🔬 018 - How can one take pictures with a microscope? All about Microscope CAMERAS and ADAPTERS
ฝัง
- เผยแพร่เมื่อ 19 ม.ค. 2018
- There are several ways of connecting a camera to a microscope and in this video I give an overview of the different adapters and camera systems.
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Channel with much microscope information: th-cam.com/channels/-l-sRZPOd55p-SqleSKBNA.html#
Microscope buying advice: th-cam.com/play/PL4WoSCazHjWjmSnIfb58mTqnH_TP1sYIM.html
Good lawd man, thanks for all your insights into this. It is invaluable information for someone who needs a clue
the trick you showed at the end really works. Rarely could I get a decent photo when using my android but stabilizing it with your hand encircling the ocular's stabilizes it great. you are the man!
You videos help so much. I just purchased a T490B and am trying to create videos and pictures to compare and identify microorganisms but it’s proven difficult until now. Thank you my friend. I’ll be looking into obtaining that small digital camera setup you have there. It seems simple enough
This video helped me understand a lot of things. Thank you very much.
Thanks for the upload buddy, you did a really good job explaining things and I found it very helpful indeed. Keep up the great work
Nice job explaining the camera adapters.
Thank you for this!
Great review of techniques
Very helpful and useful
This is so cool. Nice, straight forward and very useful information on how to get quality images from a miscroscope. Thanks.
thank you for great video, cheers :)
Good job man
Super helpful! This is the exact video I was looking for to learn more about microscope photography. Thank you so much!
me too , thank you very much
Voy empezando este hoby y me es muy entretenido aprender de tu conocimiento, gracias
thank you so much for this! hard to find info on this subject
Thanks man, you just saved me so much headache!
Would like to see more about the camera bracket set up you mentioned planning on doing a video dedicated to it. Thanks
I have some computer USB ports that are 2.0 and some that are 3.0. I'm liking the idea of using my mobile phone more and more. Storage and many controls.
What a great explanation! What about the camera bracket you mentioned ? Would realy like to see a video about it.
Thanks.
it would be great if you could make a picture of the same element (or 2, at low and high magnification) with each of the setups (from the phone to the Reflex camera). If you would do an update of this video like that i´d love to watch it!
...also can you link in the description below your video, the link of the video on the setup to attach a compact camera?
.... so that would be triple thanks i suppose... hehe..
Thank you for sharing these tutorials! I recently started an Earth Science teaching job in the US and am going to give focus stacking a try on some of my better looking mineral grains. Do you have any advice for working with older microscope cameras? We have several microscope cameras (Sony, Panasonic) in my department, but they all have the old BNC video output cables. Is there hope that I could connect those to a modern laptop for digital image capture?
Great information... Can you possibly do a video on focus stacking with the images from the microscope please.
for taking videos the limiting resolution of the camera is given by the rate and time of transmitting pictures to memory and not by lenses or objects
Do you have any opinions on the nikon CF system? Where they compensate for all of the aberrations in the objective allowing for prime focus microphotography.
ok the phone mount can you explane it more were to get and such
Hi Oliver, thanks for your super informative videos! I just got a couple new set ups (trinocular Amscope T409b-dk, and trinocular stereoscope) to use with my mirrorless dslr and dedicated hayear scope cam. While my videos come out amazing, my still images are complete crap LOL. Do you need to really turn up the illumination in order to get acceptable shutter speeds for still images? I had to crank my iso to 1600 or 3200 to get just 1/30 of a second, which as you know isn't fast enough to negate motion blur, and the noise at those ISOs is frustrating. If you have to increase the light intensity, do you still use the eye pieces to follow your subjects or do you do everything on the screen of the camera? I have my cameras connected to a large 1080p television but its difficult to follow the subjects with any magnification over 10x, because my camera is an APS-C the field of view is fairly small. Thank you for any help you can lend me! Its difficult starting out ;o)
Great video! Many Thanks! Did you provide a video for the clamp?...or can you provide a link for the purchase site? Thanks!
Do you recommend or have a video on any of the pocket microscopes that attach to a phone?
The issue with the USB 3.0 dropping to 2.0 is the bandwidth of the USB bus. I'd similar issue while setting up my VR sensor, getting 4x USB 3.0 to work at the same time is a huge hassle if you have a VR ready motherboard.
TLDR; get a motherboard or PC with proper USB 3.0, or one that says VR ready.
which camera do you recommend for using a mac book as the monitor for darkfield blood studies?
Where can I buy the adapter thing starting at 11.08 to put digital camera etc
Have a trinocular microscope with LCD and HDMI camera but the LCD does show the image of the object
I liked the concept of the video, but the example videos towards the end were overlayed by youtube's "next video" popups so maybe should've had your end credits at the end to extend the video a little or put this further ahead into your video. I think this subject would warrant more videos that exemplify the imagery captured by different camera options (including using a cell phone adapter, DSLR, etc.) thanks for your content.
4:29 did you by chance try updating your bios ( aka flashing your bios) software?
One very important aspect of choosing a microscope camera is th CMOS SENSOR SIZE... Not just the megapixel count...
1/3.2" CMOS sensor = 7.9 mm (diagonal) FIELD OF VIEW (bit annoying discrepency between eye field and camera image field of view)
1/2.3" CMOS sensor = 11.0 mm (diagonal) FIELD OF VIEW (bit annoying discrepency between eye field and camera image field of view)
1/2" CMOS sensor = 12.7 mm (diagonal) FIELD OF VIEW
1/1.8 CMOS sensor = 14.1 mm (diagonal) FIELD OF VIEW
2/3" CMOS sensor = 16.9 mm (diagonal) FIELD OF VIEW (IDEAL for most microscope)
1" CMOS sensor = 25.4 mm (diagonal) FIELD OF VIEW (too big for most microscope)
Excellent, very helpful, thank you for posting it. If there is a way to, I would like to email you??
Thank you for very good video, can I asc what do you think about these 2 sensors one has 1080P/Sony IMX236 1/2.8"(5.5x3.4) the other is also Sony Imx185 1/1.9"(7.20x4.05) there is difference in size of the sensors which do you think is bether situable for trinocular stereo microscope to obtain good quality picture and field of view? (I have already 2 reduction lenses 0.35x and 0.5x)
Thank you.
Well, much depends on other factors as well, besides sensor size. But larger sensors have the advantage that one is able to simply see a larger area (larger field of view). Larger sensors also have a better signal to noise ratio. But with appropriate reduction lenses one can also get a better field of view with small sensors. If you cram a lot of pixels into a small sensor, then you might not fully benefit from the resolution, as you might get more "empty resolution", where the sensor has more pixels than the resolution that the microscope is able to supply.
My Chinese Omax/Novel binocular scope should arrive in a few weeks. I am thinking of getting a set of PLAN achromatic objectives (4x/10x/40x/100x) for about USD103. Is this a smart move, or should I not expect much improvement with Chinese PLAN objectives over the regular Chinese achromatic objectives that come with the microscope? I expect that these PLAN objectives are very similar, if not identical, to the Amscope PLAN objectives that get good reviews on Amazon.
In your experience, which camera adapter is best for taking pictures of findings without any movement from the camera inside the eye-piece? I'm looking for a camera that will remain completely still when I take pictures.
Hello,
Thank you for the informative video. I have a question, please. What do you think about the Nikon microscope that already has an assembled camera system built-in?
Thanks
Can you tell me the model of the microscope so that I can have a look at it? Normally high-end microscopes like Nikon etc do not have built-in camera systems (unless for specific uses), becasue they keep microscopes modular and for this reason they do not build in cameras normally.
Be careful with built in cameras... first if the camera experiences a problem you cannot simply send in for repair or replacement - at a minimum the entire binocular tube must go meaning your microscope is down...second they don’t allow for future camera upgrades or replacement like a phototube/camera would...a good microscope will last you decades but camera do not - even if you just consider operating system compatibility...in 15 years your microscope will hopefully be functioning perfectly fine but will the camera be compatible with current version of windows?...will much more powerful and inexpensive cameras be available that you might want to upgrade to? What if the camera fails - no one repairs cameras so you’re stuck...manufacturers move on from these camera models very quickly...so for these reasons I would generally not recommend built in cameras - even if at the time they are a slightly cheaper option
Apart from the compensating camera eyepiece and it corrections, would you have the same imagequality if the camera was mounted on one of the eyepieces?
The light is slit up between phototube and eyepieces, so there should be the same quality. I have a stereo microscope that uses mirrors to redirect the light to the phototube and I think that the quality though the phototube is actually a bit worse than though the eyepieces. So it depends mostly on the optics used.
Good point about not all computer manufacturers implementing USB 3.0 properly. Is there a way to fix this problem with firmware/software/driver, or is it a hardware problem?
Tried all but not successful. Will switch computers and try.
I am coming to like your vids and am becoming fascinated with the "undiscovered country" in my backyard garden. Can you suggest a scope that can resolve the kind of structures that I encounter, like mildew/mold and other water borne pathogens? I grow variety of veggies and my curcubits face threats like Phytophtora Capsici and Psuedoperonaspora Cubensis. By the way I LOVED your vids on Diatoms and now know why Diotomaceous earth kills cut-worms! Diotom shells are ciliicite and soft skinned insects take critical damage when they crawl thru it. Super! Thanks.
Hi @microbehunter!
You´re videos are awesome!
But seeing them, i have a question: i have a Sony A6300, a Aps-c cámera. I want to buy one microscope and the other stuffs to adapted it.
Could you recommend to me something please?.
I don´t have a lot of money :/
20:53 Can we simply use an eye piece of 5x (instead of the regular 10x) to get a similar image with the APS-C sensor Vs full frame? Or use a landscape zoom, wide angle, on the APS-C?
What would be the general effect?
I suspecte a wider field of view and smaller details with 5x ep + APS-C. I'm I right doctor?
Hi, what is the name of the clamp for the compact camera? Where can I buy it?
www.teleskop-express.de/shop/product_info.php/info/p219_digiklemme---universelle-befestigung-der-digitalkamera.html
or from amazon:
www.amazon.de/TS-Optics-Kameraadapter-Befestigung-digitalKamera-Mikroskopen/dp/B007CVTDIU/ref=sr_1_1?ie=UTF8&qid=1531083567&sr=8-1&keywords=digiklemme
It's from a German telescope retailer that manufactures it.
@@Microbehunter what eyepiece would I need to see live microscope sample? Like if I have my sample set on the microscope and instead of seeing from the microscope I would be able to see from the laptop
I have a microscope with monocular head (only one eyepiece). I would like to upgrade the head to a trinocular head. How can I tell if a trinocular head is compatible?
Hi. I have mikroskop . I need a microscope camera, what do you offer
Is there a reason to go for for an DSLR (Digital Single Lens Reflex) Camera over a MILC (Mirrorless Interchangeable Lens Camera) in regard of using it with a microscope?
I'm intersted in getting an answer to this question as well. Thank you!
Actually mirrorless cam works great for photomicrography. I just restored my American Optical AO One Sixty vintage microscope with the Sony A6000 attached (homemade mount). It works! Image way much better than my old Nikon D90 DSLR. Most of all, the mirrorless body is small and very light so it occupies less stress on the eyepiece barrel.
Couldn't you use reduction lenses to overcome the problem with the small fov on a small sensor?
Yes, and this is indeed done most of the time. In the case of the USB 3 camera, they use a different strategy by moving the sensor forwards to the focal point of the objective. This improves the situation a bit (compared to having the sensor further back) but still results in a relatively small fov.
May I ask what photo tube should I buy from ebay？I bought a second-hand Olympus CH30 with 3 eye yesterday，thank you
I would buy a low cost microscope camera that you can connect directly to the microscope without separate adapter. The microscope eyepiece cameras can be put into the microscope directly on the tube (or an eyepiece). If you want an original OM Phototube and NFK photo ocular from Olympus, then this can be expensive (if you even find one on ebay) and then you need an SLR camera as well.
thank you very much :-)
Sir you could have used the actual results with the settings used to demonstrate.
I meant to say the "clamp for focal photography using a digital camera (next to last set up shown on this video). Thanks
More info here (in German, though. Use Google translate):
www.teleskop-express.de/shop/product_info.php/info/p219_digiklemme---universelle-befestigung-der-digitalkamera.html
btw USB2.0 enough to transfer FHD@30fps - not too bad...
Thanks for the video, very helpful.
Should you not fix the title to "How can one take 'pictures' with a microscope?"
thanks, fixed it.
wo rechne ich falsch...Nehmen wir an ich mikroskopiere mit NA 1 ca 60fach. Dann liegt meine Aufloesung etwa bei 1 Mikrometer. Das reele Zwischenbild hat die Groesse von etwa 1 Quadratzentimeter. Dann benoetige ich bei 1my Aufloesung schon 10 hoch acht Pixel. Das bedeutet eine Kamera mit 100 Megapixel. Noch nicht beruecksichtigt dass der Sensor der Kamera quadratisch ist und das mikroskopische Bild rund
Does someone know a tutorial for adapting the exposure & gain in the microscope camera? Thank you!!!!
Do you still need help with that?
@@ThatGuy-bx4yi Yes please! it will help me a lot!!!
@@ilm5929 Are you using a USB camera for your microscope?
@@ThatGuy-bx4yi Yes, I have a OMAX microscope, and a OMAX USB camera. The camera has 10MP. But, the problem is that when I try to take pictures they are not that good.
@@ilm5929 have you heard of the program called OBS?
So after 4 years... simple solution exist?
Worth just get USB3 adapter?
EDIT: I answer myself.. If you don't have camera, and you want amazing results (video/images)
Just get modified GoPro camera from back-bone website. Example.. Gopro8 becomes compatible with M12, CS-Mount & C-Mount
And exactly C-mount adapter allows to connect a camera to the trinocular port of the microscope.
Just the info I needed. Thx. In case you're interested, "This is how it looks like" can just be shortened to "This is how it looks." That would be normal English usage. ;-)
How good is the quality of 350T ? is this clear enough for seeing Bacillus subtilis, Lactobacillus spp, Trichoderma spp, Bacillus spp and others ?
meanwhile china microscope not good enough
Of course you can see them (if you know what to look for or if you put a pure culture under the microscope). But this is not a quality question. To see bacteria better, you need phase contrast microscopy.
How about recording
I use an astrophotography camera (asi1600mm-cool) camera and have to put the camera without any adapter on my Amscope T490B microscope. I have usb-3 but am only able to record short movies due to limits of my laptop. Here is a movie of microbes under the microscope: th-cam.com/video/v-9prcG0BWs/w-d-xo.html
That looks pretty cool :)
There is a kit to turn a Logitech Brio 4K camera (4k in 30fps with USB3) into a CS mount enclosure. Do you think that would be a good way to go?
Have you looked into 3D printers? It should be easy to make great adapters with a 3D printer. Or even 3D print a full microscope.
I thought about it, but I am not sure if the 3D printers have the resolution to make a sufficiently fine threading for screws etc. Or maybe the plastic is soft enough and a threading is not required. 3D printing a full microscope might only be meaningful if the microscope is 3D printed from metal (laser heat to melt metal?). Plastic might be too light to be stable, unless one adds weight. And also here there is the question of tolerance and mechanics. The Legoscope does use 3D printed parts: legoscope.squarespace.com/
This one is similar to the one I made (which does not use 3d printed parts).
Geez. You need sharpcap pro and a dedicated astrocam like asi178mc and a uv/ir block filter.
you should number your content with clickable information at different time stamps
the heavy and clumbsy DLSR cameras are obviously not the best solution for microscopie except that you can use them for normal fotos. Shurly microfotografie today makes no sense because we can make excelent moovies, but not only with a DLSR
So we know what NOT to buy! And the adapter tube is "not cheap" even used. Try $1000+
So none of the smaller self contained digital eyepieces are worth getting?
you forgot the simplest way.....drawing
what a senseless calculation and discussion.......the human eye has a resolution of max 4.1 Megapixel. The limiting factor ist the resolution of the presentating media. Use a Laptop ur Desktop with heigh resolution: 1920 x 1080 and that is about 2 Megapixels,. So an camera or 3 mega is sufficcent
Dear Lothar, a human eye has near 130 megapixel (Wikipedia). So, please, do not think only you are wise and others are stupid. You obviously are not a microphotographer.
@@KraterStromboli only percent of 130 megapixel are used, the others are not sharp. I am no microphotographer but a scientist
@@lotharmayring6063 I am also a scientist and use photography above 50 years with telescope and microscope. The main task was to get the image of maximum resolution and detailed.
A 35mm film frame (24×36mm) has 12 Mpx.
From which reliable sources on good authority have you got that yours percent?
@@KraterStromboli the resolution of the best optical microscope objektiv is less than 3Mpx. Why do you not use electron microscope or space telescope, it seems to be a hobby for you, have a look at wikipedia
@@lotharmayring6063 resolution of optical devices is measured in line per mm. Pixel for matrix or film. Resolution of microscope depends on monochrome short wave illumination (ultraviolet and quartz optics). So, yours information about microscope objectives is inaccutate, regrettably.
too top heavy not desinged for the job
Would you happen to have a link for the adapter that you used at 10:36 or something similar?