Assalamoalikum sir. I am using new c18 colum in shimadzu hplc. I washed with 100 percent methanol for 20 mint with f.r. 0.5 and 1.0 ml Then washed with hplc h2o for 20 mint with 0.5 and 1.0 flow rate
Then I run filtered mob phase and check the b.line for 15 mint. After I inject my sample of conc 10 ppm. The chromatogram showd the solvent peak with arrea and height. But my active ingredient peak not showed or heightened
Manzoormemon is same. I m saranoor sir.this is 2nd id
Previous column company is teknokroma
New column company is beckmancoulter
Then don't change the column company
Assalamoalikum sir. I am using new c18 colum in shimadzu hplc.
I washed with 100 percent methanol for 20 mint with f.r. 0.5 and 1.0 ml
Then washed with hplc h2o for 20 mint with 0.5 and 1.0 flow rate
Column should be washed first with water then with methanol ,if buffered mobile phase used.
Walaikum salam
The configuration of previous column is SEA 18 5um 25×0.46
The configuration of new column is UL TRASPHWRE
ODS
4.6 mm × 25 cm
This is because of new column choosen....
So done change anything in column use new column same as previous
I am worry.
Actually before New colum.
I runed my sample on another column there were my concerned peak but on new colum there is nothing no height
Check the column configuration and company or brand name ,, same or not
Previous column. Company is teknokroma
New column is beckmancoulter
Sometime new column also create problem, so plz change the column
Then I run filtered mob phase and check the b.line for 15 mint.
After I inject my sample of conc 10 ppm. The chromatogram showd the solvent peak with arrea and height. But my active ingredient peak not showed or heightened
Check column configuration means company name and brand for previous and for new one
Sir how can I check column configuration
Sir is se kia hoga
I m worry