Cuál es tu recomendación para el traspaso de cultivo a otros medios, si tiene presencia de hongos recomiendas desinfectarlo con antifúngico y luego al otro medio? O no usarlo?
Yes, prefer to remove all the damaged and infected parts, surface sterilize them again, and then transfer them to the fresh media. You can add PPM to prevent contamination. However, choosing a healthy mother plant is always recommended for a higher success rate.
We have; check out our channel. In addition, you can also check out our blogs, having loads of info on the subject: plantcelltechnology.com/blogs/blog/tagged/for-beginners
Hi, where are you located? Though antibiotics are used as an alternative, generally, microbes get resistant to them over time. So, the other thing you can do is keep a strict aseptic environment while performing the tissue culture experiments.
@@PlantCellTechnology I’m from Ukraine , but now I am in Russia. Can I buy it ppm from you? Can you send me ppm in here? How can I contact you? Maybe email, telegram? Thank you .
@@Bambu_Gigante_Mijas No shoot regeneration comes next. The initiation stage is when tissues are transferred to a lab condition in a media and allowed to grow without any hormones. You can learn about all the stages of tissue culture here: plantcelltechnology.com/blogs/blog/blog-four-stages-of-micropropagation?srsltid=AfmBOoodv-Rxzfl2vkd2CiwUQHfQYw1nrNMeZ8XSOVVaXoH9mNVPed1k
Prezado, o seu vídeo é fantástico, extraordinário. Um dos melhores do youtube. Obrigado!
Thank you so much for your love and support!
Cuál es tu recomendación para el traspaso de cultivo a otros medios, si tiene presencia de hongos recomiendas desinfectarlo con antifúngico y luego al otro medio? O no usarlo?
Yes, prefer to remove all the damaged and infected parts, surface sterilize them again, and then transfer them to the fresh media. You can add PPM to prevent contamination. However, choosing a healthy mother plant is always recommended for a higher success rate.
Amigo..muchas gracias..como se llama lo que va después de el azúcar?se escucha entrecortado
La media MS
Qué es la ms
@@marcelacastroduran5544 Murashiege and Skoog Media.
Muy buen video.
¿Hay alguno que muestre el equipo necesario para hacer cultivo en casa?
We have; check out our channel. In addition, you can also check out our blogs, having loads of info on the subject: plantcelltechnology.com/blogs/blog/tagged/for-beginners
@@PlantCellTechnology Thanks
Hi! I can not buy ppm in my county , how I can replace ppm?
Hi, where are you located? Though antibiotics are used as an alternative, generally, microbes get resistant to them over time. So, the other thing you can do is keep a strict aseptic environment while performing the tissue culture experiments.
@@PlantCellTechnology I’m from Ukraine , but now I am in Russia. Can I buy it ppm from you? Can you send me ppm in here? How can I contact you? Maybe email, telegram? Thank you .
@@PlantCellTechnologyHow can I contact to you? Maybe e-mail, or telegram?
Quisiera saber cómo los esterilizas en la olla y en el microondas
Sterilize the media? We have many videos on our channel about it. Please check them out.
Ya y despues que sigue..se riegan plantas con tal solución ó mezcla...???
¿Estás preguntando sobre el proceso? ¿Qué sigue después de cultivar plantas de cultivo de tejidos?
Gracias por hacer el video en español también ;D un saludo
¡Por supuesto! ¡Espero que te haya gustado!
He visto que no has añadido ningún regulador de crecimiento, cierto? Cual es el motivo?
We don't require growth regulators for the initiation stage. However, we need them for root and shoot regeneration.
@@PlantCellTechnology do you mean "initiation stage" for example, shoot induction?
@@Bambu_Gigante_Mijas No shoot regeneration comes next. The initiation stage is when tissues are transferred to a lab condition in a media and allowed to grow without any hormones. You can learn about all the stages of tissue culture here: plantcelltechnology.com/blogs/blog/blog-four-stages-of-micropropagation?srsltid=AfmBOoodv-Rxzfl2vkd2CiwUQHfQYw1nrNMeZ8XSOVVaXoH9mNVPed1k
en tu experiencia cual debió usar? soy muy nuevo en esto, saludos.
@@albatrossestramonium4326 Bye "which one", do you mean plant hormones?
Al dar tercer ingrediente el audio se distorsonió x lo que ignoramos su nombre...el agar donde lo compro...
Can you tell the time point?
No dice nada de como se usa la solución...
No sé le entiende nada ,solo puede repetir sería bueno