and thank you so much for your demonstration! I got a question that for one Flex Set like mIL-2, do we just need to do one-time standard curve after setup? and the following sample tests just based off that standard curve?
You're welcome! For a Flex Set like mIL-2, you typically need to create a standard curve each time you run a new set of samples. This ensures accuracy and accounts for any variations in experimental conditions. The standard curve generated during the initial setup can guide you, but it’s essential to run a fresh standard curve with each new batch of sample tests to ensure reliable and consistent results. Thank you!
as per the manual, we only used 500 ul as top standard and 500 ul for the following dilution, and no store or reuse reconstitute standards as suggested.
Hi! Yes, it is generally recommended to discard the remaining 3 ml of the reconstituted standard solution. Reconstituted standards can degrade over time and may not provide accurate results if reused. Always prepare fresh standards for each assay to ensure the reliability and accuracy of your results. Thank you!
I have already uploaded the video for CBA kit analysis by flowjo Link is here th-cam.com/video/JojmR-BC5lU/w-d-xo.html And also will try to make video on cba flex set data analysis as soon as possible
Hi Shashi Bhushan, I won't have all my samples on the same day, any recommendations for keeping them? They are plasma samples. Thank you so much for the video.
Great video sir
Thanks Rahul
I'm about to run this assay and your video was really helpful! Thank you
and thank you so much for your demonstration! I got a question that for one Flex Set like mIL-2, do we just need to do one-time standard curve after setup? and the following sample tests just based off that standard curve?
You're welcome! For a Flex Set like mIL-2, you typically need to create a standard curve each time you run a new set of samples. This ensures accuracy and accounts for any variations in experimental conditions. The standard curve generated during the initial setup can guide you, but it’s essential to run a fresh standard curve with each new batch of sample tests to ensure reliable and consistent results. Thank you!
Thank you so much for this video. It's helped me a lot!
Thank you
Hiiii, should we just discard the 3 ml left reconstitute standard solution?
as per the manual, we only used 500 ul as top standard and 500 ul for the following dilution, and no store or reuse reconstitute standards as suggested.
Hi! Yes, it is generally recommended to discard the remaining 3 ml of the reconstituted standard solution. Reconstituted standards can degrade over time and may not provide accurate results if reused. Always prepare fresh standards for each assay to ensure the reliability and accuracy of your results. Thank you!
thanks so much , could please make a video on cytometer setup procedure using cytometer set up beads too? both for kit and flex set one.
I have already uploaded the video for CBA kit analysis by flowjo
Link is here th-cam.com/video/JojmR-BC5lU/w-d-xo.html
And also will try to make video on cba flex set data analysis as soon as possible
For Cytometer setup, I am working on it and will upload the video soon
Can you please tell if this acquisition could be taken at BD Accuri?
Do we have to neccessarily have FCAP software for this type of assay?
Hi Shashi Bhushan, I won't have all my samples on the same day, any recommendations for keeping them? They are plasma samples. Thank you so much for the video.
Explanation of what is CBA would made it more interesting, instead of directly starting the experiment.