Fluorescence and Compensation in Flow Cytometry

For more information, bio-rad.com/flowcytometry
Fluorophores accept light at a specific wavelength and re-emit it at a higher wavelength, giving each fluorophore an excitation and emission wavelength. Although there is a peak or maximal emission, the range is often several hundred nanometers. This can cause problems where unwanted light enters several detectors giving false positives. Compensation is required to remove this unwanted fluorescence. Careful choice of fluorophores can reduce the amount of compensation needed.
This webinar will cover:
- Basic fluorescence principles
- What is compensation and why you need to apply it
- Compensation controls
- How to avoid compensation in small panels
- Common rules to help build more complex panels
This webinar is recommended for novices and researchers with limited experience of flow cytometry.
Visit us on the web to learn more:
www.bio-rad.com/en-us/category...

[@eziac78]

Go to 2:35 if you want to skip the intro

[@user-vm7xe2hs5v]

Is it recommended to do your single stains with cells for Compensation or use compensation beads instead?