@@Mohammad-xe2qw Nucleation is the formation of a new filament while polymerization is the elongation of an existing filament. Profilin ensures monomers are not wasted on nucleation, but rather are directed towards making existing filaments longer.
Hi, thanks for your comment. Actin filaments are polarized. Monomers bind to the filament at the '-ve' end and hence filaments grow in one direction - that is from their 'barbed end'. This first requires the 'capping proteins' to be displaced from the end of the filament. If elongation factors like formin are involved, they also 'add' monomers to the same end. You can read more on actin polarity, capping proteins & the critical concentration of G-actin in the links listed in the description
I agree that it promotes polymerization. I learned that it only binds to Actin ADP, catalyses the exchange on ADP to ATP, which catalyzes polymerization.
quick question, when F-actin is in the steady-state and is treadmilling, are actin monomers still getting added to the -ve end? I know that there is net growth at the +ve end and net loss at the -ve end, but are there still some subunits being added at the -ve end?
old comment so not really a response to you, but responding to the question: polymerization occurs on both ends just as depolymerization also occurs on both ends. however, the important thing to remember is the rate at which it all occurs, if polymerization is happening faster than depolymerization, that end is going to grow; vice versa is depolymerization is happening faster than polymerization, the end is going to shrink. the barbed (+) end is growing and the pointed end (-) is shrinking, even though both polymerization and depolymerization is taking place on both ends (but again, just at different rates) specifically for the question, polymerization can go in both directions, but growth is only taking place in one direction
People. Profilin inhibits nucleation and accelerates polymerization. These two are distinct
What is the difference,Plz?
@@Mohammad-xe2qw Nucleation is the formation of a new filament while polymerization is the elongation of an existing filament. Profilin ensures monomers are not wasted on nucleation, but rather are directed towards making existing filaments longer.
khoya nta ostora
amazing video, explanation is so clear! I finally got it :)
no audio?...i swear i checked my speakers..
Hi, thanks for your comment.
Actin filaments are polarized. Monomers bind to the filament at the '-ve' end and hence filaments grow in one direction - that is from their 'barbed end'. This first requires the 'capping proteins' to be displaced from the end of the filament. If elongation factors like formin are involved, they also 'add' monomers to the same end.
You can read more on actin polarity, capping proteins & the critical concentration of G-actin in the links listed in the description
Very nice video :) Made me understand the subject!
Profilin proteins promote polymerization and growth, not inhibition. This is wrong.
Is it possible that they promote polymerization of a stable actin filament, but prevent nucleation?
I agree that it promotes polymerization. I learned that it only binds to Actin ADP, catalyses the exchange on ADP to ATP, which catalyzes polymerization.
i was confused about this after watching this video, Thank you very much
Profilin alone inhibits the polimerisation of actin. But in the presence of formins for example, the polimerisation rate will be higher.
pls do more this is savage
quick question, when F-actin is in the steady-state and is treadmilling, are actin monomers still getting added to the -ve end? I know that there is net growth at the +ve end and net loss at the -ve end, but are there still some subunits being added at the -ve end?
I think yes.
yes
preferential addition of actin monomers to (+) end, not (-) end
Yes fr
Well show. Good information.
Nice video, but it didn't show much about critical concentration... and can polymerisation only go in one direction and not the other way?
old comment so not really a response to you, but responding to the question: polymerization occurs on both ends just as depolymerization also occurs on both ends. however, the important thing to remember is the rate at which it all occurs, if polymerization is happening faster than depolymerization, that end is going to grow; vice versa is depolymerization is happening faster than polymerization, the end is going to shrink. the barbed (+) end is growing and the pointed end (-) is shrinking, even though both polymerization and depolymerization is taking place on both ends (but again, just at different rates)
specifically for the question, polymerization can go in both directions, but growth is only taking place in one direction
Is Bowfinger a good movie?
Amazing video
3tini nmra
Amazing😍
شآم مرت من هنا pre7🐞🍀✨
Tara they said nucleation😂
There's missing information ...
So cool
Cheers!! I got it
Sick vid wanna collab?
some fuckin sound would be nice
not really
I find it so depressing xD
This process keeps you alive.
What's depressing about that?
@@jannikheidemann3805 that's the point....
merci beaucoup
you should believe in god
What? This looks trippy but has nothing to do with real protein physics.
I think that that might just be someone's animation 'reel.'
WRONG !!!!!!!! profilin promotes actin polymerisation and cofilin inhibits actin polymerisation