Dear Brianna By " fluke " I came across your videos and I am overwhelmed. Then when I look at your playlists, they almost intimidate me. Sincere thanks for your immeasurably valuable efforts and generosity in imparting your knowledge in a sympathetic and competent manner. Great respect! Greetings from Switzerland
Theory Note: You have to go really fast to pull out small suspended particles because you need to generate enough centrifugal force to counteract the buoyant forces and the friction opposing the particles’ downward movement. The sedimentation rate for a particle depends on its size, its density compared to the density of the media, and the media’s viscosity (goopiness). If you want to learn more I recommend this guide: www.fishersci.se/se/en/scientific-products/centrifuge-guide/centrifugation-theory.html#tab2 Here’s a nice guide to what types of speeds/forces* you need to pellet out various things: www.sigmaaldrich.com/US/en/technical-documents/technical-article/protein-biology/protein-pulldown/centrifugation-basics The centrifugal force (the ref, or “g-factor”) depends on both the speed of rotation (rpm) and the rotor diameter. More here: blog: bit.ly/lab_centrifuges; TH-cam: th-cam.com/video/xCu7xDfyBBw/w-d-xo.html You can use gradient centrifugation, increasing the viscosity and density of the media if you want to separate even smaller things - more about that in the links above.
more (hopefully) helpful random practical lab tips & tricks: bit.ly/lab_tricks_page
more about all sorts of things: #365DaysOfScience All (with topics listed) 👉 bit.ly/2OllAB0 or search blog: thebumblingbiochemist.com
Dear Brianna
By " fluke " I came across your videos and I am overwhelmed.
Then when I look at your playlists, they almost intimidate me.
Sincere thanks for your immeasurably valuable efforts and generosity in imparting your knowledge in a sympathetic and competent manner.
Great respect!
Greetings from Switzerland
Thank you so much for your kind words. And please don't feel intimidated! Biochemistry is awessome!
@@thebumblingbiochemist Indeed! Not intimidated by biochemistry, but by your work 😂❣️
Theory Note: You have to go really fast to pull out small suspended particles because you need to generate enough centrifugal force to counteract the buoyant forces and the friction opposing the particles’ downward movement. The sedimentation rate for a particle depends on its size, its density compared to the density of the media, and the media’s viscosity (goopiness). If you want to learn more I recommend this guide: www.fishersci.se/se/en/scientific-products/centrifuge-guide/centrifugation-theory.html#tab2
Here’s a nice guide to what types of speeds/forces* you need to pellet out various things: www.sigmaaldrich.com/US/en/technical-documents/technical-article/protein-biology/protein-pulldown/centrifugation-basics
The centrifugal force (the ref, or “g-factor”) depends on both the speed of rotation (rpm) and the rotor diameter. More here: blog: bit.ly/lab_centrifuges; TH-cam: th-cam.com/video/xCu7xDfyBBw/w-d-xo.html
You can use gradient centrifugation, increasing the viscosity and density of the media if you want to separate even smaller things - more about that in the links above.
more (hopefully) helpful random practical lab tips & tricks: bit.ly/lab_tricks_page
more about all sorts of things: #365DaysOfScience All (with topics listed) 👉 bit.ly/2OllAB0 or search blog: thebumblingbiochemist.com
#scicomm #biochemistry #molecularbiology #biology #sciencelife #science #realtimechem
Grewat video!, what type of centrifuge tube is that you are holding at the start of the video?
Thanks! That's for the ultracentrifuge Ti45