Primer Designing using Primer3web tool

[@mohammadtorikh]

alhamdulillah, thank you sir

[@PakistanScienceTV]

Thanks

[@khalidabdullah2561]

Thank you for your explanation😃

[@abdullahsaif2980]

Assalam o Alaikum from the first can you explain plz? How to get the sequence?

[@PakistanScienceTV]

please follow these links
th-cam.com/video/q5lvuymtnP8/w-d-xo.html&ab_channel=MuzammilAhmadKhan
th-cam.com/video/ntEYVR1OoMQ/w-d-xo.html&ab_channel=MuzammilAhmadKhan
th-cam.com/video/LySmO7zKLUA/w-d-xo.html&ab_channel=MuzammilAhmadKhan

[@monkaydgoofy2059]

if there isn't enough introns can we take few bases from the next exon

[@PakistanScienceTV]

Yes you can sequence from upstream and downstream region

[@monkaydgoofy2059]

@@PakistanScienceTV ok thanks. btw nice video. subbed

[@unemediterraneenne1026]

Thank you so much!

[@snehalkapse1843]

DID YOU USE FASTA FILE TO COPY THE SEQUENCE

[@PakistanScienceTV]

No, we used plain sequence. I hope, I answered your question? if not then elaborate your question I will explain it further.

[@rensfrank3905]

Thank you!

[@sahar9314]

Thank you but I wish you cleaned your throte prier the video capture