The important part of the Ames test is mixing the test compound with rat liver extracts which contains mixed function oxidase that converts promutagens into mutagens.
@3:14 sorry sir.. but to overcome this disadvantage.. we should place a liver homogenate of rat into culture medium.. cuz that way even if promutagen is to be tested then enzyme cytochrome p450 released from liver extract will metabolically activate the promutagen to mutagen.. and then the mutagen can be detected...
Be aware of spontaneous revertans. There will always be some spontaneous revertants, even if the chemical substance is not mutagenic. This can be a way of controlling yourself. If this is not the case, then you made a fault in your experiment.
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The important part of the Ames test is mixing the test compound with rat liver extracts which contains mixed function oxidase that converts promutagens into mutagens.
@3:14 sorry sir.. but to overcome this disadvantage.. we should place a liver homogenate of rat into culture medium.. cuz that way even if promutagen is to be tested then enzyme cytochrome p450 released from liver extract will metabolically activate the promutagen to mutagen.. and then the mutagen can be detected...
In the original paper they used human liver homogenate I think
Be aware of spontaneous revertans. There will always be some spontaneous revertants, even if the chemical substance is not mutagenic. This can be a way of controlling yourself. If this is not the case, then you made a fault in your experiment.
Thanks For the detailed feedback
It was good lecture 👍got idea of ames test
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Thank you very much Mr shomu . I appreciate you
But I am not Shomu not cumar
Really? Was it so easy? Thank u verry much.... for this video. Keep it up..... and ur voice 👌👌👌
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Really good to know that you found it useful
Thank you very much Mr Cumar . I appreciate you.
yes we can detect promutants, by using a liver extract
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Great . Thankyou so much . Very informative
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Thank u sir. Nice vedio
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thanks good explanation
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Thank you sir...please upload another test like,,,1)Fluctuation test 2)commet assay..etc
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@@animatedbiologywitharpan thank you sir
Thank you a lot.
ترجمة باللغة العربية بليز 😫
Thank You!!!!
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Thank u sir
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Thnkyouúi
Mmm .😊
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@@animatedbiologywitharpan worry about 🙏🙏