very good post, i have a correction here, at 14:10 you said that you can separate RNA and DNA with size exclusion chromatography which is not true RNA and DNA can be separated by gel electrophoresis :)
Before commenting you should know what you are telling. By the way in GC, separation is not based on boiling point. It is based on affinity of samples towards stationary phase and mobile phase( career Gas)
In isocratic separations, analyte retention time can be normalised to retention factor, k, which allows a direct comparison between columns with different dimensions. This is possible due to the constant mobile phase composition used in isocratic methods.Gradient retention factor, k*, also known as average k, can be used to describe the chromatographic conditions. k* requires a range of 2 < k* < 10, similar to that of retention factor, k. If k* is below 2, there is potentially insufficient interaction between the analyte and the stationary phase, whilst above 10, the run times can be excessive, which can decrease laboratory productivity and increase solvent consumption
Column back pressure is back pressure due to Column. As mobile phase passes through Column containing stationary phase of fine particle (3 micron or 5 micron), the pressure is generated.
Very nice video lec..more important and helpfull..thankyou 🎉
Excellent lecture, thank you to all team members.....
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Very very excellent Dr. Sonal. Thanks for such a useful piece of information.
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Excellent very easy to understand and who prepared for newly in interview purpose will deffinately help ... Thank u for this vedio. .
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Excellent & highly useful presentation on HPLC, Thank you mam.
Thank u so much and ur explaining style is beautiful & easy to learn!
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Thank mam for presenting such knowledge for chromatography..
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Tq lot of learning ponits for hplc
Ur video
Excellent presentation of different aspects of HPLC, Thank you mam.
Thank you
Good job
i am a teacher as well as lab instrument trainer
i appreciate yu
your convying mthd z very good
Size Exclusion chromatography is also called as Gel Permeation Chromatography or Gel Filtration Chromatography based on Stationary phase.
Thanks a lot mam for the detailed presentation in such a lucid way.
Sanket want to talk same things
Very nice lecture on HPLC. Thank you.
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Thanks for sharing valuable information on different aspects of HPLC
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Nice lecture on different aspects of HPLC method development.
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You are doing great ❤
Excellent explanation thanks 🙏
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Thanks mam
You lecture was very useful for me
Very well explained...thank you ma'am 👍
Excellent lecture.
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Thank you very much for your efforts and do you have any information about validation HPLC?
Excellent💯💯💯
Very good explanation on method Development of HPLC
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Very thankful madam 💓💓💓💓
Thanks for nice explanation mam its really helpful
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Helpful information 👍🏻👍🏻👍🏻👍🏻
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Thanks mam
It was very fruitful for me
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Nice video. Keep it up👍🏻
Excellent madam.. Very nicely explained..
Thanks a lot
Outstanding presentation 👏
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Fantastic
very good post, i have a correction here, at 14:10 you said that you can separate RNA and DNA with size exclusion chromatography which is not true RNA and DNA can be separated by gel electrophoresis :)
Also check article Rapid purification of RNAs using fast performance liquid chromatography (FPLC)
Insil Kim,1 Sean A. McKenna,1
2007
Size Exclusion chromatography is also called as Gel Permeation Chromatography or Gel Filtration Chromatography based on Stationary phase.
Very good information and expline medam
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Very nice knowledge full points👍
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Excellent explanation of HPLC
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Thank you
36 minutes chiral detector ion detector comes under which property detector bulk or solute
excellent lecturing madam
Explanation is best.
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good information...
Madam please du more videos regarding method development... What are the common problems we are facing while performing method development...
Totally Good 😃😊
Before commenting you should know what you are telling. By the way in GC, separation is not based on boiling point. It is based on affinity of samples towards stationary phase and mobile phase( career Gas)
Excellent PPT and excellent explanation
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Nicely explained and very useful beginners
Thank you. Share and subscribe. Also watch th-cam.com/video/7wAWve4Ekqk/w-d-xo.html
Very very excellent presentation thanks mam
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Good lecture
excellent presentation
Many thanks
nice session on HPLC method development
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Thank you so much mam
Excellent lecture
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Excellent lecture madam,,,, thanks🙏..........
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Excellent
Thanks
Excellent lecture mam
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th-cam.com/video/aMxwDkKm3is/w-d-xo.html
Superb mam, 🙏
Thank you madam... good explanation...
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What is the use of retention factor and gradient retention factor
In isocratic separations, analyte retention time can be normalised to retention factor, k, which allows a direct comparison between columns with different dimensions. This is possible due to the constant mobile phase composition used in isocratic methods.Gradient retention factor, k*, also known as average k, can be used to describe the chromatographic conditions. k* requires a range of 2 < k* < 10, similar to that of retention factor, k. If k* is below 2, there is potentially insufficient interaction between the analyte and the stationary phase, whilst above 10, the run times can be excessive, which can decrease laboratory productivity and increase solvent consumption
Nice video
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Very excellent
Thank you. Share with others
th-cam.com/video/aMxwDkKm3is/w-d-xo.html
Salute to mam💐💐
How do we know that from structure our compound is ionizabale and non ionizabale
Generally salts of acids and bases are ionizable in nature
@@analyticaltechniques7088 thank you
Thanks ma'am
Most welcome 😊
Your explaining very nice madam
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Excellent mam.
Can you explain GC
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Interesting lecture
Many thanks!
Column back pressure increases in short or long column17.48 min
with longer column , column back pressure increases
Thankyou ma'am, for HPLC method development explain in easy way 😊
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Can you explain what is column back pressure
Column back pressure is back pressure due to Column. As mobile phase passes through Column containing stationary phase of fine particle (3 micron or 5 micron), the pressure is generated.
Very nice mam
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Very good
Thanks
Super explanation
Thank you
Good
Good video for team thank you
Great lecture.keep it up
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