@@KrisD007 ACD-A is a commonly used anticoagulant and is generally considered safe. However, the issue with vacutainers is not this ingredient. The issue is that vacutainers are not FDA cleared to create PRP and that they may contain endotoxins - according to the manufacturer of Vacutainers, BD: "BD does not claim that any of the Vacutainer blood collection tubes are endotoxin free." www.bd.com/en-ca/offerings/capabilities/specimen-collection/blood-specimen-collection/venous-collection/bd-vacutainer-blood-collection-tubes/vacutainer-blood-collection-tube-faq/general-tubes-faq#:~:text=(10%20boxes).-,Q.,collection%20tubes%20are%20endotoxin%20free.
The glass BD ACD-A Vacutainers (the ones he's holding) have been proven to not contain endotoxins. They have been repeatedly tested and verified in several published studies to be not only endotoxin free, but are ideal for even intra-articular PRP injections in which absolute sterility has to be maintained.
This 2004 study found endotoxins in glass BD Vacutainers: academic.oup.com/clinchem/article/50/11/2215/5640121 It's also worth noting that your confidence of BD ACD-A Vacutainers being endotoxin free is not shared by the manufacturer of these tubes. To quote BD's own website: "BD does not claim that any of the Vacutainer blood collection tubes are endotoxin free." www.bd.com/en-ca/offerings/capabilities/specimen-collection/blood-specimen-collection/venous-collection/bd-vacutainer-blood-collection-tubes/vacutainer-blood-collection-tube-faq/general-tubes-faq#:~:text=(10%20boxes).-,Q.,collection%20tubes%20are%20endotoxin%20free.
I'm not sure where there's any confusion - you can read the study we posted above your comment. A published, peer-reviewed study has demonstrated the BD Vacutainers were found to contain endotoxins. It's not a hypothetical matter - it's a documented fact.@@bballev2
'The 5-mL heparin Vacutainer Tubes (lot no. 3224585) were positive for endotoxin (10 ng/L), whereas another lot of 5-mL tubes (lot no. 4029101), 4-mL plastic heparin tubes, and 10-mL glass heparin tubes were negative for endotoxin (
sir plz tell centrifuge @ rate ,or how much time we have to centrifuge at what rpm single or double spin ? and how much sample of prp in ml is needed for 1 sitting ?
Is it illegal to inject PRP made with vacutainer (or any other vessel which is not FDA cleared as PRP kit) to patient in the US? Or is it just literally ‘not good for health’?
It is against FDA regulations (and therefore illegal) to inject PRP into a patient that was prepared in a device that has not been FDA cleared for PRP preparation. Additionally, it is not good for health to inject endotoxins (which are found in vacutainers) into a patient's body.
Very informative! Just a question: FDA approval is required only in the United States, correct? But I don't live in the US and I'm interested to see a dermatologist for PRP therapy for hair regrowth. I'm just watching a bunch of related videos to know more about the procedure before take that decision. But I'm a little afraid right now. How can I make sure that they will not inject those endotoxins on my scalp?
The FDA is a US-based regulatory agency and it's jurisdiction would not extend to countries outside of US jurisdiction. We can't advise on how to find appropriate medical devices outside of the US.
The way to make sure that endotoxins are not injected into your scalp is to call your dermatologist and ask what brands and models of PRP tubes they use. With some patience and encouragement they should provide you with that information. Once you have the brands and models that they use, research that info to find out if the tubes contain endotoxins or not. If they don't, make your appointment for the procedure. At the appointment, right before they are about to start the procedure, verify for yourself that they are indeed using the PRP tubes they said that they use that are endotoxin free.
Hello i have been asking loads but no answer good evening sir hope you are doing well. What sort of Blood Collection Tubes Can i put blood in and drink it from don't need a lot but i need to try this so i want to do it in a save way but no one will let me know im getting left to do this in a unsafe way please help many thanks take care
The word endotoxin is very scary, its essentially parts of cell walls of Gram negative bacteria that is often released into the surrounding environment. In infected tissues these endotoxin concentrations are extremely high and cause significant inflammation, might it be that endotoxins are left over in the tubes because they've been sterilized properly. Now if some endotoxin which is an proinflammatory substance not a bacteria, is left over enough to be detectable in the BD tubes then that makes sense since the tube was sterilized but does that mean it causes illness/disease, pretty unlikely considering the concentration of endotoxin required would need to be very high. Considering that in PRP not all the fluid is remove from the tubes for injection, only the platelet rich plasma which is less than 1/5th the total blood volume drawn, further diluting the endotoxins exposure. This is a scare tactic people, endotoxins can't cause problems unless in very high concentration as in the conditions that only infection can cause, if these tubes were not sterilized, which they claim to be then significant infections would result. The one study used as "fact" showed endotoxins present one Lot of BD vacutainers that had Sodium heparin additive which are not used for PRP, ACD-A is, are there any in ACD-A Yellow Top vacutainers probably but the only results would be inflammation which PRP treats! - Critical thinking suggests this video is hyperbole to sell a product. Yes, I'm a medical professional.
BD says on their website "BD does not claim that any of the Vacutainer blood collection tubes are endotoxin free." So it's their company admitting they have endotoxins in their tubes - not just a study. We suspect that most patients would prefer to receive PRP from medical devices that are FDA cleared for the preparation of PRP and that do not contain endotoxins. However, if as a medical provider, you feel comfortable using non-FDA cleared vacutainers, which contain endotoxins, to make PRP - perhaps you should mention your clinic name so patients know where to seek your discount services.
Most patients would prefer to receive PRP from medical professionals who use FDA-cleared medical devices that do not contain endotoxins an that are intended for making PRP. But maybe there are some thrill seekers who prefer providers like you who like to cut corners and do it the cheap way - even if it's not safe or federally compliant. Would you like to share your practice name in the comments so everyone can know where to find your unconventional services? Also, BD says on the FAQ on their website that all of their blood collection tubes can contain endotoxins. This is shown in the video and you can look it up on their website today. I'm not sure why you're trying to discredit this fact that is plainly available for all to see.
Nah, of course they have to sell. I work with surgeons and we use BD tubes for PPP eye serums. Are you saying we are putting endotoxins in our patients’ eyes? Lol. We use prp tubes from a different company for other eye and skin treatments too. So far, no complains of endotoxins from our patients cos our team of doctors could have known that, right?
Please share the name of the hospital you work for so we can help inform your patients that YES - you are likely putting endotoxins into your patients' eyes. BD says on their own website that all of their blood collection tubes contain endotoxins. Read for yourself on the 5th question on this FAQ: www.bd.com/en-ca/offerings/capabilities/specimen-collection/blood-specimen-collection/venous-collection/bd-vacutainer-blood-collection-tubes/vacutainer-blood-collection-tube-faq/general-tubes-faq "Q. Are BD Vacutainer blood collection tubes endotoxin free? A. No, BD does not claim that any of the Vacutainer blood collection tubes are endotoxin free." Sadly a lot of doctors do not understand the risks of illegally using blood collection tubes to make PRP. That's why we release educational content like this video. If the doctors at your clinic want to make PRP that is safe and effective for your patients, then they should use a medical device that is FDA cleared for the preparation of autologous PRP.
Seriously, surgeons have had enough education. They are very specific and careful when it comes to what they put in their patients’ eyes. I make the eye serums myself and I speak with clients. So far, the only negative comment I got was its cost. Very effective but pricey. I understand you want to sell but I am also trying to tell people that this is what we do so they can have options. For additional info, we do prp for severe dry eyes too and we use prp tubes for more growth factors. I don’t really share confidential infos.
@@PRPLabs BD is FDA approved too. BD do not claim that they are endotoxins free. It’s like saying they are not peanut free facility for precaution. The only difference of the bd tubes to PRP tubes is the number of growth factors.
@@SeaBee1223 BD Vacutainers are class 1 medical devices which are FDA cleared for general chemistry purposes only. These are very different from class 2 medical devices that are specifically FDA cleared for preparing autologous PRP. Saying BD is "FDA approved" is not only inaccurate terminology (since they are FDA "cleared" not "approved") - it's also irrelevant because their FDA clearance has nothing to do with the preparation of PRP. The manufacturer's of FDA Cleared PRP devices will all proudly acknowledge that their products are endotoxin free. While some people are allergic to peanuts and may avoid food manufactured around peanuts, ALL patients want to avoid endotoxins being put in their eyes. So it's probably best that you and your team do everything you can to avoid that, even if that means using appropriate medical devices for your procedures.
@@SeaBee1223 If your surgeons don't know that BD tubes are not endotoxin free, then they have not had enough education. Are they using PRP tubes that are endotoxin free? Or, are they using PRP tubes that contain endotoxins and they are injecting endotoxins into patients? As for patients only complaining about the cost: Do you really think patients would know when endotoxins are being injected into their body and their body is reacting at a microscopic level? You've said you're using BD tubes. BD states that they are not endotoxin free. Therefore your surgeons are injecting endotoxins into patients because they are using BD tubes.
The 5th question on the BD.com FAQ states that all their blood collection tubes may contain endotoxins: www.bd.com/en-ca/offerings/capabilities/specimen-collection/blood-specimen-collection/venous-collection/bd-vacutainer-blood-collection-tubes/vacutainer-blood-collection-tube-faq/general-tubes-faq This is not a benefit, but rather a disadvantage that comes with less expensive manufacturing processes.
Why tell people what not to use but you never said what should be used! This sounds like a buy mine only type of sales pitch!
Please feel free to buy any PRP kit that is registered with the FDA :)
Another doctor on TH-cam said acda is citric acid and dextrose, non toxic
@@KrisD007 ACD-A is a commonly used anticoagulant and is generally considered safe. However, the issue with vacutainers is not this ingredient. The issue is that vacutainers are not FDA cleared to create PRP and that they may contain endotoxins - according to the manufacturer of Vacutainers, BD: "BD does not claim that any of the Vacutainer blood collection tubes are endotoxin free."
www.bd.com/en-ca/offerings/capabilities/specimen-collection/blood-specimen-collection/venous-collection/bd-vacutainer-blood-collection-tubes/vacutainer-blood-collection-tube-faq/general-tubes-faq#:~:text=(10%20boxes).-,Q.,collection%20tubes%20are%20endotoxin%20free.
4 real!!!! Extremely annoying
It is. This lab sucks.
The glass BD ACD-A Vacutainers (the ones he's holding) have been proven to not contain endotoxins. They have been repeatedly tested and verified in several published studies to be not only endotoxin free, but are ideal for even intra-articular PRP injections in which absolute sterility has to be maintained.
This 2004 study found endotoxins in glass BD Vacutainers: academic.oup.com/clinchem/article/50/11/2215/5640121
It's also worth noting that your confidence of BD ACD-A Vacutainers being endotoxin free is not shared by the manufacturer of these tubes. To quote BD's own website:
"BD does not claim that any of the Vacutainer blood collection tubes are endotoxin free."
www.bd.com/en-ca/offerings/capabilities/specimen-collection/blood-specimen-collection/venous-collection/bd-vacutainer-blood-collection-tubes/vacutainer-blood-collection-tube-faq/general-tubes-faq#:~:text=(10%20boxes).-,Q.,collection%20tubes%20are%20endotoxin%20free.
why would they make a claim that has no bearing on the designed purpose. why would they contain endotoxins? logically why would they? @@PRPLabs
I'm not sure where there's any confusion - you can read the study we posted above your comment. A published, peer-reviewed study has demonstrated the BD Vacutainers were found to contain endotoxins. It's not a hypothetical matter - it's a documented fact.@@bballev2
'The 5-mL heparin Vacutainer Tubes (lot no. 3224585) were positive for endotoxin (10 ng/L), whereas another lot of 5-mL tubes (lot no. 4029101), 4-mL plastic heparin tubes, and 10-mL glass heparin tubes were negative for endotoxin (
but dont worry, im linking this video and your site to the manufacturer. im sure youll be getting a more in depth feedback from them. @@PRPLabs
sir plz tell centrifuge @ rate ,or how much time we have to centrifuge at what rpm single or double spin ? and how much sample of prp in ml is needed for 1 sitting ?
Usually 3000 RPM for 10 minutes
What tube should be used for blood collection? Plasma comes from unclothed blood so the tube must have some additives to prevent clotting.
Not if you want PRF….
Ok so what is exactly in yours?
we are in hong kong - do you have supplier here so we can order ?
We only sell to US-based medical providers.
Do you have an alternative to suggest?
There are a variety of FDA cleared PRP Kits on the market. You can see the ones we offer on our website www.prplabs.com/prp-kits
@@PRPLabs I missed that this isn't for consumers.
Is it illegal to inject PRP made with vacutainer (or any other vessel which is not FDA cleared as PRP kit) to patient in the US?
Or is it just literally ‘not good for health’?
It is against FDA regulations (and therefore illegal) to inject PRP into a patient that was prepared in a device that has not been FDA cleared for PRP preparation. Additionally, it is not good for health to inject endotoxins (which are found in vacutainers) into a patient's body.
what do you expect they answer? they sell other equipement
@@andybaubau5961they were asking for their own use. It’s not illegal to do this at home for your own use.
stop misleading people. not claiming to be endotoxin free is not the same as saying that they contain endotoxins. youre a dip@@PRPLabs
@@PRPLabs can you show me where in the FDA guidance it says this please. (for my own knowledge)
Very informative! Just a question: FDA approval is required only in the United States, correct? But I don't live in the US and I'm interested to see a dermatologist for PRP therapy for hair regrowth. I'm just watching a bunch of related videos to know more about the procedure before take that decision. But I'm a little afraid right now. How can I make sure that they will not inject those endotoxins on my scalp?
The FDA is a US-based regulatory agency and it's jurisdiction would not extend to countries outside of US jurisdiction. We can't advise on how to find appropriate medical devices outside of the US.
The way to make sure that endotoxins are not injected into your scalp is to call your dermatologist and ask what brands and models of PRP tubes they use. With some patience and encouragement they should provide you with that information. Once you have the brands and models that they use, research that info to find out if the tubes contain endotoxins or not. If they don't, make your appointment for the procedure. At the appointment, right before they are about to start the procedure, verify for yourself that they are indeed using the PRP tubes they said that they use that are endotoxin free.
Do you send to individuals? I can do self hair prp.
We only sell to licensed US-based medical providers.
Is BD ACD A PLUS BIOTIN GEL vial IS BETTER THAN Plain ACD A vial? plz help
BD Vacutainers should not be used for PRP. Both of the options that you listed are not suitable.
Hello i have been asking loads but no answer good evening sir hope you are doing well. What sort of Blood Collection Tubes
Can i put blood in and drink it from don't need a lot but i need to try this so i want to do it in a save way but no one will let me know im getting left to do this in a unsafe way please help many thanks take care
The word endotoxin is very scary, its essentially parts of cell walls of Gram negative bacteria that is often released into the surrounding environment. In infected tissues these endotoxin concentrations are extremely high and cause significant inflammation, might it be that endotoxins are left over in the tubes because they've been sterilized properly. Now if some endotoxin which is an proinflammatory substance not a bacteria, is left over enough to be detectable in the BD tubes then that makes sense since the tube was sterilized but does that mean it causes illness/disease, pretty unlikely considering the concentration of endotoxin required would need to be very high. Considering that in PRP not all the fluid is remove from the tubes for injection, only the platelet rich plasma which is less than 1/5th the total blood volume drawn, further diluting the endotoxins exposure. This is a scare tactic people, endotoxins can't cause problems unless in very high concentration as in the conditions that only infection can cause, if these tubes were not sterilized, which they claim to be then significant infections would result.
The one study used as "fact" showed endotoxins present one Lot of BD vacutainers that had Sodium heparin additive which are not used for PRP, ACD-A is, are there any in ACD-A Yellow Top vacutainers probably but the only results would be inflammation which PRP treats!
- Critical thinking suggests this video is hyperbole to sell a product. Yes, I'm a medical professional.
BD says on their website "BD does not claim that any of the Vacutainer blood collection tubes are endotoxin free." So it's their company admitting they have endotoxins in their tubes - not just a study.
We suspect that most patients would prefer to receive PRP from medical devices that are FDA cleared for the preparation of PRP and that do not contain endotoxins.
However, if as a medical provider, you feel comfortable using non-FDA cleared vacutainers, which contain endotoxins, to make PRP - perhaps you should mention your clinic name so patients know where to seek your discount services.
Most patients would prefer to receive PRP from medical professionals who use FDA-cleared medical devices that do not contain endotoxins an that are intended for making PRP. But maybe there are some thrill seekers who prefer providers like you who like to cut corners and do it the cheap way - even if it's not safe or federally compliant. Would you like to share your practice name in the comments so everyone can know where to find your unconventional services?
Also, BD says on the FAQ on their website that all of their blood collection tubes can contain endotoxins. This is shown in the video and you can look it up on their website today. I'm not sure why you're trying to discredit this fact that is plainly available for all to see.
Can a home user purchase from you, or do you have to own a clinic?
The legit ones usually ask for proof that you are a healthcare worker and company you work for.
I stole vactubes from blood bank and now you say this?
Lol
I use this tube for prp once made me burn the skin was burning
Nah, of course they have to sell. I work with surgeons and we use BD tubes for PPP eye serums. Are you saying we are putting endotoxins in our patients’ eyes? Lol. We use prp tubes from a different company for other eye and skin treatments too. So far, no complains of endotoxins from our patients cos our team of doctors could have known that, right?
Please share the name of the hospital you work for so we can help inform your patients that YES - you are likely putting endotoxins into your patients' eyes.
BD says on their own website that all of their blood collection tubes contain endotoxins. Read for yourself on the 5th question on this FAQ: www.bd.com/en-ca/offerings/capabilities/specimen-collection/blood-specimen-collection/venous-collection/bd-vacutainer-blood-collection-tubes/vacutainer-blood-collection-tube-faq/general-tubes-faq
"Q. Are BD Vacutainer blood collection tubes endotoxin free?
A. No, BD does not claim that any of the Vacutainer blood collection tubes are endotoxin free."
Sadly a lot of doctors do not understand the risks of illegally using blood collection tubes to make PRP. That's why we release educational content like this video. If the doctors at your clinic want to make PRP that is safe and effective for your patients, then they should use a medical device that is FDA cleared for the preparation of autologous PRP.
Seriously, surgeons have had enough education. They are very specific and careful when it comes to what they put in their patients’ eyes. I make the eye serums myself and I speak with clients. So far, the only negative comment I got was its cost. Very effective but pricey. I understand you want to sell but I am also trying to tell people that this is what we do so they can have options. For additional info, we do prp for severe dry eyes too and we use prp tubes for more growth factors. I don’t really share confidential infos.
@@PRPLabs BD is FDA approved too. BD do not claim that they are endotoxins free. It’s like saying they are not peanut free facility for precaution. The only difference of the bd tubes to PRP tubes is the number of growth factors.
@@SeaBee1223 BD Vacutainers are class 1 medical devices which are FDA cleared for general chemistry purposes only. These are very different from class 2 medical devices that are specifically FDA cleared for preparing autologous PRP.
Saying BD is "FDA approved" is not only inaccurate terminology (since they are FDA "cleared" not "approved") - it's also irrelevant because their FDA clearance has nothing to do with the preparation of PRP.
The manufacturer's of FDA Cleared PRP devices will all proudly acknowledge that their products are endotoxin free.
While some people are allergic to peanuts and may avoid food manufactured around peanuts, ALL patients want to avoid endotoxins being put in their eyes. So it's probably best that you and your team do everything you can to avoid that, even if that means using appropriate medical devices for your procedures.
@@SeaBee1223 If your surgeons don't know that BD tubes are not endotoxin free, then they have not had enough education. Are they using PRP tubes that are endotoxin free? Or, are they using PRP tubes that contain endotoxins and they are injecting endotoxins into patients? As for patients only complaining about the cost: Do you really think patients would know when endotoxins are being injected into their body and their body is reacting at a microscopic level? You've said you're using BD tubes. BD states that they are not endotoxin free. Therefore your surgeons are injecting endotoxins into patients because they are using BD tubes.
What do you recommend using them for prp???
We recommend using an FDA cleared PRP system
Can we use Edta or sodium citrate tube for PRP??
We would not recommend that.
@@PRPLabsgreat marketing aspect to promote your business 👏🏻
thank you for making this video
How is Acid Citric Dextrose toxic?
We never said it was.
They don’t explain cos they don’t really know. They can only provide you safe answers for their company
I can’t seem to find why sst tubes (hold top) even have endotoxins in them? What benefit does it have ?
The 5th question on the BD.com FAQ states that all their blood collection tubes may contain endotoxins:
www.bd.com/en-ca/offerings/capabilities/specimen-collection/blood-specimen-collection/venous-collection/bd-vacutainer-blood-collection-tubes/vacutainer-blood-collection-tube-faq/general-tubes-faq
This is not a benefit, but rather a disadvantage that comes with less expensive manufacturing processes.
Thank You 😊
So what used
In the USA, our hope is that all medical professionals offering PRP therapy are using equipment that is FDA cleared for creating PRP
@@PRPLabs ACD A is FDA approved no?
@@servantofthecreator they are still looking for answers. They can’t really explain it
Are endotoxins the same as endo-smoke?😂