I really appreciate this videos, thank you very much Alex. One question, how do you know the illuminaclip that you nedd when you download or receive fastq data? Thanks again
Hlew sir if i want to trimm data before allignment.. For example if i take 2000 covid data from server and want to align it. Then what should i do first to trimm those 2000 data and then i can run the allignment and use it for future works. Or can i align data without trimming will it be significant? Thanks in advance sir
@@alexsoupirThanks Alex, I found you have experience with microbiota from your GitHub, so, I was asking because most people that got 16s data from our university use trim Galore, while I used trimmomatic before for RNAseq so thought why use a new tool for trimming. Cheers Marwa
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Thank you so much for the help. I was struggling with understanding the parameters and now I understand the basic concept.
Gracias. Este video es muy bueno. Que bueno eres explicando el proceso
very nice work done Alex, hope to see more videos:)
Thank you Alex
Thank you so much for the lesson.. However, I didn't quite get the concept of paired and unpaired output files. Can you please elaborate?
I really appreciate this videos, thank you very much Alex. One question, how do you know the illuminaclip that you nedd when you download or receive fastq data? Thanks again
Hlew sir if i want to trimm data before allignment.. For example if i take 2000 covid data from server and want to align it. Then what should i do first to trimm those 2000 data and then i can run the allignment and use it for future works. Or can i align data without trimming will it be significant?
Thanks in advance sir
Do you recommend using trimmomatic for quality control check of microbiota (16s rRNA)?
That would depend on the methods used for sequencing but usually it you send got just 16S you wouldn't need to.
@@alexsoupirThanks Alex, I found you have experience with microbiota from your GitHub, so, I was asking because most people that got 16s data from our university use trim Galore, while I used trimmomatic before for RNAseq so thought why use a new tool for trimming.
Cheers
Marwa
just like the phred score my understanding decreased over time :)