I think a valuable addition in terms of the diagnostics on this subject is that there are well-established PCR techniques for more rapid detection and very precise species determination in cases where 'ample' sample materials can be taken, compared to the classical growth/microscopy methods, even when they also involve MALDI-TOF identification on early growths. I work myself as a biomedical laboratory scientist at a department of clinical microbiology where one of my areas of expertise and specialization is to perform such tests and train personnel in the sample handling and result evaluation, as well as managing the maintenance of machinery and probing the soundness of our testing. We generally use a two-step PCR involving a screening stage for the most common singular dermatophyte along with a pan-dermatophytic indicator detection, and a speciation stage for the less-typical species seen in our country, following a sample lysis and extraction, of course. Firstly, we lyse the sample using a buffered solution of proteinase K to soften keratinous tissues in order to liberate into the solution as many buds and hyphae as is reasonably possible when we then vortex the sample after a 2-4 hour 55 degree heating. Then the DNA from the lysate is extracted using an automated sample processing system, and the resulting elution is first run through the screening PCR containing an in-house primer set targetting the trichophytom rubrum species along with an inhibition control, and a generic pan-dermatophytic target along with the same inhibition control in parallel. Many samples are then found to be positive for the trichophyton rubrum species to a satisfying degree immediately in the screening, and some are found to be neither positive for trichophyton rubrum nor the pan-dermatophytic target and are concluded as negative samples. Then any inhibited samples indicated by the control addition and samples indicative of either a) only the pan-dermatophytic target, or b) both the trichophyton rubrum as well as the pan-dermatophytic target with
Thanks Sir. Very precise and well put, can be used as a supplement for my readings on Jawetz Medical Microbiology on mycology - superficial (tinea versicolor and nigra) and cutaneous (dermatophytes) mycoses.
Im from iran My daughter, her scalp is infected with an infected cat, but it does not get better. I use Binafine tablets and antifungal shampoo daily😥.
I think a valuable addition in terms of the diagnostics on this subject is that there are well-established PCR techniques for more rapid detection and very precise species determination in cases where 'ample' sample materials can be taken, compared to the classical growth/microscopy methods, even when they also involve MALDI-TOF identification on early growths.
I work myself as a biomedical laboratory scientist at a department of clinical microbiology where one of my areas of expertise and specialization is to perform such tests and train personnel in the sample handling and result evaluation, as well as managing the maintenance of machinery and probing the soundness of our testing.
We generally use a two-step PCR involving a screening stage for the most common singular dermatophyte along with a pan-dermatophytic indicator detection, and a speciation stage for the less-typical species seen in our country, following a sample lysis and extraction, of course.
Firstly, we lyse the sample using a buffered solution of proteinase K to soften keratinous tissues in order to liberate into the solution as many buds and hyphae as is reasonably possible when we then vortex the sample after a 2-4 hour 55 degree heating.
Then the DNA from the lysate is extracted using an automated sample processing system, and the resulting elution is first run through the screening PCR containing an in-house primer set targetting the trichophytom rubrum species along with an inhibition control, and a generic pan-dermatophytic target along with the same inhibition control in parallel.
Many samples are then found to be positive for the trichophyton rubrum species to a satisfying degree immediately in the screening, and some are found to be neither positive for trichophyton rubrum nor the pan-dermatophytic target and are concluded as negative samples.
Then any inhibited samples indicated by the control addition and samples indicative of either a) only the pan-dermatophytic target, or b) both the trichophyton rubrum as well as the pan-dermatophytic target with
Thank you for these videos. They are great!
excelent video and clear explanation, thank you
Very simplified presentation. Thanks
Excellent presentation and explanation. Just discorvered your channel. Thank you.
Thanks Sir. Very precise and well put, can be used as a supplement for my readings on Jawetz Medical Microbiology on mycology - superficial (tinea versicolor and nigra) and cutaneous (dermatophytes) mycoses.
How does anyone find doctors to help us get over this?
Thanks.. easy but scientific✋🏼
For treatment you didn't include Tinea Manuum? Or am I missing it somewhere
Thank you☺️
Nice.. thanks
Why did you stop making vids? These are so damn good
Im from iran My daughter, her scalp is infected with an infected cat, but it does not get better. I use Binafine tablets and antifungal shampoo daily😥.
try carnivore diet. may take months before improvement
Thank you for these videos. They are great!