How are biotinylated custom baits designed when the sequence of the DNA to which it will bind to is not even known? and if we already know the sequence of the target region then why are even sequencing it??
It is mostly used for studying objects that are expected to be related to the objects we know enough about, like if scientists are making a research on ancient horses - they can enrich those ancient DNA libraries with modern horses' specific genomic regions. Working with mitochondrion is even simipler - mitochondrias are quite conservative so if you want to specifically get info about some species mitochondrial genome you can enrich it with even a distant modenr species (it was proved by my lab that you can enrich ancient bear mtDNA with a modern sheep mtDNA)
For that adapters and linkers might be used to ligate it with our target dna and then probes can be designed according to the sequence of linker / adapter used.
If we want to create an animal model for a disease, we mutate its genes. But to make sure that mutation actually occurred, we want to do sequencing. Also, we use it to compare different genomes with each other. For example, if i want to see mutations in breast cancer patient, i will take his/her sample from them, sequence them, and then compare them with normal humans.
Thank you for the video. Can you please tell me the maximum length of the fragment that can be captured with this technique?
How are biotinylated custom baits designed when the sequence of the DNA to which it will bind to is not even known? and if we already know the sequence of the target region then why are even sequencing it??
It is mostly used for studying objects that are expected to be related to the objects we know enough about, like if scientists are making a research on ancient horses - they can enrich those ancient DNA libraries with modern horses' specific genomic regions. Working with mitochondrion is even simipler - mitochondrias are quite conservative so if you want to specifically get info about some species mitochondrial genome you can enrich it with even a distant modenr species (it was proved by my lab that you can enrich ancient bear mtDNA with a modern sheep mtDNA)
Ours use known sequences for known targets. So you can for example screen for a pathogen if you know its sequence
For that adapters and linkers might be used to ligate it with our target dna and then probes can be designed according to the sequence of linker / adapter used.
If we want to create an animal model for a disease, we mutate its genes. But to make sure that mutation actually occurred, we want to do sequencing.
Also, we use it to compare different genomes with each other. For example, if i want to see mutations in breast cancer patient, i will take his/her sample from them, sequence them, and then compare them with normal humans.