I really appreciate your selfless service,your way of explanation is concise and easy to grasp especially for virtual learner like me..😇 Keep it up..!! Thank you Hussain Biology 👍...!!!💕💕🙏
@@hussainbiology 3:45 You wrote that Flap is to be removed from 5' end but You removed a segment of dna(Flap) from 3' end of the newly synthesizing okazaki fragment!????? 🤔
Does this RNase H remove primers in both the leading and lagging strands? There were mentions that RNase H works on the leading strand while the Flap model works on the lagging strand only.
Hello sir. Very detailed lecture. May i ask you which books you refer to for Molecular genetics, comprising of this Replication, Transcription and Translation topics. A reply would be helpful. Thanks for the video.
Dna polymerase delta replicates only leading strand as you mentioned then lagging strand is supposes to be replicated by Dna polymerase epsilon, please provide the insight, kinda confuse at that portion. Thank you for your efforts.
Plz Refer to this also : www.cell.com/molecular-cell/fulltext/S1097-2765(18)30879-7?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1097276518308797%3Fshowall%3Dtrue
All the above articles explains the dual activity of Delta Polymerase in leading and lagging strand synthesis. But there is still ambiguity in the enzymes of Dna Replication
Love your videos! The synthesis in the lagging strand works the same in prokaryotes? I dont remember watching FEN1 in those videos... With prokaryotes is only the ligase and RNAse is a simpler process?
DNA ligase forms two covalent phosphodiester bonds between 3' hydroxyl ends of one nucleotide ("acceptor"), with the 5' phosphate end of another ("donor")
How come there’s only a leading strand on the top and not on the bottom as well? Shouldn’t the top of the replicon also have its own Okazaki fragments? Or is this different from prokaryotic replication?
Sir , I find a little fault in the part that primer gaps are not filled by pol alpha rather its filled by continuous elongation of pol delta . Please tell me and clear my doubt.
@@hussainbiologybro after searching a lot on google i finally find that #the RNA primer removal is carried out by enzymes RNase H and FEN 1. This gap created by RNA removal is filled by continued elongation of the new okazaki fragment ( carried out by polymerase delta) pol . Delta elongates the okazaki fragment to the final length of about 150_200 bp.The small nick that remains is finally sealed by DNA ligase... & Plz tell me is this correct or not ...
Yes that is true.....The PRIM1 AND PRIM2 units of Dna Polymerase Alpha acts as a DNA primase enzyme...... That is why i have shown it separately for understanding and furthermore we have ccdc111 molecule (AEP family) which is both a primase and polymerase enzyme ( mostly used in Translesioñ synthesis). Still thanks for adding this part of information.
Ur videos are best but u are lecturing so fast ..I am upset about that. . I can't grasp everything so quickly. Don't know about others. So plz make videos a bit slower in speed
Having confusion in why in lagging strand different primers are needed... Although the replication is going towards away from the replication fork... But Dna polymerase only needs 3 hydroxyl end if one primer provides the 3 hydroxyl end then why different primers are needed....???? ... Plz pin the answer sir😟😟
Actually when the Replication Bubble opens , it only opens a small segment, so when we put on first primer , the Polymerase comes and starts synthesizing on 3 prime end... Then again Dna Strand opens and that time we are devoid of any 3 OH end.. so we again need primer
no the leading strand is 3'-5', because dna polymerase synthesizes the dna in the 5'-3' anti parallel direction, thats why the lagging strand is 5'-3', because dna polymerase CANNOT synthesize dna from 3'-5' continuously (okazaki fragments)
Asser is right, u need to understand the way how the nucleotides get attached onto each other. All know Poly. connect dNTPs on the free 3' OH Group(of the new synthesized string) with the 5' phosphat end of the new nucleoside. You may find it easy to remember that if you know that the needed energy for this connection gets from the reduction of the dN->TRI phosphatMONO phostphat
Short brief and to the point video... Sir you don't know how much your lectures has helped me. Thank you so very much..!
I really appreciate your selfless service,your way of explanation is concise and easy to grasp especially for virtual learner like me..😇
Keep it up..!! Thank you Hussain Biology 👍...!!!💕💕🙏
thanks for appreciation... Really overwhelmed by ur support
@@hussainbiology 3:45 You wrote that Flap is to be removed from 5' end but You removed a segment of dna(Flap) from 3' end of the newly synthesizing okazaki fragment!????? 🤔
Respect from Iran....perfect presentation..thanks a lot Sir...
thanks Yousef for appreciation....Glad to know that it helps
U r really amazing sir...right now ur videos are equal to my number of teacher's.....🙏🙏🙏....stay blessed sir
thanks for appreciation..Glad to know that it helps..Keep sharing and supporting ✌️
You are boon to people like us....thnku somuch🥺hoep will clear my internals writing all these.
thanks for appreciation...Glad to know that it helps
Thxs for this lecture 🙏🙏struggling with this topic from so many days finally understood .you are really an amazing teacher🔥🔥
thank you soo much you are a gift to the med students world wide
Good Job! Helped me understanding the role of FEN1 so far...
thanks for appreciation...Glad to know that it helps.
You explain to the point and beautiful diagram. Only issue in some of your pronunciation. thank you so much.
thanks for appreciation....Glad to know that it helps..... and apologies for the weird pronunciation
what a perfect lecture. I really enjoy persentation...
thanks for appreciation
Just amazing how you explain the thing ,just Fabulous
thanks Laila for appreciation....Glad it helps ✌️
No words!! just awesommmmeeee!!!!
❤️ thanks for appreciation
Thank you sir. Stay blessed 😇
Most welcome.... Allah bless u too
thank you soo much for doing such a nobel job
thanks Neera for appreciation....Glad to know that it helps
Congratulations Dear for 20k
Shukriya boi....
Your videos are so helpful! Thank you so much!
Really helpfull thanks a lot sir
thanks for appreciation...Glad to know that it helps ✌️
I read the leading strand is polymerised by DNA polymerase EPSILON. Correct me if I'm wrong. Btw, brilliant explanation!! Thank you.
This video is referenced to the recent studies.
www.ncbi.nlm.nih.gov/pmc/articles/PMC4517859/
New study reveals that Polymerase Delta synthesizes both strands.
@@hussainbiology thank you.
Does this RNase H remove primers in both the leading and lagging strands? There were mentions that RNase H works on the leading strand while the Flap model works on the lagging strand only.
Nice presenting but they are two different DNA polymerisation enzymes that synthesis leading and lagging strand
U are not explained about looping that occurs in lagging stand
Best video 👍
This was so helpful thank you I appreciate your work✌️💓
thanks for appreciation..Glad to know that it helps ✌️
Hello sir. Very detailed lecture. May i ask you which books you refer to for Molecular genetics, comprising of this Replication, Transcription and Translation topics. A reply would be helpful.
Thanks for the video.
Watson is a great book for molecular biology.
And now moving on to your termination video!
Dna polymerase delta replicates only leading strand as you mentioned then lagging strand is supposes to be replicated by Dna polymerase epsilon, please provide the insight, kinda confuse at that portion. Thank you for your efforts.
This Recent Article explains the Role of Pol Delta :
www.ncbi.nlm.nih.gov/pmc/articles/PMC4517859/
Plz Refer to this also :
www.cell.com/molecular-cell/fulltext/S1097-2765(18)30879-7?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1097276518308797%3Fshowall%3Dtrue
All the above articles explains the dual activity of Delta Polymerase in leading and lagging strand synthesis.
But there is still ambiguity in the enzymes of Dna Replication
@@hussainbiology Thank you so much!
@@anamikagarg9703 You are welcome
Love your videos!
The synthesis in the lagging strand works the same in prokaryotes? I dont remember watching FEN1 in those videos...
With prokaryotes is only the ligase and RNAse is a simpler process?
Leading & lagging strand are present on both the sides.
On which end (3' or 5')DNA ligase attach first to join okazaki fragements
DNA ligase forms two covalent phosphodiester bonds between 3' hydroxyl ends of one nucleotide ("acceptor"), with the 5' phosphate end of another ("donor")
Thnku sir
How come there’s only a leading strand on the top and not on the bottom as well? Shouldn’t the top of the replicon also have its own Okazaki fragments? Or is this different from prokaryotic replication?
My teacher told me that leading and legging strand are present on both up and down side is that true?
It's not about up and down
the strand syn. Depends on orientation
5 to 3 prime is down then leading strand will be down vice versa
Sir , I find a little fault in the part that primer gaps are not filled by pol alpha rather its filled by continuous elongation of pol delta . Please tell me and clear my doubt.
Hey bro gaps left by okazaki fragments are filled with which enzyme... Plz explain i m not understanding it..
Polymerase Alpha fills the gaps and DNA ligase ligates the fragments....
@@hussainbiologybro after searching a lot on google i finally find that #the RNA primer removal is carried out by enzymes RNase H and FEN 1. This gap created by RNA removal is filled by continued elongation of the new okazaki fragment ( carried out by polymerase delta) pol . Delta elongates the okazaki fragment to the final length of about 150_200 bp.The small nick that remains is finally sealed by DNA ligase... & Plz tell me is this correct or not ...
Sir please clear alpha synthesises primer gaps or delta at the end?
Poly alpha Or poly delta fill the gaps??
In Cooper it's poly delta
I m confused
good jobe but ther is no primase in eucaryote cell ... its the activity (the synthese of primere) of polymerase alpha primase chez les eucaryote
Yes that is true.....The PRIM1 AND PRIM2 units of Dna Polymerase Alpha acts as a DNA primase enzyme......
That is why i have shown it separately for understanding and furthermore we have ccdc111 molecule (AEP family) which is both a primase and polymerase enzyme ( mostly used in Translesioñ synthesis).
Still thanks for adding this part of information.
Hussain Biology thank u
I have a question about the toposomarases....where is 1 used and I thought 2 was used for termination not removing supercoilization.
that's telomerase
Thank you Hussain ❤️
Thanks Nasrin for appreciation...Glad to know that it helps.
Which book do you refer?? And how do you understand so easily??
I use Molecular Cell Bio by Alberts and Molecular Cell Bio by Lodish plus i also takes recent information from NCBI and Sciencedirect sites.
and I have to understand it anyway bcz this is why i am here to put complex mechanisms into easy way. Thanks Sheeba for appreciation....
Thank you so much 😪I have got my mol bio exam and I'm referring your videos thank you so much
@@sheebazafer5476 Glad to know that. All the best for exams.
And I'm gonna forward all your videos to my classmates
Ur videos are best but u are lecturing so fast ..I am upset about that. . I can't grasp everything so quickly. Don't know about others. So plz make videos a bit slower in speed
Hey Dr N... First of all apologies for that...
and i will definitely slow down in new videos .. thanks
Termination process?? 😢
👍👍
Seen a video having 150 likes & 0 dislikes.
thanks buddy.....keep sharing and supporting
Having confusion in why in lagging strand different primers are needed... Although the replication is going towards away from the replication fork... But Dna polymerase only needs 3 hydroxyl end if one primer provides the 3 hydroxyl end then why different primers are needed....???? ... Plz pin the answer sir😟😟
Actually when the Replication Bubble opens , it only opens a small segment, so when we put on first primer , the Polymerase comes and starts synthesizing on 3 prime end...
Then again Dna Strand opens and that time we are devoid of any 3 OH end.. so we again need primer
@@hussainbiology ok sir thank you... But plz sir if u are having a time plz upload a short vedio for it.....
@@misbashabir6514 for what.. Like alag sa video for that lagging strand confusion..
@@hussainbiology yes sir
@@misbashabir6514 I will send u , image or short video showing how it is done
Thanks you
thanks for appreciation..Glad to know that it helps
the leading strand run from 5' ----- 3' direction, u made opposite
no the leading strand is 3'-5', because dna polymerase synthesizes the dna in the 5'-3' anti parallel direction, thats why the lagging strand is 5'-3', because dna polymerase CANNOT synthesize dna from 3'-5' continuously (okazaki fragments)
Asser is right, u need to understand the way how the nucleotides get attached onto each other. All know Poly. connect dNTPs on the free 3' OH Group(of the new synthesized string) with the 5' phosphat end of the new nucleoside. You may find it easy to remember that if you know that the needed energy for this connection gets from the reduction of the dN->TRI phosphatMONO phostphat
Reference??
Zenab , it is from :
✓Lodish Cell Biology
✓Alberts Cell Biology
✓NCBI
✓Science Direct
✓ Frontiers
✌️✌️
👍👍👍👍
😊😊😊😊
Please make vd in hindi
Is very hard understand ur pronunciation for a no native english speakear, and the subtitles doen´t correspond
apologies
👍👍