @ste887
    @ste887 ปีที่แล้ว +2

    quick tip, if you need vanillin powder later on, most stores that sell DIY vape flavours offer pure vanillin powder at a couple dollars gram for pharmaceutical purity, least, they do in the uk. with the vape laws in America things might be different

  • @fscHome
    @fscHome หลายเดือนก่อน

    My respect!
    A helpful tip beside:
    Don't drink water to neutralize the capsaicin, drink simple salad oil - this helps! 😉

  • @ImpGimp
    @ImpGimp ปีที่แล้ว

    Great Video, subbed and liked =) Keep going ^.^

  • @WilliamKKidd
    @WilliamKKidd 6 หลายเดือนก่อน

    Are we just going to gloss over you nibbling at a habanero pepper?! and then being like "Yeah, it went away in 30 seconds"?!
    Bro, you are a beast... godspeed!

  • @septixskeptix1107
    @septixskeptix1107 ปีที่แล้ว +2

    I tend to not eat compounds that end in the word "toxin." I guess I just have some kind of weird hang up with that word. 🤷

  • @henrybrewer8675
    @henrybrewer8675 ปีที่แล้ว +1

    This video is such high quality! Keep it up

  • @raygun26
    @raygun26 ปีที่แล้ว

    Amazing video.

  • @besteterzioglu9258
    @besteterzioglu9258 11 หลายเดือนก่อน

    Do you think we can desentize pain receptors on tongue with frequent use of RTX or capsaicin? I known capsaicin rinse 3 x or more a day is a treatment method for Burning Mouth Syndrome so I wonder if RTX would be more beneficial or quicker?

    • @LabCoatz_Science
      @LabCoatz_Science  11 หลายเดือนก่อน +1

      Yes, it could potentially be! I actually did a test with RTX in which I applied some to my tongue and then sampled a hot pepper. The area affected by the RTX felt almost no distinguishable heat, even though the rest of my mouth felt the spiciness! RTX could also be superior to capsaicin in this area because it is a slower-acting TRPV1-agonist that is geared more towards desensitization than pain-triggering (hence why it is actually being considered as a specialized pain relief option).

  • @TomMeehanMake
    @TomMeehanMake ปีที่แล้ว +1

    Great video! My only question is why you were heating a round bottom flask on a hot plate instead of placing the round bottom flask into an oil or water bath on the hot plate?

    • @LabCoatz_Science
      @LabCoatz_Science  ปีที่แล้ว

      Glad you liked it! Honestly, I was just in a rush and didn't have the proper glassware for a water/oil bath at the time. In hindsight, I could've probably used my largest beaker as a dish for such an immersion bath, but I don't think it was on hand during filming...

  • @anthonyrickardii6198
    @anthonyrickardii6198 ปีที่แล้ว +2

    Definitely indeed interesting and awesome video. I wonder what would happen if you used it as a mace?

    • @LabCoatz_Science
      @LabCoatz_Science  ปีที่แล้ว +5

      Honestly, it might be about the same as capsaicin. According to the reports I read, the powdered latex from the resin spurge was apparently used in "ye olde days" by pranksters to make people sneeze and cough, so its use as a Mace-type irritant isn't out of the question!

    • @anthonyrickardii6198
      @anthonyrickardii6198 ปีที่แล้ว

      @@LabCoatz_Science that’s indeed interesting. Thank you

  • @amp4105
    @amp4105 ปีที่แล้ว

    awesome, im wondering could this be weaponized (hypothetically) and sprayed like mace? if so surely it would cause blindness?

    • @LabCoatz_Science
      @LabCoatz_Science  ปีที่แล้ว

      Maybe, but I doubt it would be worth the effort. Pranksters in the medieval times apparently used the powdered latex for the purpose of getting people to sneeze and cough, so it is "weaponizable" to some extent. But getting pure RTX takes a lot of time, materials, and money. So for the time being, things like CS gas are still the best option.

    • @amp4105
      @amp4105 ปีที่แล้ว

      @@LabCoatz_Science fascinating, thanks!

  • @tfvfdeee7647
    @tfvfdeee7647 ปีที่แล้ว +1

    ur channel is underrated

  • @a753951852
    @a753951852 ปีที่แล้ว +1

    That's it.. Subscribed..

  • @DaffroDuck
    @DaffroDuck ปีที่แล้ว +1

    My mouth started watering when you ate that habanero

  • @Howtoeatrocks
    @Howtoeatrocks ปีที่แล้ว

    I just had a flashback of cutting up cacti in my grandmothers back yard for a “potion” and the resin getting in my eye. I had hoped to never remember