Great video Mr. Mayne. Very good presentation. A few suggestions. 1. For gelling agent you can use - KIRKLAND SIGNATURE NATURAL PSYLLIUM FIBER 2. If you add 5 mg/L of Copper Sulfate Pentahydrate (buy from Home depot or such stores) - (You take a 5 grams dissolve in 1 litre to form Mother solution and add 1 ml of it to your 1L media). you will have more shoot and root development and less chances of fungal, bacterial infections.
@@AquaMayne Not only that, since CuSO4 kills off any Fungus or Bacteria it diverts more energy to shoot and root multiplication. In most plants (value of best ppm differs) the number of shoots and roots is almost double when compared to no copper used control bins. You can look up some examples of Banana tissue culture and others in Google search.
@@bijanmozafari3826 Most of them gets benefit from CuSO4.5H2O. It is better to use 1 ppm to be on safe side. Do not discard remaining solution in small water bodies as it can cause microbial life to collapse.
Mr Mayne! The timing of this video is unbelievable. I just discovered part 1 today and didn't have to wait like the other tubers for part 2. Really great content. Thanks a bunch.
😍😍 STILL stalking all your vids....dude, these are great instructional vids! 273 thumbs and almost 10K views...very nice. You rock...keep the content coming! 😍😍
Great vid, thanks for sharing... I've curiosity to know how long it takes to get a plant from when u plant it in that media compared to just plant a little trim direct in the tank?
Great video man! it's a fun experiment, but I think it is too much work for just a common plants.. what you should tissue culture are many bucephalandra species, aponogeton madagascar, and anubias. Those are much more rewarding for a tissue culture propagation
Is there any special procedure for aquatic plants? Do they need to be transitioned out of water in stage 0 and do they need to be transitioned into water in the final stage? Do they do just as well as regular plants in the multiplication media? Thanks in advance.
Yes that is the basic order of TC. Some people skip rooting though. Obviously you will not have as many roots for the plant to establish outside of TC though if rooting is skipped.
@@AquaMayne Would something like this work? www.ebay.com/itm/Plant-Tissue-Culture-Media-Rooting-IBA-NAA-Microclone-Clone-Cloning-TC-215-420/223887580780
Where do I buy Media, Plant hormones (BAP, IBA) and PPM ? Tried Caisson but they dont have delivery to Canada. Can you kindly provide any supplier link?
@@AquaMayne cool man, thank you for the great videos, they kinda filled in some missing gaps in information that is not super easy to come by.....got me up and started, and now im hooked, mostly using it for cannabis but im attempting some aloe, christmas cactus, coleus, my bonsai tree, any house plant i can get my hands on lol....thank you for taking the time....i linked your videos on a thread in a forum if you dont mind....dont worry im not taking credit for any of it, just want to pass the info on to more people.
Merci pour cette vidéo qui va bien m’aider même si du coup votre méthode n’est pas entièrement stérile je vais utiliser le même procédé mais avec une glowe box faite maison afin d’être sûr de travailler en milieu stérile
Is it too early for an update? I'd love to see the progress of your plant cultures.
This is exactly the kind of video I’ve been looking for. So elaborate and everything makes sense!
Great video Mr. Mayne. Very good presentation. A few suggestions. 1. For gelling agent you can use - KIRKLAND SIGNATURE NATURAL PSYLLIUM FIBER 2. If you add 5 mg/L of Copper Sulfate Pentahydrate (buy from Home depot or such stores) - (You take a 5 grams dissolve in 1 litre to form Mother solution and add 1 ml of it to your 1L media). you will have more shoot and root development and less chances of fungal, bacterial infections.
Thanks for the tips, so the copper sulfate is basically a plant preservation liquid?
@@AquaMayne Not only that, since CuSO4 kills off any Fungus or Bacteria it diverts more energy to shoot and root multiplication. In most plants (value of best ppm differs) the number of shoots and roots is almost double when compared to no copper used control bins. You can look up some examples of Banana tissue culture and others in Google search.
That's correct
@@Shyam-ym3em good info. is this proven across the board for all plants in micropropagation?
@@bijanmozafari3826 Most of them gets benefit from CuSO4.5H2O. It is better to use 1 ppm to be on safe side. Do not discard remaining solution in small water bodies as it can cause microbial life to collapse.
Awesome video!
Thank you for explaining and showing it all in great detail.
Mr Mayne! The timing of this video is unbelievable. I just discovered part 1 today and didn't have to wait like the other tubers for part 2. Really great content. Thanks a bunch.
Great video ,I think this is the only video that is dealing with DIY aqua plants Tissue culture. keep updating
You are a great teacher
Thank you! 😃
hello. nice to see there's an effort for tissue culture.
im a date palm farmer.
can this method applied on my date palm?
Excellent tutorial!
😍😍 STILL stalking all your vids....dude, these are great instructional vids! 273 thumbs and almost 10K views...very nice. You rock...keep the content coming! 😍😍
Would love to hear about the progress
Best vdo on TH-cam
WOW really good content! this is very different than other peoples channel!!
Fish and plant thanks!
How did these TC babies go?
This is absolutely fascinating, i'm so excited to try this myself! :D
Great video! Any update on those 6 bottles that was done in this video?
Fantastic video!
Great vid, thanks for sharing... I've curiosity to know how long it takes to get a plant from when u plant it in that media compared to just plant a little trim direct in the tank?
tank is faster
Love this so much!!! Please make an update video! (:
I will!
thanks a lot for the video keep uploading the updates
Sure thing!
Enjoyed you video. What is the protocol to get the multiplied and rooted plants back into the aquarium? First a day is sterile distilled water?
Great video man! it's a fun experiment, but I think it is too much work for just a common plants..
what you should tissue culture are many bucephalandra species, aponogeton madagascar, and anubias.
Those are much more rewarding for a tissue culture propagation
Thanks for the tips!
Thanks! I was wondering why you grabed the plant after dipping in alcohol at 15:00
hello, if i want to propagate a plant like java fern or anubias, do i use a piece of rhizome or a piece of leaf and put it in the media
Rhizome would be more guaranteed but you can do a leaf if you use a medium with rooting hormone.
So....do we just remove that plant and put it in a rooting medium for the roots after a few months by following the same procedures???
Thank you for your very informative video.
Do we have to do the same washing process when transfer plants from multiplication media to rooting media?
No If you do it in a still air box or laminar flow hood you can transfer straight over very carefully
@@AquaMayne Thank you so much, waiting for my multiplication ones to grow and will try that.
did someone test the success rate with less sterilization steps?
you should probably use aluminium foil instead of paper towel...
Great video!!! 🙏🙏🙏
Is there any special procedure for aquatic plants? Do they need to be transitioned out of water in stage 0 and do they need to be transitioned into water in the final stage? Do they do just as well as regular plants in the multiplication media?
Thanks in advance.
So you make one set of media cups with multiplication media, and then transfer the plantlets into cups with the rooting media?
Yes that is the basic order of TC. Some people skip rooting though. Obviously you will not have as many roots for the plant to establish outside of TC though if rooting is skipped.
@@AquaMayne Would something like this work? www.ebay.com/itm/Plant-Tissue-Culture-Media-Rooting-IBA-NAA-Microclone-Clone-Cloning-TC-215-420/223887580780
Does isopropyl alcohol work?
i can not find the tubs what u used i have look on ebay amzon and whish
Hey Aqua Mayne, after 17:23 what liquid did you put inside those containers? just normal water?
Where do I buy Media, Plant hormones (BAP, IBA) and PPM ?
Tried Caisson but they dont have delivery to Canada.
Can you kindly provide any supplier link?
what's the last situation
hey where do you buy your supplies? media, hormones, etc....
please!
Ross Tafarian caison labs or phytotech labs
@@AquaMayne thank you sir!.....was able to find some medias on amazon to start me out but i will defanately check out your sources.
thanks guy!
@@AquaMayne sorry i missed that part in the first video...just saw it now.
Finally he is back
Waiting for 3 Month update!
Bro, my brain just exploded
you just touched the plant before putting it in the media
and don't tell me "my hand is sterile"
ever have any luck making a callus?
Ross Tafarian yes I made AR red calluses. I failed at multiplication after callus though
@@AquaMayne cool man, thank you for the great videos, they kinda filled in some missing gaps in information that is not super easy to come by.....got me up and started, and now im hooked, mostly using it for cannabis but im attempting some aloe, christmas cactus, coleus, my bonsai tree, any house plant i can get my hands on lol....thank you for taking the time....i linked your videos on a thread in a forum if you dont mind....dont worry im not taking credit for any of it, just want to pass the info on to more people.
@@mud3455 No Problem!
where is the results vdo?
No update in over 2 yrs So i'm guessing they died?
Most lived and added to the aquarium, my most recent vid is a laminar flow hood for TC in the future when I get time :)
We have the same stove lmao
Update???
Just built a fish room , focusing on that then will be doing some TC stuff in the future
Please upload the next vdo.
Merci pour cette vidéo qui va bien m’aider même si du coup votre méthode n’est pas entièrement stérile je vais utiliser le même procédé mais avec une glowe box faite maison afin d’être sûr de travailler en milieu stérile