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To calculate enzyme concentration [E] in Kcat calculation. Kcat = Vmax/[E] Example: Vmax = 200 umol/mg/min Enzyme concentration = the amount of the enzyme used in the assay or reaction from Vmax - its 1 mg. so what is the concentration of enzyme [E] in 1 mg (amount of enzyme/mg) lets say its molecular weight is 28KDa = 28000 Da = 28000 gms/mole = 28000 mg/mmol = 28mg/umol (1 mole = 1000 mmol, 1 mmol = 1000 umol) 1 mg of enzyme = 1/28 umol = 0.0357 umol so concentration of enzyme [E] = 0.0357 umol/mg Kcat = Vmax/[E] =200 umol.mg-1.min-1/0.0357 umol.mg-1 Kcat = 5602 min -1
Sorry about video quality. Can you please let us know which think is lacking in video? We really want to improve the quality and content of our video tutorials? Many thanks you watched our video and highlighted the issue
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To calculate enzyme concentration [E] in Kcat calculation. Kcat = Vmax/[E] Example: Vmax = 200 umol/mg/min Enzyme concentration = the amount of the enzyme used in the assay or reaction from Vmax - its 1 mg. so what is the concentration of enzyme [E] in 1 mg (amount of enzyme/mg) lets say its molecular weight is 28KDa = 28000 Da = 28000 gms/mole = 28000 mg/mmol = 28mg/umol (1 mole = 1000 mmol, 1 mmol = 1000 umol) 1 mg of enzyme = 1/28 umol = 0.0357 umol so concentration of enzyme [E] = 0.0357 umol/mg Kcat = Vmax/[E] =200 umol.mg-1.min-1/0.0357 umol.mg-1 Kcat = 5602 min -1
To calculate enzyme concentration [E] in Kcat calculation. Kcat = Vmax/[E] Example: Vmax = 200 umol/mg/min Enzyme concentration = the amount of the enzyme used in the assay or reaction from Vmax - its 1 mg. so what is the concentration of enzyme [E] in 1 mg (amount of enzyme/mg) lets say its molecular weight is 28KDa = 28000 Da = 28000 gms/mole = 28000 mg/mmol = 28mg/umol (1 mole = 1000 mmol, 1 mmol = 1000 umol) 1 mg of enzyme = 1/28 umol = 0.0357 umol so concentration of enzyme [E] = 0.0357 umol/mg Kcat = Vmax/[E] =200 umol.mg-1.min-1/0.0357 umol.mg-1 Kcat = 5602 min -1
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@@bioinfoxpert I have my data for multiple substrate reaction. I am not able to analyze my data. I need help to understand few basic concepts. Can you share your email address so that i can share the data?
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Hi, Hope you have enjoyed our Bioinformatics videos. Now DataScience & Bioinformatics Team is inviting you to attend an Excellent Course to Learn Complete Bioinformatics from Scratch on Discounted Prince. After taking this course, you will be very confident and we ASSURE YOU THAT THIS GAME CHANGER FOR YOU.
There are details about the Theory and Practical of
Homology Modeling
Threading, 3D Structure Prediction
Evolutionary Bioinformatics
Gene Predictions
Database Searching
Alignment
Docking
and everything you can expect. So what are you waiting for? Please click below to get the course at a discounted price.
www.udemy.com/course/learn-bioinformatics/?referralCode=35C6DA89E7B61CA56DCC
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To calculate enzyme concentration [E] in Kcat calculation.
Kcat = Vmax/[E]
Example:
Vmax = 200 umol/mg/min
Enzyme concentration = the amount of the enzyme used in the assay or reaction
from Vmax - its 1 mg.
so what is the concentration of enzyme [E] in 1 mg (amount of enzyme/mg)
lets say its molecular weight is 28KDa = 28000 Da = 28000 gms/mole = 28000 mg/mmol = 28mg/umol
(1 mole = 1000 mmol,
1 mmol = 1000 umol)
1 mg of enzyme = 1/28 umol = 0.0357 umol
so concentration of enzyme [E] = 0.0357 umol/mg
Kcat = Vmax/[E]
=200 umol.mg-1.min-1/0.0357 umol.mg-1
Kcat = 5602 min -1
Dear Sreeivas, Many thanks for your reply.
@@bioinfoxpert Welcome and I thank you for your useful video to calculate kinetic parameters.
Thank you so much sir 🙏
Comprehensively explained.
Thanks
Nicely elaborated ...Thanks Sir!!
Most welcome!
It's very useful, just the video quality got a little problem, but congratulations and thank you
Sorry about video quality. Can you please let us know which think is lacking in video? We really want to improve the quality and content of our video tutorials? Many thanks you watched our video and highlighted the issue
If you want to learn Bioinformatics from scratch then take our course on Udemy
Course Link: bit.ly/2SFH0fz
The course is with 30 days Money Back Guarantee
How did you get the total enzyme concentration
To calculate enzyme concentration [E] in Kcat calculation.
Kcat = Vmax/[E]
Example:
Vmax = 200 umol/mg/min
Enzyme concentration = the amount of the enzyme used in the assay or reaction
from Vmax - its 1 mg.
so what is the concentration of enzyme [E] in 1 mg (amount of enzyme/mg)
lets say its molecular weight is 28KDa = 28000 Da = 28000 gms/mole = 28000 mg/mmol = 28mg/umol
(1 mole = 1000 mmol,
1 mmol = 1000 umol)
1 mg of enzyme = 1/28 umol = 0.0357 umol
so concentration of enzyme [E] = 0.0357 umol/mg
Kcat = Vmax/[E]
=200 umol.mg-1.min-1/0.0357 umol.mg-1
Kcat = 5602 min -1
omg, I know this trick after graduated, we don't need to lineweaver burk equition to determine the Km and Vmax value right?
Yes you are right
Sir,how to calculate the enzyme concentration. My enzyme is 1000U/mg.can you help me.
To calculate enzyme concentration [E] in Kcat calculation.
Kcat = Vmax/[E]
Example:
Vmax = 200 umol/mg/min
Enzyme concentration = the amount of the enzyme used in the assay or reaction
from Vmax - its 1 mg.
so what is the concentration of enzyme [E] in 1 mg (amount of enzyme/mg)
lets say its molecular weight is 28KDa = 28000 Da = 28000 gms/mole = 28000 mg/mmol = 28mg/umol
(1 mole = 1000 mmol,
1 mmol = 1000 umol)
1 mg of enzyme = 1/28 umol = 0.0357 umol
so concentration of enzyme [E] = 0.0357 umol/mg
Kcat = Vmax/[E]
=200 umol.mg-1.min-1/0.0357 umol.mg-1
Kcat = 5602 min -1
sir, should we suppose the value of Et like yu did or we have to find it out?
Its 0.5 M for each
@@bioinfoxpert so the value is fixed to 0.5M or we have to find out depending on the question?
Learn a alot !Thank U so much Sir
So nice of you
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Well explained 👌
Glad you liked it
Very informative.
Glad you think so!
Lo amo caballero
We love you too. Many thanks for comment
I need some help with my data..can you help me??
How can i help you please let me know
@@bioinfoxpert I have my data for multiple substrate reaction. I am not able to analyze my data. I need help to understand few basic concepts. Can you share your email address so that i can share the data?
Datascienceandbioinfo@gmail.com
Please feel free to contact me
What is effective molarity? How do we calculate kuncat for a reaction?
thank you so much
You're welcome!
lov u
increible
Love you too
Thanks
It's Menten. Not Mental! 😂
Thanks for corrections
If you want to learn Bioinformatics from scratch then take our course on Udemy
Course Link: bit.ly/2SFH0fz
The course is with 30 days Money Back Guarantee