20191016-CPGR-FragPipe-MSFragger-IDPicker
ฝัง
- เผยแพร่เมื่อ 22 ส.ค. 2024
- As part of the CPGR's hands-on tutorial in proteomics, I trained the students on the use of database search engines to identify proteins for comparative proteomics experiments. In this video I demonstrate the use of the FragPipe GUI to launch MSFragger in a typical "narrow" search. The pepXMLs that we created could then be interpreted through IDPicker to produce a spectral count table. Through MSFragger did not require us to change data format from RAW to mzML or MGF, I also showed the use of ProteoWizard msConvertGUI. The data we employed came from the ABRF iPRG 2009 "red" vs. "yellow" E. coli challenge.
Very useful. Thank you very much.
Thank you for this excellent tutorial
a great lecture, thank you!