Bro....app bhout acha ho....baki sabhi channels pa bo din ki sirf ik video upload krta ha....app din ki....2,3 video upload kr deta ho....broo...app sch ma bhout pyara...aur kind heart ho....sch ma....❤😅app ka liya jitna bola utna kam ha.....❤❤
AIM : To perform gram staining of given sample. MATERIAL REQUIRED : Glass slides, bunsan burner , cotton , sample , microscope. REAGENTS REQUIRED: Crystal violet dye, iodine, alcohol (95% ethyl alcohol), saffranin dye PRINCIPLE: Gram staining is most widely staining technique used in m/o examination. It was discovered by Danish scientist and physician Hans Christain Joachin Gram in 1884. This technique differentiates bacteria in 2 groups i.e. Gram positive and Gram negative bacteria. The procedure is based on the ability of m/o to retain colour of the stain during Gram reaction. Gram negative bacteria are decolourised by alcohol losing the colour of primary stain , purple. Gram positive bacteria are not decolourised by alcohol and will remain as purple. After decolourisation stop , a counter stain is used to impart pink colour to the gram negative m/o. Gram positive bacteria have a thick mesh like cell wall which is made up of peptidoglycan (50-90%) of cell wall, which stain purple. Gram negative bacteria have a thinner layer of peptidoglycan (10% of cell wall) and lose the crystal violet iodine complex during decolourisation with alcohol rinse but retain the counter stain safarin thus appearing reddish or purple. STAIN REACTION : 1. Application of crystal violet to heat fixed smear : CV dissociates in aqueous solution into CV+ and Cl- ions. These two penetrate the cell wall and cell membrane of both gram positive and gram negative . CV+ interact with negative component of bacterial cell and stain it purple. 2. Addition of gram iodine : Iodine acts as a mordant and a trapping agent. A mordant is a substance that increase the affinity of cell wall for a stain by binding to primary stain , thus forming a insoluble complex that get trapped in cell. During the reaction CV-I compex is formed and all the cells turn purple. 3. Decolourization with ethyl alcohol : Alcohol dissolve the lipid outer membrane of gram negative bacteria, thus leaving the petidoglycan layer exposed and increase the porosity of cell wall. The CV-I complex is then washed away from the peptidoglycan layer leaving gram negative bacteria colourless.In gram positive bacteria , alcohol has dehydrating effect on cell wall causing cell wall to shrink , then CV-I complex get tightly bound into multi layered leaving the cell with purple colour. 4. Counter stain with safranin dye : The decolourised gram negative cell can be visible with a suitable counter stain which is usually positively charged safarnin, which stained it pin
Episode 17 👇
th-cam.com/video/PCi-CPxvjjg/w-d-xo.html
Episode 18 👇
th-cam.com/video/GGIEG9nOX5c/w-d-xo.html
Drama name?
Please be my family
@@Jmtdyx❤ please be my family ❤
Your voice is very good
Nice explanation 💕💕
Bro your voice too good 😊😊😊😊
Video kya mujha toh aapki voice bhi bohot pasand hai sachin ma bro❤️
Thanks for this episode 🤗🤗🤗🤗🤗
Babys is to cute😊
14:30 nice story 🥰😘🥰😍🥰😘🥰😍🥰😘🥰😍🥰😘🥰😍🥰😘🥰😍🥰😘🥰😍🥰😘🥰😍🥰😘🥰😍🥰😘🥰😍🥰😘🥰😍🥰😘🥰😍🥰😘🥰😍🥰😘🥰😍🥰😘🥰😍🥰😘🥰😍🥰😘🥰😍🥰😘🥰😍🥰😘🥰😘🥰😘
Your voice is so nice ❤
We love you voice and explanation very much love for Jharkhand💕💕💕💕
I love from Mumbai
So nice drama bhai and next part 17 explain jaldi se 💜💞💜💞💜💞💜💞💜💞💜
So lovely drama 💗💘
Thanks for this part next episode please
Nice daram 💞💞🧡🧡💚💚🧡💚💚🧡💚💚🧡💚💚🧡💚🧡 sooooooo beautiful drama 💖💙💖💙💖💙💖💙💖💙💖💙💖💙💖💙💖💙💖💙💖💙
Thanks for this episode ☺️ I'm wetting for next episode
U r awesome 👍
Ap bht acha explain krty ho ❤
Please bro next part❤❤❤😊😊
Next ❤part 17,❤😊 18 ❤ please ❤🙏🏻
Bro....app bhout acha ho....baki sabhi channels pa bo din ki sirf ik video upload krta ha....app din ki....2,3 video upload kr deta ho....broo...app sch ma bhout pyara...aur kind heart ho....sch ma....❤😅app ka liya jitna bola utna kam ha.....❤❤
The way when he said: Kya sach Mai .
I was like "what"
First commenter🤭
Bro your voice. So good
Love bro
Bro your voice. So good .is not correct Bro your voice is so good
Please be my family
So nice drama ❤️❤️❤️😍
❤❤❤
6:50 😊
Thank you thank you so much 🇮🇳🇮🇳
Thank you for this episode waiting for next episode💜
So cute❤
Thank you and next part jaldi hi upload Karo plz jaldi 💜💞💜💞💜💞💞
Nice 😍
Thank you 👍
Next part please🙏🙏🙏
Bhaiya King the land plzz upload ki jiye nh
Nice
Nice drama ❤❤❤❤
Yese ladke milte kha hai Bhai mujhe bi chahiye dedo mujhe bi please 😢😢
Bro we are awaiting for next part please upload it quickly... Please 🥺
Yarr yah bohot long story he ese dakhte dakhte nid ah jata h .... Ese tw movie bolna sahiye😂😂😂
Please part 19😮
Please video upload at 9:00pm
Fast 😊
When will you upload part 17??
Hi bro
Nice ❤❤
Fan from pakistan 😊
🎉
Vaia apnar expretion shunte amar onek valo lage
💜💜
❤❤❤❤😊😊😊😊😊😊😊😊😊😊😊😊 nice movie
Nice video and stro
❤❤
hindi episode plz 😢
Naxt pat
So handsome boy 😅😊
❤❤❤❤❤🎉
❤️🧡💛💚💙💜❤️🧡💛💚💙💜
17 episode please
Bhaiya ya Drama full video do na
I love you too
Plz next episod de dije
Bro 3 part
Drama nema: pleas be my fimily
😊😊
PSJ bhai part 17 kab aayega 😢
Don't worry 9 PM Tak aa jayega
@@psjexplainshindi thankyou jaldi video upload krne ke liye ☺️☺️
Drama name please
Please be my family
Please be my family ❤
Which app you use for editing plz reply ??
Youcut
@@psjexplainshindi ise puri long video edit hojati h 1-2 ghnte ki video plz rply ?
Next part
Next pat
What is the name of the drama
Next part?
👍👏
❤
Next part kab ayega
5th june
@@psjexplainshindi acha ap bht acha explain karty ho maza ata he sun ny mn apki fan hun from pakistan
🤣🤣🤣👍👍
ngl but his the Chinese version of yeonjun 🤭🤍
Drama name 📛
Please Be My Family
Series ka kya naam ha kon mujha batao
Ya series Hindi ma ha ya nahi ha
Please Be My Family aur ye Hindi dubbed me available nhi hai
Bhai iss tarah ki gaming app ka promotions naa kare kyonki isse kewal paisa barbaad hai
Okay I'll Keep it in mind
❤❤gg
❤😢😮😊
Judul dramax
Lusi and qisi
Ye drama konse app per dhek skte hai hindi dubbed me
Yeh toh dekchuki ho
Don't worry 9 PM Tak video aa jayegi Psj Bhai ne Kha
Drama name: please be my family
______💖💖💖💖💖💖______
🤔🤔🤔
DRAMA name ??
AIM : To perform gram staining of given sample.
MATERIAL REQUIRED : Glass slides, bunsan burner , cotton , sample , microscope.
REAGENTS REQUIRED: Crystal violet dye, iodine, alcohol (95% ethyl alcohol), saffranin
dye
PRINCIPLE: Gram staining is most widely staining technique used in m/o examination. It
was discovered by Danish scientist and physician Hans Christain Joachin Gram in 1884. This
technique differentiates bacteria in 2 groups i.e. Gram positive and Gram negative bacteria.
The procedure is based on the ability of m/o to retain colour of the stain during Gram
reaction. Gram negative bacteria are decolourised by alcohol losing the colour of primary
stain , purple. Gram positive bacteria are not decolourised by alcohol and will remain as
purple. After decolourisation stop , a counter stain is used to impart pink colour to the gram
negative m/o.
Gram positive bacteria have a thick mesh like cell wall which is made up of peptidoglycan
(50-90%) of cell wall, which stain purple. Gram negative bacteria have a thinner layer of
peptidoglycan (10% of cell wall) and lose the crystal violet iodine complex during
decolourisation with alcohol rinse but retain the counter stain safarin thus appearing reddish
or purple.
STAIN REACTION :
1. Application of crystal violet to heat fixed smear :
CV dissociates in aqueous solution into CV+ and Cl-
ions. These two penetrate the cell
wall and cell membrane of both gram positive and gram negative . CV+ interact with
negative component of bacterial cell and stain it purple.
2. Addition of gram iodine :
Iodine acts as a mordant and a trapping agent. A mordant is a substance that increase
the affinity of cell wall for a stain by binding to primary stain , thus forming a
insoluble complex that get trapped in cell. During the reaction CV-I compex is formed
and all the cells turn purple.
3. Decolourization with ethyl alcohol :
Alcohol dissolve the lipid outer membrane of gram negative bacteria, thus leaving the
petidoglycan layer exposed and increase the porosity of cell wall. The CV-I complex
is then washed away from the peptidoglycan layer leaving gram negative bacteria
colourless.In gram positive bacteria , alcohol has dehydrating effect on cell wall
causing cell wall to shrink , then CV-I complex get tightly bound into multi layered
leaving the cell with purple colour.
4. Counter stain with safranin dye :
The decolourised gram negative cell can be visible with a suitable counter stain which
is usually positively charged safarnin, which stained it pin
😂😂😂 jb TH-cam b use krn h pr kl exam b ho
😂
baeed
Is drama ka naam kya hai
Please be my family
Thanks for uploading waiting for next episode ❤❤❤❤
Next part please
Next episodes
Drama name please
Please be my family
❤❤❤
Whats drama name ?
Please Be My Family
❤