Basics of lab centrifuges - rpm vs. rcf, swinging bucket vs. fixed angle, ultra vs. "normal", etc.

[@kevintotimeh6648]

Excellent video as always, it makes sense why Newtonian forces are applied to the centrifuges' rate of motion. Also, interesting units, I never heard of rcf.

[@thebumblingbiochemist]

Thanks!

[@funny11744]

Great work.Many explanation, a real teacher.

[@thebumblingbiochemist]

Thanks so much!

[@funny11744]

I have a 10 ml centrifuge with fix angle and a minimum radius and a maximum radius . I do not find the radius in the centrifuge manual but it is written RCF =1150 g for 3200 rot /min. What would be the (average) radius for calculation of RCF ? Is it 10 cm ? Could the radius be artificial increased by decreasing the volume in the tube from 10 ml to 3 ml for example ?

[@thebumblingbiochemist]

I don't know sorry but changing the volume won't change the radius

[@funny11744]

@@thebumblingbiochemist IT depends on the radius .If it is the maximum radius - the RCF does not change but If it is about an average radius ( for fixed angle centrifuge only) between the minimum and maximum, then RCF is changing once with decreasing the minimum radius ( meaning decreasing of the level of the liquid in the tube).

[@thebumblingbiochemist]

Gotcha. Sorry I misunderstood your question

[@funny11744]

Could the Amicon 100 be used with fixed angle centrifuge ?

[@thebumblingbiochemist]

Yes - but you may need to adjust the speed and volume - the product manual will have more information.

[@funny11744]

@@thebumblingbiochemist thank you.

[@merajj]

great work as always

[@CheMisTcLuB]

thanks a lot, you have no idea how much you help with this explanation

[@thebumblingbiochemist]

Glad to hear that!

[@IlmiMulhid]

Thanks 🙏