This is a better start than I had last time, 100% on the quizzes but we'll see how I fare in the harder upcoming material before I get too bold. I absorbed the graphs better this time (but not adsorbed)
I've heard you mention plaque assays so often before, but I didn't realize how analogous the process is to serial dilution and quantification of CFUs in bacteria - but now I do! way cool
I was quite surorised that in his 2023 lecture Pr Racaniello gave his firm opinion that the origin of Covid19 is non a lab leak but a natural origin. For precise scientific iformation about the covid 19 origine, there is an excellent book VIRAL by Dr Alina Chan and Matt Ridley PhD. And also a lot of videos of these 2 authors, many serious info, very well sourced.
Putting unfiltered BALF from a sick person onto mono-layer cell-culture in a plastic Petri dish and then observing effects over time demonstrates/replicates what natural observable phenomenon? i.e. how in the world can you claim that process has anything to do with finding [insert your definition of a virus here]?
Thank you for this awesome primer. I've recently spent some very interesting time diving into some rabbit holes re: the virally caused alpha/gal reaction. I'm especially interested in the appearance of symptoms of the arthropod saliva reaction being similar to corona virus ones. A random (?crazy) question that also occurs to me as I watched this video, is that, when a magnetic field is introduced, as in "spinning" the cell solution, is the spin not creating an electrical field? And, would that field's charge affect the properties and consequent ionic interactions of those cells?
Magnetic fields can affect ions both in solution and in vacuum tubes (that latter is the basis of ion mobility mass spectrometry), however magnetic stirrers utilise ferrite magnets attached to a motor, and stirrer bars are teflon coated ferrite magnets. Hence the magnetic field is rather weak, and while it would have a weak effect on charged species, it unlikely to affect the cells.
Thank you!!! Well I never! I doubt I understood a word, except that PCR measurements are not the same as infectivity! But I have heard of Shi Zheng-Li of the WIV. Wikipedia has an interesting introduction into her life and work.
Some viruses cause plaques in cell culture and others do not. Plaque formation does not predict the ability to cause disease in animals. Nevertheless, among the viruses that are pathogenic and do cause plaques, infecting animals with more PFU's will cause greater disease.
So I know this might sound kinda dumb and was probably explained already but when a virus can’t enter the cell would that person be able to pass the virus to another person even though the virus hasn’t entered any cells?
Hmm, that's a good question. I'm still learning myself but I guess it depends if the Virion is preserved and stable in whatever part of the body it finds itself in
One egg’s worth in one influenza shot? No wonder my egg anaphylactic kid reacts so allergically to the influenza shot. We haven’t gotten it since. Our pediatrician says there should be no reactivity to shots grown in eggs even for egg anaphylactic people. What have you heard?
There are some flu vaccines produced by egg-free methods, this will vary country by country so you can research your own region to see what is available. Vincent has mentioned this elsewhere.
Immuno compromised patients are virus factories. A large number of mutated variants come from them because their immune systems never clear the virus properly.
Some Cancer probably is a ( virally) initiated change in the normal cells program. Henrietta's cancer was an HPV virus that had lost it's program to allow malformed cells to be destroyed.
There is still a lot of controversy over whether or not the source of the virus was a lab or wet market. You show proof from the Chinese lab workers. Do you have proof from any other source or do you blindly follow what the Chinese say? I don't. This is a big thumbs down and unscribe!
I'm so glad that you have made these gems available online, Dr. Vincent! Many thanks to you!
As a non scientist, I have to say I love your lectures.
Thank you! Great primer to help understand what's important!
This is a better start than I had last time, 100% on the quizzes but we'll see how I fare in the harder upcoming material before I get too bold. I absorbed the graphs better this time (but not adsorbed)
Me too. Yep😉
I've heard you mention plaque assays so often before, but I didn't realize how analogous the process is to serial dilution and quantification of CFUs in bacteria - but now I do! way cool
Really great editing this year! Congrats to the editors.
The slides are really helpful. I copy most of them.
I was quite surorised that in his 2023 lecture Pr Racaniello gave his firm opinion that the origin of Covid19 is non a lab leak but a natural origin. For precise scientific iformation about the covid 19 origine, there is an excellent book VIRAL by Dr Alina Chan and Matt Ridley PhD. And also a lot of videos of these 2 authors, many serious info, very well sourced.
Racaniello and others have debunked this. Watch any of the multiple TWIV episodes he's done on this.
You make it sound so easy
time flies, it is already 2024, Vincent 😂
Love these lectures
Lecture 2 - Welcome Back 2024!
Enjoy Your Day! Be Safe & Stay Encouraged!
Thanks! 👏👏👏
Putting unfiltered BALF from a sick person onto mono-layer cell-culture in a plastic Petri dish and then observing effects over time demonstrates/replicates what natural observable phenomenon? i.e. how in the world can you claim that process has anything to do with finding [insert your definition of a virus here]?
Thank you for this awesome primer. I've recently spent some very interesting time diving into some rabbit holes re: the virally caused alpha/gal reaction. I'm especially interested in the appearance of symptoms of the arthropod saliva reaction being similar to corona virus ones.
A random (?crazy) question that also occurs to me as I watched this video, is that, when a magnetic field is introduced, as in "spinning" the cell solution, is the spin not creating an electrical field? And, would that field's charge affect the properties and consequent ionic interactions of those cells?
Magnetic fields can affect ions both in solution and in vacuum tubes (that latter is the basis of ion mobility mass spectrometry), however magnetic stirrers utilise ferrite magnets attached to a motor, and stirrer bars are teflon coated ferrite magnets. Hence the magnetic field is rather weak, and while it would have a weak effect on charged species, it unlikely to affect the cells.
@@Costa_Conn thank you for your explaition.
Thanks!
I really appreciate this course. nevertheless, can i have pdf file of the lecture please? it would be really helpful for study
It might be available on his site, if you look up Racaniello's Virology or TWIV
Thank you!!!
Well I never! I doubt I understood a word, except that PCR measurements are not the same as infectivity!
But I have heard of Shi Zheng-Li of the WIV. Wikipedia has an interesting introduction into her life and work.
where we find notes of these lectures
Thanks.
Thank you so much
Do we know that there is a positive correlation between plaque forming ability and actual infectivity in animals?
Some viruses cause plaques in cell culture and others do not. Plaque formation does not predict the ability to cause disease in animals. Nevertheless, among the viruses that are pathogenic and do cause plaques, infecting animals with more PFU's will cause greater disease.
At minute 20: the form used to indicate the spike proteins should be different. They look like antibodies which is a little confusing.
So I know this might sound kinda dumb and was probably explained already but when a virus can’t enter the cell would that person be able to pass the virus to another person even though the virus hasn’t entered any cells?
Hmm, that's a good question. I'm still learning myself but I guess it depends if the Virion is preserved and stable in whatever part of the body it finds itself in
One egg’s worth in one influenza shot? No wonder my egg anaphylactic kid reacts so allergically to the influenza shot. We haven’t gotten it since. Our pediatrician says there should be no reactivity to shots grown in eggs even for egg anaphylactic people. What have you heard?
There are some flu vaccines produced by egg-free methods, this will vary country by country so you can research your own region to see what is available. Vincent has mentioned this elsewhere.
What do you think about California has overridden isolation in their state to 1 day without fever. Period
People need to have lower immunity to make virus cycles great again.
Immuno compromised patients are virus factories. A large number of mutated variants come from them because their immune systems never clear the virus properly.
15:30 Cancer is the pathway to immortality. That doesn't sound right.
Some Cancer probably is a ( virally) initiated change in the normal cells program. Henrietta's cancer was an HPV virus that had lost it's program to allow malformed cells to be destroyed.
@@roncarlin3209 ironically, it is!
There is still a lot of controversy over whether or not the source of the virus was a lab or wet market. You show proof from the Chinese lab workers. Do you have proof from any other source or do you blindly follow what the Chinese say? I don't. This is a big thumbs down and unscribe!
Stop falling for sensationalized fearmongering and do some research. Racaniello and others have debunked these claims in multiple episodes of TWIV.