Thank you so much for the very helpful information that you provide 😊 very happy with your videos i wish we had found your you tube videos months ago . Look forward to more of your you tube videos about breeding and puppies .
Hi James! Thank you so much for all your wonderful informative videos. In this video, you say with this machine, it helps with the timing of c-sections. What level does she have to be in order for it to be safe to open her?
Are there any Canine Progesterone tests available via Saliva swabbing please? I breed Papillons & hate subjecting them to multiple jabs when tracking LH surging etc.
Nicely done sir. And what a beautiful Frenchie she is. As a Paramedic, you gave me the willies waving that sharp around😁 You clearly have lots of experience, thank you for passing it on. Subbed, as our bitch was AI'd about 18 days ago. Due a scan at 30-35 days.
How long are you supposed to let it clot? How fast should the centrifuge spin? How long typically do you need to spin it? We keep getting blood in it and it won’t separate correctly, serum stays pink not clear.
Hi I have the same small centrifuge how long would I spin a blood sample for? As the one I have goes from 0 to 7000 rpm I’m also using the finecare progesterone machine.
How long did you spin the blood down for? Any difference in running plasma vs serum? Is there a way to check accuracy of the analyzer if you believe it maybe be reading too high/low?
The Dlab centrifuge is not a good one. Mine went out on me for no reason during a test. I lost customers because of it. 😢 four e is a better brand I am learning. Also, you might want to add that there should be NO BUBBLES in the serum/ buffer mix when you put it into the window of the cartridge.
I have ichroma d I see people saying I need to incubate my test strips before running test but I can’t find a ichamber or fincubator? Do you sell this or can I just leave rest out for 15 mins then rest?
I just seen this and noticed you switched from the target test. And I was thinking about ordering the target test. Do you no longer believe they work? Curious on why you switched to this method. Thanks
In my opinion they both work. Target is more of a color test. If you go to vet you get numbers like this machine. Both will work but this machine is above the target testing.
I have female German shepherd . She was in heat. On 9th day her progesterone level is 16 ng /ml . Upto day 14 she allowed for mating. But she bleeds upto 21 days. On 21st day her progesterone level again drops to 1.6 ng/ml. Is there any problem with her ?
So why is it that every time my dog goes to have her progesterone levels tested they make me get an x-ray so I end up spending $300 per visit for a “progesterone” check?
I’m having the same problem I got some work on my female today and it dropped to 8 is that normal? Am I too late or should I try again in a couple of days
Is it normal for the level to drop? My girl started her heat on last Sunday and by Wednesday she was at 1.87 and today(Saturday) she is at 1.47. The place we took her says it’s normal and to bring her back in 3 days
You forgot to mention that the centrifuge needs to be loaded evenly in weight otherwise they vibrate very badly shortening the lifespan of the machine and potentially harming tests
I'm using it and found that the test results weren't up to the standards. I compared the results of the two tests. The results of the two matches But the results from this machine are not correct. and is highly anticipated
P.S. a smaller syringe actually makes MORE of a vacuum/negative pressure against the lumen/wall of the vein. Larger syringes generate LESS negative pressure, thus not collapsing the vein. Also, no need for a tourniquet, just have the holder compress the medial vein (towards the dog), with their thumb. I stopped watching after these two egregious errors in an “instructional video.” …smh🙄
How is that possible. A lArge syringe moves a lot more volume for a small movement of the syringe hence more vacuum 1ml versus 13 ml 13 times as much vacuum. And for those not sure. Compare a 13ml versus 1ml to draw blood Abd see which you prefer and report back here And there are many ways to clamp the vein All work fine
@@LoveMyPups I’m not being paid to educate you on the physics of syringe vacuum. I would suggest you speak with your sales rep, and they can put you in contact with people who can explain to you the particulars of syringe physics.
YAY!! You made the video I asked for. Thank You so very much James. This is going to help us in a few days drawing blood. You’re Amazing
We live to make you happy 😃
Can you please show us an example test of how to time a C-Section with this machine
Your dog is adorable! I'm getting my vet to do this but it was good to see the process.
Need a video on levels for wondfo. Need some education on this machine. Please help.
Thank you so much for the very helpful information that you provide 😊 very happy with your videos i wish we had found your you tube videos months ago . Look forward to more of your you tube videos about breeding and puppies .
Can you do a video explaining why my serum is becoming “jelly like”?! Thanks!!
Hi James! Thank you so much for all your wonderful informative videos. In this video, you say with this machine, it helps with the timing of c-sections. What level does she have to be in order for it to be safe to open her?
Awe poor baby, she’s a good girl
Hello my frenchie is in heat what are some good food or diet to feed her in heat and pregnancy?
Aww shes adorable and she doesn't like needles 😅 , her facw expression.
Hi James, where could I get the buffer as I have tried a machine but never get a reading at all ? Much appreciate 🙏
Is this the same machine for reverse PG test?
Hi James. I sell the same product in Canada and I just wanted to say I agree with you. I enjoy my machine. Easy to use.
I really appreciate your videos very informative! Thanks
Are there any Canine Progesterone tests available via Saliva swabbing please? I breed Papillons & hate subjecting them to multiple jabs when tracking LH surging etc.
Hi James, does any supplies come with this progesterone machine? Is does not say what comes with it.
Nicely done sir. And what a beautiful Frenchie she is.
As a Paramedic, you gave me the willies waving that sharp around😁
You clearly have lots of experience, thank you for passing it on.
Subbed, as our bitch was AI'd about 18 days ago. Due a scan at 30-35 days.
Sir, I have that same microcentrifuge, how long do you spin the sample for in 10kcentrifuge? Thank you
Love from India thank you so much
She’s beautiful 😻
How long are you supposed to let it clot? How fast should the centrifuge spin? How long typically do you need to spin it? We keep getting blood in it and it won’t separate correctly, serum stays pink not clear.
How can I interpret the results to know the right day to mate the dog
Breed on a progesterone level of 15
We appreciate you 🙏🏾
Thank you boss
Can you do an AI at a level of 21-22? Ng/ml
can you supply to Australia ?
Can you run whole blood and what’s the process on that?
question for you, do I have to wait 20 minutes after taking blood before putting it in the centrifuge
I wait 5
How long is blood good after being pulled out?
Are you a vet sir? Very knowledgeable 😊 great videos
How long should the blood sit and clot before spin down
Hi I have the same small centrifuge how long would I spin a blood sample for? As the one I have goes from 0 to 7000 rpm I’m also using the finecare progesterone machine.
hi I wanted to ask since you are a great breeder I am Italian breeder progesterone 14 only inseminated once there is a possibility of how many puppies
How long did you spin the blood down for? Any difference in running plasma vs serum? Is there a way to check accuracy of the analyzer if you believe it maybe be reading too high/low?
Serum or plasma is fine
Validation is based on the numbers you see and how they progress. If in doubt compare with a lab result
You can collapse the vein your not suppose to do blood draws from the cephalic vein
Interesting I have probably done a thousand with out a single issue
The Dlab centrifuge is not a good one. Mine went out on me for no reason during a test. I lost customers because of it. 😢 four e is a better brand I am learning. Also, you might want to add that there should be NO BUBBLES in the serum/ buffer mix when you put it into the window of the cartridge.
I have ichroma d I see people saying I need to incubate my test strips before running test but I can’t find a ichamber or fincubator? Do you sell this or can I just leave rest out for 15 mins then rest?
Beautiful baby 💜
Hello what is a good buffer solution and how much to use?
Buffer comes in the containers already
Hi James. How long you spin it ?
What’s better doing the test with plasma or with blood ???
When u went thru her progesterone tests u said u were breeding her at 12? I thought u said it’s best at 15? I’m confused
U can start at 12 then hit her again the next day at 15 , as far as insinination goes
2 days after start of season...
20days after season...
Dont believe people that say they can not say best time to breed...
I was under the assumption you were looking for a level of 5 then free 2 Days later
Honestly 10-15 u can hit should best ovulation stage
Can anyone give me advice on reverse progesterone?
love your channel!!!!!
When do you do the progesterone test?
Around 10 days after the dog starts her heat
@@juniorserrano90I was told 7 days after
Curious. Why do you wait two days between breeding? Thanks in advance?
Gives the stud dog a recovery time and extend the window of opportunity on getting a dog bread properly
It depends on the results and what number she is at really
I just seen this and noticed you switched from the target test. And I was thinking about ordering the target test. Do you no longer believe they work? Curious on why you switched to this method. Thanks
In my opinion they both work. Target is more of a color test. If you go to vet you get numbers like this machine. Both will work but this machine is above the target testing.
I have female German shepherd . She was in heat. On 9th day her progesterone level is 16 ng /ml . Upto day 14 she allowed for mating. But she bleeds upto 21 days. On 21st day her progesterone level again drops to 1.6 ng/ml. Is there any problem with her ?
What machine
@@LoveMyPupsportable canine progesterone analyser
So why is it that every time my dog goes to have her progesterone levels tested they make me get an x-ray so I end up spending $300 per visit for a “progesterone” check?
Because the medical system is for profit and they've learned to do the slimy things that a typical Hospital would do
I’m having the same problem I got some work on my female today and it dropped to 8 is that normal? Am I too late or should I try again in a couple of days
Mine did the same but 3 days later she spiked up
So what is 12.72? 12.72 what? Days? mL? ng? Should have taken a close up of the print out it might have helped some.
Im interested. Do you ship to europe? I need all the Ai, testing and whelping material 😅
Yes we sell 240 v versions and ship all our products overseas
Was 8.93 to early to AI my dog
good day doc i have ah siberian husky i got her progresterone result the result is 1.5ng/ml, what day she got her 1st stud.?
Will expect Breed in five days but I get another progesterone test in two or three
Is it normal for the level to drop? My girl started her heat on last Sunday and by Wednesday she was at 1.87 and today(Saturday) she is at 1.47. The place we took her says it’s normal and to bring her back in 3 days
Yes especially in young dogs I woukd wait 4 days
@@LoveMyPups thank you! She will be 2 soon
I mate my dog today...now i took progesterone test...and its 28...is there any chance for hetting pregnant??
Yes
@@LoveMyPups thankyou...
You forgot to mention that the centrifuge needs to be loaded evenly in weight otherwise they vibrate very badly shortening the lifespan of the machine and potentially harming tests
What is the name of the smaller/quieter centrifuge?
I'm using it and found that the test results weren't up to the standards. I compared the results of the two tests. The results of the two matches But the results from this machine are not correct. and is highly anticipated
Wow easy as that
Me watching this thinking i can learn to do this part of the process at home as well... lmaoo wrong gotta drop that bag ig
Why don’t you guys breed English bulldogs
Prof
👍
When in doubt just drowned the area with rubbing alcohol 🤦♂️
The site must be cleaned before drawing the blood
Or you can just take a jugular draw😂🤦🏼♂️
👍
P.S. a smaller syringe actually makes MORE of a vacuum/negative pressure against the lumen/wall of the vein. Larger syringes generate LESS negative pressure, thus not collapsing the vein.
Also, no need for a tourniquet, just have the holder compress the medial vein (towards the dog), with their thumb.
I stopped watching after these two egregious errors in an “instructional video.”
…smh🙄
How is that possible. A lArge syringe moves a lot more volume for a small movement of the syringe hence more vacuum
1ml versus 13 ml 13 times as much vacuum. And for those not sure. Compare a 13ml versus 1ml to draw blood Abd see which you prefer and report back here
And there are many ways to clamp the vein
All work fine
@@LoveMyPups I’m not being paid to educate you on the physics of syringe vacuum. I would suggest you speak with your sales rep, and they can put you in contact with people who can explain to you the particulars of syringe physics.