Bulk RNA-Seq Analysis Demystified: Step-by-Step Tutorial Part 6 Read Alignment or Mapping by HISAT2

Welcome to Part 6 of our Bulk RNA-Seq Analysis series! In this tutorial, we'll dive into the critical steps of read alignment or mapping using HISAT2. By the end of this video, you will learn how to:
🔹 Download a reference genome
🔹 Index the reference genome using HISAT2
🔹 Perform read alignment with HISAT2
🔹 Convert SAM to BAM files using SAMtools
🔹 Sort BAM files using SAMtools
These steps are essential for accurate RNA-Seq data analysis, ensuring your data is ready for downstream processing and interpretation.
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#RNASeq #HISAT2 #bioinformatics #samtools #dataanalysis
Disclaimer:
The information provided in this tutorial is intended for educational and instructional purposes only. The tutorial creator and platform do not assume any liability for any errors, omissions, or inaccuracies in the content. It is essential to exercise caution and follow best practices when conducting experiments and data analysis in a laboratory setting.