On 'Individual 1' wouldn't fragment lengths 1 and 3 still be attached to the DNA at that specific loci given the restriction enzyme hasn't cleaved the DNA strand before fragment 1 or after fragment 3? Same goes for the second individual? I thought that VNTRs were cleaved at the flanking region sequence sites before and after the tandom repeating DNA sequences?
sir firstly thank you for the wonderful explanation. I want to ask how I understand what is mean by heterozygous, mutant, and wild type result written in research papers after doing RFLP on PCR basis? please explain
Maryam, Restriction enzymes are very specific and cleave a particular sequence only which are called as Restriction site. Each individual has different number of occurrences of these Restriction sites therefore different lengths of DNA are obtained. Hope this helps.
Sir you really make us understand please make some more video about it really appreciated for your effort to make us unsderstand
On 'Individual 1' wouldn't fragment lengths 1 and 3 still be attached to the DNA at that specific loci given the restriction enzyme hasn't cleaved the DNA strand before fragment 1 or after fragment 3? Same goes for the second individual? I thought that VNTRs were cleaved at the flanking region sequence sites before and after the tandom repeating DNA sequences?
thanks a lot i clear my confussion
Thank you sir
Thank you for the presentation,it has really been helpful
VERY Nicely explained
please exlpain DNA foot printing
Thankyou sir!!
sir firstly thank you for the wonderful explanation. I want to ask how I understand what is mean by heterozygous, mutant, and wild type result written in research papers after doing RFLP on PCR basis? please explain
Very nice explanation
Understandable explanation
Tysm guru ji....
Yes sir ur class is useful tq sir
Thank you for the appreciation.
Sir why restriction enzymes gives different lengths of DNA ?
Maryam, Restriction enzymes are very specific and cleave a particular sequence only which are called as Restriction site. Each individual has different number of occurrences of these Restriction sites therefore different lengths of DNA are obtained. Hope this helps.
Very useful to me.
Thanks a lot Sir. I request you to kindly explain role of RFLP in disease detection
Thanks you sir
Thank You for the appreciation
How do u decide the sequence of the probe?
Thank you so much sir
Thank you sir
Thank u sir