AGROBACTERIUM MEDIATED GENE TRANSFER | CSIR NET | GATE | M.Sc. | B.Sc.|
ฝัง
- เผยแพร่เมื่อ 11 ส.ค. 2018
- TOPIC : AGROBACTERIUM MEDIATED GENE TRANSFER For CSIR NET/GATE/M.Sc./B.Sc.
A. tumefaciens is a rod-shaped, Gram-negative soil bacterium which infect dicot plants.
Some common species of Agrobacterium are- A. tumefaciens and A. rhizogenes.It is consider as natural genetic engineer because they transform the plant.
To be virulent, the bacterium must contain a tumour-inducing plasmid (Ti plasmid or pTi), which contains the T-DNA and all the genes necessary to transfer it to the plant cell.
The mechanism this bacterium uses to parasitize plant tissue involves the integration of some of its own DNA into the host genome resulting in tumors and changes in plant metabolism.
#AGROBACTERIUMMEDIATEDGENETRANSFER #Agrobacteriumtumefaciens #CSIRNET
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Really superb and in detail explanation......its a crystal clear explanation..no need to refer any text book...
No need to read so many sentences in book. You taught it all in few minutes. Thank you ma'am. 😊 keep growing.
Thank you ma'am for making all the topics so easy, if I clear csir net, I hope I become a lecturer like you! You are an inspiration!
Awesome explanation...nice explanation...the way of your teaching is suitable for me... really helpful video...thank you mam
Simple but very informative lecture in a short time👍
Thnk u so much mam.👏👏👏👏..i was reading this topic from 1 hour (from pdf) and this 18 minutes video cleared my whole concept.......the best part of ur teaching is d application of cncpt in qstions dat u discss in d last of evry cncpt (on u tube nd unaccdmy as well) ...nd dat is vry hlpful mam....💖💖💖💖
This work is praisable beautifull explanation
Mam ur video was awesome..nd it is vry hlpful..i am preparing 4 BT gate exam. My concept is absolutly clear after watching ur video..thanku mam
Very easy to understand Madam. Thank you.
Another outstanding video....
Thank u very much ma'am
Mam your videos are really supportive for me to prepare for csir net...Plz upload some more videos on various techniques in last unit. I really find your classes so helpful and im understanting every points in your lecture
i will try my best to help u all.
keep supporting.
Mam ur videos are really helpful to me for csirnet tanq alot mam
Small request mam upload few more techniques from last unit mam plzzz
Excellent explanation all doubts get cleared thank you mam👍🙏
In Both Teaching patshala & unacademy ur concept explanation is good...thanks
Nice explanation. Thank u so much.
Awesome. Thanks a lot
Nice explanation in short period of time ..
ma'am please upload some videos relatable to Fundamental Process. Because there is lots of thing in it to recognize but whats really important from exam point of view hard to find out
Yesterday, Just before my GATE exam I learned this from you mam. And it came. And I remembered because you are so good of a teacher. Thanks for always.....always helping me out with many topics🤩🤩🤩🤩🤩🤩🤩
Great👍
This video helps me a lot....👍👍👍
your way of teaching is very nice mam......
m a subscriber of ur channel from few days before nf m getting a big fan of urs. d way u explain every topics are excellent.love it.
Thank you!
Thank you so much. It really helped a lot.
bravo! keep it up mam!
Thanku soo much mam😊,, really very helpful 😊
Thnkxxx a lot mam have done wel ....👍
Thank you so much for your perfect video 😘😘😘😘😘
such a wonderful efficient lecture mam..lots of thanks from tamilnadu ..mam plz make videos on gene regulation in prokaryote and eukaryote
Wow
Your video too good mam can you please make a video of R.D.T and immunology
Thanks mam I just saw the video and able to write whole answer
in exam without reading once....😄
Thnq so much ma'am....pllz ma'am make a vedio on nitrogen fixation and it's cycle....it's important for CSIR NET JRF...... ma'am plant physiology meko kabhi achhi ni lgti...but after watching ur vedio....my oll concepts is clear....thnkx for helping me n every students..lots of thanks..
welcome!!
Thanku mam 😊👍🌹🌹
Thank ou. My question is does rRight and left border comes under TDNA or they are different?
Thanks ma'am 😊👍
Thank u miss 😊
Thankuuuuuuuu❤️
Tysm
Thank you madam.. Pls make video on secondary metabolites which will be highly useful...
sure!!
@@TEACHINGPATHSHALA Thank you madam..
Mam, nos gene codes for nopaline synthase..then its function is nopaline biosynthesis not octapine biosynthesis..
Please correct me if I'm wrong!!!
Thanx
Thank u so much maam
Ma'am I have a doubt about the slide showing examples of T-DNA genes in Ti plasmid, It is written that function of nos gene product Nopaline synthase is Octopine synthesis.. Is it actually synthesis of Octopine or Nopaline?
It will be opine. I have taken the help of Pathfinder book, in that it has been written opine .Nopaline is a chemical compound derived from the amino acids glutamic acid and arginine and is classified as an opine.
@@TEACHINGPATHSHALA Thank you😊
Ma'am still you are not answering to the question ! You have mentioned nos produce NOPALINE SYNTHASE and its function as OCTAPINE SYNTHESIS. Is this correct?
Nos gene product is nopaline synthase and function is nopaline synthesis
💝💝💝💝you are always 💝
Mam from where to study applied biology module ...
😍😍👍
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All cleared
Ma'am .....what is the role of the phosphorylated Vir G dimer in this transformation
Thank you so much 🥰 mam
Hi... Its very informative and easy to understand. Just want to know to which unit of csir syllabus this topic belongs? Please let me know
u may get this topic in plant biology unit as well as in techniques unit.every year atleast there is a minimum 1 question in csir net from this topic.
@@TEACHINGPATHSHALA Thanks for the quick response. Your videos are a very good support for me to prepare.. For csir net. Thanks once again
u r most welcome!!
keep supporting..
how can we use Ti and Ri plasmid as a vector , please make video on this...
nopaline synthase forms Nopaline, you mentioned wrong in your slide. In next video of csir questions also mentioned this.
@10:00 nos doesn't sunthesize octapine, it's nopaline
I have a qu. Mam..
Nopaline synthase enz. produces Nopaline or Octapine ?
Bcoz u mention in slide that Nopaline synthase produces Octapine...
Plz clear .
what every mentioned in slide, it is correct
Mam I got only 30 marks... In June 2019 ,but I have studied thoroughly.... Now even I dare not to face csir net . What should I do mam? Getting frustrated please help me
Ma'am u left 2 proteins chvA and chv B protein
Describe the Agrobacterium mediated gene transfer mechanism in plants mutation and how it distinguished by other types of mutation? Plz explain this ques what I hv to write in that
Mam is this lecture is also available in hindi
Mam Hindi me bhi padaiye please
Mam i have enrolled your course on unacademy. But didn't find this topic.my exam is there. Please help.
mam you explain very nice but how I will make the notes thats the problem can you please help me for the notes on the steps in transformation of plant cell
Please mam
Ma'am plz help me in unit 1... this is really difficult portion for me. Wht r d imp topics plz make a vdo on tht
I have already uploaded a video on this topic. do watch that.
There is a question asked from this topic ..can u solve it...ques no 137 from December 2015 NET
The showing of topic really good....but it is too fast to follow....🙂🙂
u can slow down the speed by youtube setting
Mam agrobacterium mediated gene transfer ka part-2 video nhi mil pa raha h kya aap link bhej sakte h
till not it is not available
Very informative but I would be better if you could explain a little bit slower.
Okay
Usually ur videos were tooo good but this time something happens u r just reading and going
U NEVER REPLY ..PL RPLY U MENTIONED many techniques but explain sirf agrobacterium kia to baki tech imp ni h kya plz btaye
All techniques are important. Due to less availability of time mai baki techniques pe lecture nahi bana paa rahi.
VirD1 acts as topoisomerase...also.
In December 2017, 2 questions were asked in C section for 8 marks on this topic only ...
There is a one more video on this topic, in that I have solved many previous year CSIR NET questions related to this topic.
th-cam.com/video/Zhnpao--wvs/w-d-xo.html
Amphitrichous is wrong, or said it is not properly right here, becoz when there bunch of flagella on both the ends i.e said to be amphitrichous Flagellar bacteria.
It may be bunch of flagella or single flagellum. both condition are applicable.
@@TEACHINGPATHSHALA thanku ji
Ma'am aap phle ni aa skte the 😫
Ab aa gai hu, :-) and I am trying my best.
@@TEACHINGPATHSHALA 💓💓💓
Slower the speed pls
Sure!!
I can't understand with that much of speed... Make a slow motion video.. Then people are understand..
Sorry for inconvenience caused.
Current videos are with correction in speed.
Kindly slow down the video speed from TH-cam seetings, and then watch.
Mam please avoid saying "of a" in every video even it is in plural form.
Mam hindi mi jada samaj mi aata
Plz talk slowly mem
Etna fast reading krti hain .
There was a problem in my recording app that time, so it lead to the increase in my speech.. now I used to make videos in normal speed. do adjust the speed with youtube setting for the video, in which you are facing the same problem.