5:37 Don't forget to leave you hair uncovered, to get some of your technician's hair samples in the tubes for better buffering results and to confuse the hell of the Doctor when results shows that child's mother is not his mother but the mother of the technician!!!
I could be very wrong, but for a 50 microliter mix of 7 reactions you should have 350 microliters, not 340 microliters. Water should be added until 343 microliters, not 333 microliters.
you're right. I also realised that suddenly my maths did not match... was like why... and then I noticed they suddently did what you mentioned. which is a mistake since in the beginning they indeed mentioned the final volumen (for 7 reactions) was 350 ul (therefore 175 ul from the master mix aso...).good to see I was not the only one.
The video is so helpful, thank you
5:37 Don't forget to leave you hair uncovered, to get some of your technician's hair samples in the tubes for better buffering results and to confuse the hell of the Doctor when results shows that child's mother is not his mother but the mother of the technician!!!
Very important... Thanks
Do you have the reference of the ideal room?
buen vídeo
saludos
nice job!!!!!
Thnks
Video on Calculations that go into assembling such PCR reaction
th-cam.com/video/gNrNfjHO4cA/w-d-xo.html
I could be very wrong, but for a 50 microliter mix of 7 reactions you should have 350 microliters, not 340 microliters. Water should be added until 343 microliters, not 333 microliters.
you're right. I also realised that suddenly my maths did not match... was like why... and then I noticed they suddently did what you mentioned. which is a mistake since in the beginning they indeed mentioned the final volumen (for 7 reactions) was 350 ul (therefore 175 ul from the master mix aso...).good to see I was not the only one.
this video is so helpful thank you
Hi! I just want to ask, can I use your video in the project that I am making? Thanks!
Thank you
Thanks
Thank you for the video