Video 10: Western blot

[@melaniesoria767]

🎯 Key points for quick navigation:
00:08 *Metabolic diseases lab*
00:38 *Western blot technique*
01:03 *Protein molecular weight*
01:17 *Antibody-protein interaction*
01:54 *Secondary antibody binding*
02:08 *Fluorescent detection dyes*
02:23 *Antibody host species*
03:07 *Gel membrane transfer*
03:50 *Methanol membrane soaking*
04:21 *Filter pad placement*
05:15 *Air bubble removal*
06:01 *Blocking solution preparation*
07:03 *Non-specific binding prevention*
08:14 *Primary antibody incubation*
09:20 *Membrane washing optimization*
10:01 *Secondary antibody selection*
10:41 *Chemiluminescence reaction process*
11:39 *Protein size marking*
12:46 *Membrane reuse preparation*
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[@garymijailramosreyes7093]

Excelente :)