The acid fast stain

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ความคิดเห็น • 7

  • @ahmedsumaiyah9324
    @ahmedsumaiyah9324 7 ปีที่แล้ว +1

    Really helpful for my practical.thanks!

  • @HashemSmashem
    @HashemSmashem 6 ปีที่แล้ว +2

    Nice demonstration. Shouldn't you be wearing gloves? And wouldn't the heat from the steam denature the bacteria?

    • @timothypaustian3852
      @timothypaustian3852 6 ปีที่แล้ว +1

      Thanks for the comment. Nope, no gloves. During this procedure, a scientist can wear gloves if you are concerned about getting the stain on your hands, but I would rather they were careful enough that this wasn't an issue. Gloves are wasteful and make you sloppy. There are times when it's a good idea to wear gloves, such as when you are working with pathogens or if you are using toxic chemicals.

    • @HashemSmashem
      @HashemSmashem 6 ปีที่แล้ว

      @@timothypaustian3852 Thanks for the reply! That makes sense. Great humor in the video haha.

    • @BigWalkinTallV
      @BigWalkinTallV 5 ปีที่แล้ว +1

      @@timothypaustian3852 Which acid/alcohol solution is used for this video? I'm confused at your stance on gloves, the typical 20% H2SO4 solution is - according to the SDS - able to "cause severe burns to skin and eye damage". Why would you choose to avoid wearing standard PPE for this? Maybe you're using... something else that isn't corrosive to skin? I've also never seen any pair of properly fitted latex gloves cause any noticeable loss of dexterity, hence their use in surgery and pretty much any legitimate lab environment. Latex gloves actually increase grip while holding wet glass. Not trying to troll you, I'm genuinely confused at why gloves are not being used while handling acid.

    • @paustian831
      @paustian831  4 ปีที่แล้ว

      @@BigWalkinTallV First, they are unnecessary. If you are careful, the solution doesn't get on your hands. If you are wearing gloves, you don't have to worry about it, and if you do get something on your hand, it is less likely that you will notice. Then when you touch something else you spread it on that surface, etc. If it does get on your hand without gloves you will notice and wash it off immediately. Second, it's wasteful, especially in a teaching laboratory. Instead, we provide forceps or clothespins that students can use to hold the slide at that time. You will notice in the video the experimenter does not get any stain on their fingers during the procedure.