Im still trying to find out where the word digital means in silico or does it actually involve wet lab - molecular hands on work? Digital is misleading if that is the case.
It does involve wet lab. The "digital" moniker is based on the on vs. off from the signal seen per well. That is, when a single well has the target, it produces a signal while, a well without the target remains unlit. This is akin to the on/off in digital signals. I hope this helps.
Like digital cams, many kinds of detectors are used in qPCR:CCD CMOS,even PMT. You can find which kind of detector is used in your instrument User Guide.
I am doing relative gene expression in different tissues spleen, lung etc Should I have to run reference control for each tissue. Or one reference control is enough?
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"clout"
yeah clout isn't a new word as a lot of people may think nowadays
Please Tell me how can i calculate T/S ratio for Telomere length
Im still trying to find out where the word digital means in silico or does it actually involve wet lab - molecular hands on work? Digital is misleading if that is the case.
It does involve wet lab. The "digital" moniker is based on the on vs. off from the signal seen per well. That is, when a single well has the target, it produces a signal while, a well without the target remains unlit. This is akin to the on/off in digital signals. I hope this helps.
Which of detector is used to detect excitation of dyes or probes in qpcr
Like digital cams, many kinds of detectors are used in qPCR:CCD CMOS,even PMT.
You can find which kind of detector is used in your instrument User Guide.
I am doing relative gene expression in different tissues spleen, lung etc Should I have to run reference control for each tissue. Or one reference control is enough?
Thank you for your question. Please contact our technical support team at thermofisher.com/askaquestion for additional information.
Thank you!