Inside a CRISPR Lab
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- เผยแพร่เมื่อ 29 มิ.ย. 2024
- At UC Berkeley, CRISPR researchers are developing better gene-editing enzymes and more efficient delivery into tissues. These innovations will be used to improve the effectiveness and lower the cost of life-saving therapy for genetic disease. Take a peek behind-the-scenes to see what goes into a human genome editing experiment.
Produced by Marica Petrey and Leah Worthington
Cinematography by Mogli Maureal
Edited by Marica Petrey
Sound and color by Mogli Maureal
Featuring Lorena de Oñate, Ph.D. and Rima Mendonsa, Ph.D.
Special thanks to Megan Hochstrasser, Ph.D., Innovative Genomics Institute, UC Berkeley
Music courtesy of Audioblocks.com - บันเทิง
I've been fascinated by CRISPR Cas-9 since it's inception. I follow all the news I can get, and have read Code Breaker (which was more interesting and informative than I expected!) I'm excited to see it already restoring sight and correcting sickle cell. Thanks for this look into a lab.
very well filmed and documented. great job.
So whomever did the recording and editing did a great job
Thank you for the video! On a side note, I think this process would be an great candidate for automation. It seems that the human has to complete soooo many steps...
It is automated, they're "teaching" people to do it for $100,000 a year, which those people somehow think benefits them, but really they're just doing what a robot already does.
Excellent to see...thanks for sharing madam
Thank you for the video ❤️
Learned a lot... Liked your way of presentation
Can you please give me the details about methods of extraction that you use to get crispr cas 9
Great!
Cool job ever
Fascinating
Great video!!! She's so sweeet :3
cool lab
I get that this can be used for life-saving gene modification, but it can be much more than that.
Useful and informative video
Please can you give me the methods of extraction that you use to get crispr cas 9. Thanks
Does this lab sell the purified Cas9 protein? Can it remain active through several days of shipping?
I have never seen such lab
It doesnt actually explain the entire process. A lot of the stuff theyre doing looks like it can be done in anyones garage. Very simple process its just that no one has a video actually showing the real lab work from start to finish. If someone made a 20 minute video actually detailing everything from start to finish then anyone could be a genetic engineer if they purchased the right tools.🤓
anyone can. purchase the books, learn online, buy the tools, do the experiement, coalese findings in scientific paper, and if it gets published Id say noone would argue you are not a scientist. Prestige, social hierachy, elitsm etc. are why you would probably run into more problems than someone employed at an institution doing exactly what you do in your garage.
@@volution1160tbh I’m more interested in knowing how they’re so certain they won’t introduce their target sequence backwards to the cas9. Do they always have to find the RNA from elsewhere or are we flying actual base pairs together at scale with some sort of machine? the sheer number of molecules in every drop, and the random motion of those molecules within the swirling medium feels like way too much uncertainty.
Newsflash: You can do crispr in your garage. Nobody's even checking if you know what you're doing or not. (unless you're in europe, then it's super illegal)
@@kazekagekid It doesn't do it backwards because the guide RNA only binds to the sequence in one direction. You design it to match the target sequence and no others, but sometimes it will make a mistake if you designed it poorly.
@@kazekagekid And yes you have to give it a target sequence (already in the right order) to stitch in, or else it will just stick whatever is around in there, which will not be what you wanted.
How is the guide RNA made? does it have any special feature or is it just extracted mRNA?
What city is the Lab in?
Can i order CRISPR and deliver to out of your country?
저도 여기서 연구하고 싶어요
Please Scientist 🙏 Try to reverse the biological aging process in humans.. And also to eliminate age related diseases..
Why? I see no good coming out of it.
@@AsuraVeriwhy not
I thought I saw a comment saying this but couldn’t find it. Could you PLEASE figure out the code sequence for the vaccine, then reverse it and fix us??? I’m in California. I’ll be knocking on your door soon. I was 49 when I took it. I’m 51 now. I had heart pain, dizziness, almost passing out and no more periods. Please fix us.
The sequence for the mRNA vaccine is in the public domain. No need to decrypt it 👍🏼.
🇮🇳👍🙏🙏👍🇮🇳🧬🧬🧬🧬🌐🌐🇮🇳
They can make zombie I guess
Don't judge me.... i don't know what she is doing but that "tube scraping" at 3:32 doesn't look professional made at all 🤣
She is trying to break up the pellet at the bottom of the tube. These cells want to stick together, therefore the small vibrations help break up the clusters of cells. Flick mixing such a viscous solution of cells will not properly mix, but pipette mixing (and vortexing) could damage the cells. Eukaryotic cells are much larger than bacteria and must be treated with care, using the grate to vibrate the cells is actually pretty genius if you ask me.
@@MrLovemydog12 Yeah i know but... I mean... still don't look professional 😂 they should make a machine or something made on purpose to do that if it's something needed...
@@JohnDoe-ml1ui lol you mean you need a machine that just flicks a tube couple of times? What a waste of time and energy...
@@JohnDoe-ml1ui what machine for that simple work? wasted
You know Frankenstein was a monster right ?
huh?
That's fiction